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[188183_at]

Description T08G11.2 /REP_DB=WormBase Gene ID /WP=CE13445 /GEN=egl-32 /TR=Q94011 /GB=CAB02305.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 11      6grp_cgc > 79      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 31
(-3)
A 		50.5
(0.7)
P 		35.4
(-2.2)
A 		62.8
(3)
A 		32
B 38
(0.5)
A 		23.6
(-1.1)
A 		6.6
(-3)
A 		59.9
(3)
P 		53
C 18.5
(-3)
A 		28
(-2.2)
A 		19.8
(-2.9)
A 		91.5
(3)
P 		73
avr 29.2
(-2) 		34
(-1.4) 		20.6
(-3) 		71.4
(3) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 38.7
(-2.6)
A 		34.1
(-2.8)
P 		31.3
(-3)
P 		42.8
(-2.4)
P 		117.1
(1.7)
P 		140.6
(3)
P 		109
B 28.6
(-2.2)
A 		30.2
(-2.1)
A 		13.8
(-3)
A 		56.9
(-0.6)
A 		107.7
(2.3)
P 		119.6
(3)
P 		106
C 31.7
(-1.9)
A 		24.2
(-2.2)
A 		8.2
(-3)
A 		36.6
(-1.6)
A 		104.5
(1.7)
P 		131.5
(3)
P 		123
avr 33
(-2.2) 		29.5
(-2.4) 		17.8
(-3) 		45.4
(-1.5) 		109.8
(1.9) 		130.6
(3) 		113
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 11.3
(3)
A 		11
(2.1)
P 		9.3
(-2.7)
A 		9.2
(-3)
A 		2
B 12.1
(2.6)
A 		5.8
(-0.7)
A 		1.5
(-3)
A 		12.8
(3)
P 		11
C 6.1
(-3)
A 		8.1
(-1.7)
A 		6.9
(-2.5)
A 		15.4
(3)
P 		9
avr 9.8
(0.5) 		8.3
(-0.8) 		5.9
(-3) 		12.5
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 7.1
(-2.9)
A 		12.1
(-1.9)
P 		6.8
(-3)
P 		10.9
(-2.1)
P 		35.3
(3)
P 		34.2
(2.8)
P 		29
B 10
(-2)
A 		10.1
(-2)
A 		3.4
(-3)
A 		15.1
(-1.3)
A 		43.7
(2.9)
P 		44.6
(3)
P 		41
C 11.7
(-1.6)
A 		7.4
(-2.2)
A 		2.1
(-3)
A 		7.4
(-2.2)
A 		33.8
(1.5)
P 		44
(3)
P 		42
avr 9.6
(-2.1) 		9.9
(-2.1) 		4.1
(-3) 		11.1
(-1.9) 		37.6
(2.5) 		40.9
(3) 		37
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188183_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.936
Sequence Type Exemplar sequence
Representative Public ID T08G11.2 NCBI
Target Description T08G11.2 /REP_DB=WormBase Gene ID /WP=CE13445 /GEN=egl-32 /TR=Q94011 /GB=CAB02305.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060001(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060001 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-32) (egl-32) mRNA, complete cds. 11/11 None
SNAP00000035562 ENSEMBL cdna:SNAP chromosome:CEL140:I:8927514:8928590:-1 11/11 None
GENEFINDER00000035567 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8927514:8928590:-1 11/11 None
T08G11.2 ENSEMBL cdna:known chromosome:CEL140:I:8927514:8928590:-1 gene:T08G11.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8927444-8928521(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol egl-32
Ensembl T08G11.2
Entrez Gene 191630 Entrez gene
SwissProt Q94011 EMBL-EBI
WormBase CE13445 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02305 Hypothetical protein T08G11.2 [Caenorhabditis elegans] ref|NP_492402.1| EGg Laying defective family member (egl-32) [Caenorhabditis elegans] 1.0E-165
blast AAB52464 Hypothetical protein B0207.11 [Caenorhabditis elegans] ref|NP_491721.1| B0207.11 [Caenorhabditis elegans] 1.0E-100
blast CAE60431 Hypothetical protein CBG04039 [Caenorhabditis briggsae] 8.0E-96
Sequence
Target Sequence 
>C. ELEGANS:188183_AT
ctccagttttggttgatgacagtcagttgtatgttatgattggaccaccgagtgaacatc
caggggaaccacctgcatcagagccaactgaaacgtataaaaccaacacaatcgatgaat
caacattcaaaggaagtgaagcattgtcagcttacattggggttgtaacacttgccaacg
cagaaaaccatttgactcgccgtggtgaatttgctctctatcacttgttcaatccaaatg
gacgtctggacacaattactgacaagctaccattaatgattgtctaccgtaccactacca
aaaagaatcgtcactactcgatccgtactacttgtgaccaacaattctttgtcgactgtg
gatatcctaatgttcgaaaacattattcgctcagtcatttggtcatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCCAGTTTTGGTTGATGACAGTCA 36 235 377 Antisense
GACCACCGAGTGAACATCCAGGGGA 423 383 419 Antisense
CATCCAGGGGAACCACCTGCATCAG 553 209 433 Antisense
GCATTGTCAGCTTACATTGGGGTTG 580 307 517 Antisense
GGGGTTGTAACACTTGCCAACGCAG 571 497 535 Antisense
CGCAGAAAACCATTTGACTCGCCGT 53 253 555 Antisense
TCGCCGTGGTGAATTTGCTCTCTAT 429 599 573 Antisense
TGCTCTCTATCACTTGTTCAATCCA 444 581 588 Antisense
GGACACAATTACTGACAAGCTACCA 256 527 624 Antisense
GCTACCATTAATGATTGTCTACCGT 675 301 642 Antisense
TTATTCGCTCAGTCATTTGGTCATG 214 679 759 Antisense
Sequence Source Affymetrix Proprietary Database