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[188194_at] heparin-binding gf

Description C05D11.4 /REP_DB=WormBase Gene ID /WP=CE24786 /GEN=let-756 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=heparin-binding gf
Cluster 6grp_high1_10_10 > 38      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_total > 27      4grp_total > 22      6grp_cgc > 06      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 179
1 2 3 4 range
A 307.4
(3)
P 		222
(-0.2)
P 		147
(-3)
P 		259.3
(1.2)
P 		160
B 276.9
(1.3)
P 		222.6
(-0.7)
P 		156.4
(-3)
P 		326.2
(3)
P 		170
C 258.7
(2.7)
P 		189.9
(-3)
P 		226.7
(0)
P 		262.4
(3)
P 		73
avr 281
(2.9) 		211.5
(-1) 		176.7
(-3) 		282.6
(3) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 281
1 2 3 4 5 6 range
A 216.2
(3)
P 		89.7
(-1.2)
P 		58.8
(-2.2)
P 		57.4
(-2.3)
A 		36.2
(-3)
P 		140.2
(0.5)
P 		180
B 316.8
(3)
P 		103.8
(-1.7)
P 		47
(-3)
A 		80.9
(-2.2)
P 		65.5
(-2.6)
A 		111.2
(-1.6)
P 		270
C 207.7
(3)
P 		129.3
(-0.2)
P 		64.2
(-2.8)
P 		83.6
(-2)
M 		59
(-3)
A 		112
(-0.9)
P 		149
avr 246.9
(3) 		107.6
(-1.3) 		56.7
(-2.9) 		74
(-2.4) 		53.6
(-3) 		121.1
(-0.9) 		193
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 111.9
(3)
P 		48.2
(-2.2)
P 		38.4
(-3)
P 		38
(-3)
P 		74
B 88.3
(3)
P 		55
(-0.8)
P 		36.2
(-3)
P 		69.5
(0.8)
P 		52
C 85.6
(3)
P 		54.6
(-1.5)
P 		79
(2)
P 		44.2
(-3)
P 		41
avr 95.3
(3) 		52.6
(-2.7) 		51.2
(-2.9) 		50.6
(-3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 39.9
(3)
P 		31.9
(1.3)
P 		12.8
(-2.6)
P 		14.6
(-2.2)
A 		10.9
(-3)
P 		34.1
(1.8)
P 		29
B 110.2
(3)
P 		34.5
(-1.6)
P 		11.4
(-3)
A 		21.4
(-2.4)
P 		26.6
(-2.1)
A 		41.5
(-1.2)
P 		99
C 77
(3)
P 		39.4
(-0.7)
P 		16.3
(-3)
P 		16.8
(-3)
M 		19.1
(-2.7)
A 		37.5
(-0.9)
P 		61
avr 75.7
(3) 		35.3
(-0.9) 		13.5
(-3) 		17.6
(-2.6) 		18.9
(-2.5) 		37.7
(-0.7) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188194_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6366
Sequence Type Exemplar sequence
Representative Public ID C05D11.4 NCBI
Target Description C05D11.4 /REP_DB=WormBase Gene ID /WP=CE24786 /GEN=let-756 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=heparin-binding gf
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066002(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066002 NCBI Caenorhabditis elegans LEThal family member (let-756) (let-756) mRNA, complete cds. 11/11 None
SNAP00000019448 ENSEMBL cdna:SNAP chromosome:CEL140:III:6407074:6409529:1 11/11 None
GENEFINDER00000019463 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6407074:6409529:1 11/11 None
C05D11.4 ENSEMBL cdna:known chromosome:CEL140:III:6407066:6409955:1 gene:C05D11.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6407072-6409528(+) 93.58 100.0
Public Domain and Genome References
Gene Title heparin-binding gf
Gene Symbol let-756
Ensembl C05D11.4
Entrez Gene 175909 Entrez gene
SwissProt Q11184 EMBL-EBI
WormBase CE24786 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.11182.1.S1_AT similar to Fibroblast growth factor-20 (FGF-20) cfa
CHICKEN:GGAAFFX.8705.1.S1_AT similar to Fibroblast growth factor-20 (FGF-20) gga
HG-U133_PLUS_2:220394_AT fibroblast growth factor 20 hs
HG-U133A:220394_AT fibroblast growth factor 20 hs
HG-FOCUS:220394_AT fibroblast growth factor 20 hs
HG-U133A_2:220394_AT fibroblast growth factor 20 hs
U133_X3P:G9789946_3P_AT fibroblast growth factor 20 hs
HG-U95D:81080_AT fibroblast growth factor 20 hs
MOE430A:1421677_AT fibroblast growth factor 20 mm
MOUSE430_2:1421677_AT fibroblast growth factor 20 mm
MOUSE430A_2:1421677_AT fibroblast growth factor 20 mm
RAT230_2:1369606_AT fibroblast growth factor 20 rn
RAE230A:1369606_AT fibroblast growth factor 20 rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8083 growth factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB53825 Lethal protein 756 [Caenorhabditis elegans] ref|NP_498403.1| LEThal family member (let-756) [Caenorhabditis elegans] emb|CAA09234.1| LET-756 protein [Caenorhabditis elegans] sp|Q11184|LE756_CAEEL Let-756 protein (Lethal protein 756) 0.0
blast H88481 protein let-756 [imported] - Caenorhabditis elegans 1.0E-157
blast CAE72484 Hypothetical protein CBG19663 [Caenorhabditis briggsae] 1.0E-133
Protein Domains
Database ID Description E-Value
Pfam IPR002348 EMBL-EBI Interleukin 1/heparin-binding growth factor 1.2E-34
Sequence
Target Sequence 
>C. ELEGANS:188194_AT
aaccgatctggtagttgcttcactctttcaccaacctcccagtcatccattattccgaca
gcagaccgttacaaaaccaccaaatccacatcgtatttcaaatttacgagcaaaagtgga
aatgacgaaccaagctgagaagcaaagattattagaagaaaagaaaagacgaagagaaaa
gaagaaacgaaggagagaagatcgattaagaaaagaggaacaaattagagaagcccgtcg
ccaagagctcaaaagtcttcgtgaagaagaactccgacgtcgttatcaacaacaacagca
gcaacaagcatctactcaaactcggtacaatcgtcctcaaaatccagcgaatccttatcc
aacgtatcgacctcttccaacaagatcaacagtacaatctccacgacctgcatacaatcc
atattggcagtctccagtgacacagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACCGATCTGGTAGTTGCTTCACTC 380 147 690 Antisense
TATTCCGACAGCAGACCGTTACAAA 286 663 740 Antisense
AAACCACCAAATCCACATCGTATTT 424 125 763 Antisense
AAATTAGAGAAGCCCGTCGCCAAGA 161 117 911 Antisense
GAAGAACTCCGACGTCGTTATCAAC 424 339 955 Antisense
CAAACTCGGTACAATCGTCCTCAAA 11 191 1006 Antisense
CGTCCTCAAAATCCAGCGAATCCTT 651 245 1021 Antisense
AATCCTTATCCAACGTATCGACCTC 139 167 1039 Antisense
GATCAACAGTACAATCTCCACGACC 373 421 1073 Antisense
CACGACCTGCATACAATCCATATTG 496 199 1091 Antisense
TATTGGCAGTCTCCAGTGACACAGG 207 665 1111 Antisense
Sequence Source Affymetrix Proprietary Database