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[188199_s_at]

Description C15F1.3B /REP_DB=WormBase Gene ID /WP=CE23547 /GEN=tra-2 /TR=Q9N5Y5 /GB=AAF39756.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 46      4grp_total > 11      6grp_cgc > 35      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 43.6
(-0)
M 		49.8
(0.4)
A 		3.4
(-3)
A 		84.3
(3)
A 		81
B 37.2
(-3)
A 		59.3
(1.5)
A 		66.6
(3)
P 		50.1
(-0.4)
A 		29
C 47.1
(-0.9)
A 		26.9
(-3)
A 		41.9
(-1.4)
A 		83.9
(3)
A 		57
avr 42.6
(-2.1) 		45.3
(-1.6) 		37.3
(-3) 		72.8
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 76.6
(3)
A 		37.2
(-1.8)
A 		33
(-2.3)
A 		39.4
(-1.5)
A 		27.3
(-3)
A 		63.5
(1.4)
A 		49
B 67
(3)
A 		42.3
(0.6)
A 		60.8
(2.4)
A 		54.8
(1.8)
A 		5.2
(-3)
A 		24.5
(-1.1)
A 		62
C 51.8
(2.3)
A 		40.3
(0.9)
A 		48.1
(1.9)
A 		13.9
(-2.3)
A 		8.6
(-3)
A 		57.3
(3)
P 		49
avr 65.1
(3) 		39.9
(0.1) 		47.3
(0.9) 		36
(-0.4) 		13.7
(-3) 		48.4
(1.1) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 15.9
(3)
M 		10.8
(1)
A 		0.9
(-3)
A 		12.3
(1.6)
A 		15
B 11.9
(-1.5)
A 		14.7
(2.1)
A 		15.4
(3)
P 		10.7
(-3)
A 		5
C 15.6
(3)
A 		7.7
(-3)
A 		14.6
(2.2)
A 		14.1
(1.9)
A 		8
avr 14.5
(3) 		11.1
(-1.9) 		10.3
(-3) 		12.4
(0) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 14.1
(2)
A 		13.2
(1.3)
A 		7.2
(-3)
A 		10
(-1)
A 		8.2
(-2.3)
A 		15.5
(3)
A 		8
B 23.3
(3)
A 		14.1
(0.4)
A 		14.8
(0.6)
A 		14.5
(0.5)
A 		2.1
(-3)
A 		9.1
(-1)
A 		21
C 19.2
(3)
A 		12.3
(0.5)
A 		12.2
(0.4)
A 		2.8
(-3)
A 		2.8
(-3)
A 		19.2
(3)
P 		16
avr 18.9
(3) 		13.2
(0.6) 		11.4
(-0.1) 		9.1
(-1.1) 		4.4
(-3) 		14.6
(1.2) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188199_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5861
Sequence Type Exemplar sequence
Representative Public ID C15F1.3B NCBI
Target Description C15F1.3B /REP_DB=WormBase Gene ID /WP=CE23547 /GEN=tra-2 /TR=Q9N5Y5 /GB=AAF39756.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026783(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026783 NCBI Caenorhabditis elegans TRAnsformer : XX animals transformed into males family member (tra-2) (tra-2) mRNA, complete cds. 11/11 None
C15F1.3b ENSEMBL cdna:known chromosome:CEL140:II:6957981:6965618:-1 gene:C15F1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174137 2 1 NM_001026783 188199_s_at (11)
NM_063025 173449_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6958061-6965600(-) 100.0 100.0
Public Domain and Genome References
Ensembl C15F1.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39756 Transformer : xx animals transformed into males protein 2, isoform b [Caenorhabditis elegans] ref|NP_001021954.1| TRAnsformer : XX animals transformed into males family member (tra-2) [Caenorhabditis elegans] 0.0
blast AAF39755 Transformer : xx animals transformed into males protein 2, isoform a [Caenorhabditis elegans] ref|NP_495426.1| TRAnsformer : XX animals transformed into males family member (tra-2) [Caenorhabditis elegans] gb|AAB22845.1| pTra2A [Caenorhabditis elegans] sp|P34709|TRA2_CAEEL Sex-determining transformer protein 2 precursor (Ce-Tra-2) gb|AAA28150.1| membrane protein 0.0
blast Q17307 Sex-determining transformer protein 2 precursor (Cb-Tra-2A) 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021954.1 10 7-29,447-469,476-498,585-607,736-758,922-944,951-973,983-1002,1023-1045,1076-1098
C15F1.3b 10 7-29,447-469,476-498,585-607,736-758,922-944,951-973,983-1002,1023-1045,1076-1098
Sequence
Target Sequence 
>C. ELEGANS:188199_S_AT
gaatttccgaagcgtgaagtaacttgtcaaaattggtggccattcagcagttctcggtca
tcaatttgttcatttttcttccactcacatccgtcatccattattcttgccaccttgtcc
atttttcgccaatatatatatttattctcacttttctctttctctcattcatcttccgac
gtttcaaataagaagtcccgaggttactgtagatgtatgagttgcttttcaatctttttt
caacgtgtgtggcctcaagagcattggaacacggccaacaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTTCCGAAGCGTGAAGTAACTT 98 325 3142 Antisense
AAATTGGTGGCCATTCAGCAGTTCT 116 115 3171 Antisense
GCAGTTCTCGGTCATCAATTTGTTC 221 311 3188 Antisense
CCTTGTCCATTTTTCGCCAATATAT 402 269 3254 Antisense
ATATATTTATTCTCACTTTTCTCTT 652 19 3277 Antisense
CTCATTCATCTTCCGACGTTTCAAA 501 229 3305 Antisense
TAAGAAGTCCCGAGGTTACTGTAGA 622 637 3330 Antisense
GTATGAGTTGCTTTTCAATCTTTTT 596 455 3356 Antisense
ATCTTTTTTCAACGTGTGTGGCCTC 677 31 3373 Antisense
CAACGTGTGTGGCCTCAAGAGCATT 300 187 3382 Antisense
AGAGCATTGGAACACGGCCAACAAT 3 69 3399 Antisense
Sequence Source Affymetrix Proprietary Database