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[188189_at]

Description F52B11.6 /REP_DB=WormBase Gene ID /WP=CE18726 /GEN=ins-34 /TR=Q9XUE8 /GB=CAB05196.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 38      4grp_total > 26      6grp_cgc > 07      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 8.2
(-3)
A 		14.8
(1.4)
A 		8.9
(-2.5)
A 		17.3
(3)
A 		9
B 8.6
(-3)
A 		42.4
(3)
A 		9.8
(-2.8)
A 		22.5
(-0.5)
A 		34
C 6.3
(-2.7)
A 		22
(-1.3)
A 		3.6
(-3)
A 		67.3
(3)
A 		64
avr 7.7
(-2.9) 		26.4
(1) 		7.4
(-3) 		35.7
(3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 50.7
(3)
A 		9.8
(-2.7)
A 		11
(-2.6)
A 		8
(-3)
A 		14.8
(-2)
A 		19.8
(-1.3)
A 		43
B 14.4
(-1.1)
A 		13.4
(-1.3)
A 		4.5
(-3)
A 		9.4
(-2.1)
A 		18.6
(-0.3)
A 		36.3
(3)
A 		32
C 25.7
(3)
A 		5.8
(-2.8)
A 		6.9
(-2.4)
A 		5
(-3)
A 		9.4
(-1.7)
A 		11.4
(-1.1)
A 		21
avr 30.3
(3) 		9.7
(-2.4) 		7.5
(-3) 		7.5
(-3) 		14.3
(-1.2) 		22.5
(0.9) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 3
(1.7)
A 		3.2
(3)
A 		2.3
(-3)
A 		2.5
(-1.7)
A 		1
B 2.7
(-2.7)
A 		10.5
(3)
A 		2.3
(-3)
A 		4.8
(-1.2)
A 		8
C 2.1
(-2.5)
A 		6.3
(0)
A 		1.3
(-3)
A 		11.3
(3)
A 		10
avr 2.6
(-2.2) 		6.7
(3) 		2
(-3) 		6.2
(2.4) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 9.3
(3)
A 		3.5
(-1.8)
A 		2.4
(-2.7)
A 		2
(-3)
A 		4.5
(-0.9)
A 		4.8
(-0.7)
A 		7
B 5
(-1.1)
A 		4.5
(-1.4)
A 		1.1
(-3)
A 		2.5
(-2.3)
A 		7.6
(0.1)
A 		13.5
(3)
A 		12
C 9.5
(3)
A 		1.8
(-2.4)
A 		1.7
(-2.5)
A 		1
(-3)
A 		3
(-1.6)
A 		3.8
(-1)
A 		9
avr 7.9
(3) 		3.3
(-1.5) 		1.7
(-3) 		1.8
(-2.9) 		5
(0.2) 		7.4
(2.5) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188189_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9346
Sequence Type Exemplar sequence
Representative Public ID F52B11.6 NCBI
Target Description F52B11.6 /REP_DB=WormBase Gene ID /WP=CE18726 /GEN=ins-34 /TR=Q9XUE8 /GB=CAB05196.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070301(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070301 NCBI Caenorhabditis elegans INSulin related family member (ins-34) (ins-34) mRNA, complete cds. 11/11 None
SNAP00000035584 ENSEMBL cdna:SNAP chromosome:CEL140:IV:14106555:14107628:1 9/11 None
GENEFINDER00000035592 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:14106555:14107628:1 9/11 None
F52B11.6 ENSEMBL cdna:known chromosome:CEL140:IV:14104891:14107628:1 gene:F52B11.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:14104896-14107634(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ins-34
Ensembl F52B11.6
Entrez Gene 191692 Entrez gene
SwissProt Q9XUE8 EMBL-EBI
WormBase CE18726 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05196 Hypothetical protein F52B11.6 [Caenorhabditis elegans] ref|NP_502702.1| INSulin related family member (ins-34) [Caenorhabditis elegans] 3.0E-55
blast CAB05196 Hypothetical protein F52B11.6 [Caenorhabditis elegans] ref|NP_502702.1| INSulin related family member (ins-34) [Caenorhabditis elegans] 5.0E-45
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502702.1 1 20-39
F52B11.6 1 20-39
SNAP00000035584 1 7-25
GENEFINDER00000035592 1 7-25
Sequence
Target Sequence 
>C. ELEGANS:188189_AT
atgcaaattcggtccatcgtgcaccctttacttaccaaaccagagaaaaggggtatcatg
cttcaccacaaaaccctcatcattgctcttctcctgacacttttcatttcgggaatcgac
agtcttccattccgaaagcataacaatcaccgtcacctcaaaaatcaaaaggcccaacaa
ttgaaagaagaagccactgaagctccaactccagccccgactacaaccaaagctccatct
ggatctgctactacaaccaccaccgtgaaaacgactgcagctccattggctcaagtcaat
ccacaatgcttgagacggctcactttgctcgcgagaggagtctgtagacagccgtgccaa
ccatcagataaacca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCAAATTCGGTCCATCGTGCACC 603 43 13 Antisense
GGGGTATCATGCTTCACCACAAAAC 251 493 62 Antisense
TTCTCCTGACACTTTTCATTTCGGG 200 693 101 Antisense
CGGGAATCGACAGTCTTCCATTCCG 93 243 122 Antisense
GAAAGCATAACAATCACCGTCACCT 48 361 146 Antisense
AAGCTCCATCTGGATCTGCTACTAC 319 151 242 Antisense
TGAAAACGACTGCAGCTCCATTGGC 463 571 278 Antisense
GCTCCATTGGCTCAAGTCAATCCAC 538 297 292 Antisense
GCTCACTTTGCTCGCGAGAGGAGTC 73 301 330 Antisense
GAGGAGTCTGTAGACAGCCGTGCCA 343 403 347 Antisense
GCCGTGCCAACCATCAGATAAACCA 465 283 363 Antisense
Sequence Source Affymetrix Proprietary Database