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[188180_at]

Description F49E10.3 /REP_DB=WormBase Gene ID /WP=CE07264 /GEN=flp-7 /TR=Q20598 /GB=AAC69107.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 13      6grp_high1_10_10 > 20      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 22      6grp_total > 33      4grp_total > 13      6grp_cgc > 71      6grp_cgc_sub > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3026
1 2 3 4 range
A 2362.1
(-0.2)
P 		1682.9
(-1.5)
P 		941.5
(-3)
P 		3967.4
(3)
P 		3026
B 2556.4
(0.1)
P 		1301.9
(-2.5)
P 		1041.2
(-3)
P 		3944.2
(3)
P 		2903
C 2568.9
(0.9)
P 		1277.8
(-2.2)
P 		967.4
(-3)
P 		3421.5
(3)
P 		2454
avr 2495.8
(0.2) 		1420.9
(-2.1) 		983.4
(-3) 		3777.7
(3) 		2794

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1135
1 2 3 4 5 6 range
A 2203.3
(3)
P 		1470.4
(-1.6)
P 		1873.1
(0.9)
P 		1482.2
(-1.5)
P 		1347.1
(-2.4)
P 		1246.8
(-3)
P 		957
B 1747.4
(-0.3)
P 		1644.8
(-0.9)
P 		2349.3
(3)
P 		1619.9
(-1)
P 		1257.4
(-3)
P 		1376.1
(-2.3)
P 		1092
C 1562.3
(0.5)
P 		1804.6
(3)
P 		1548.4
(0.4)
P 		1712.7
(2.1)
P 		1214.4
(-3)
P 		1452.2
(-0.6)
P 		590
avr 1837.7
(2.2) 		1639.9
(0.4) 		1923.6
(3) 		1604.9
(0.1) 		1273
(-3) 		1358.4
(-2.2) 		651
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 619
1 2 3 4 range
A 860.1
(3)
P 		365.3
(-1.8)
P 		246.1
(-3)
P 		580.8
(0.3)
P 		614
B 815.2
(2.7)
P 		321.6
(-2.2)
P 		241.3
(-3)
P 		840.5
(3)
P 		599
C 850.4
(3)
P 		367.4
(-2.6)
P 		337.2
(-3)
P 		576.9
(-0.2)
P 		513
avr 841.9
(3) 		351.4
(-2.2) 		274.9
(-3) 		666.1
(1.1) 		567

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 304
1 2 3 4 5 6 range
A 406.2
(-0.2)
P 		523
(3)
P 		407.9
(-0.1)
P 		375.9
(-1)
P 		406.5
(-0.2)
P 		303.6
(-3)
P 		219
B 608
(3)
P 		547.5
(1)
P 		570.5
(1.7)
P 		429.1
(-3)
P 		510.8
(-0.3)
P 		513.1
(-0.2)
P 		179
C 579.1
(3)
P 		550.7
(2.3)
P 		394.1
(-1.7)
P 		344.4
(-3)
P 		393
(-1.8)
P 		486.1
(0.6)
P 		235
avr 531.1
(2.6) 		540.4
(3) 		457.5
(-0.2) 		383.1
(-3) 		436.8
(-1) 		434.3
(-1) 		157
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188180_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16319
Sequence Type Exemplar sequence
Representative Public ID F49E10.3 NCBI
Target Description F49E10.3 /REP_DB=WormBase Gene ID /WP=CE07264 /GEN=flp-7 /TR=Q20598 /GB=AAC69107.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076584(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076584 NCBI Caenorhabditis elegans FMRF-Like Peptide family member (flp-7) (flp-7) mRNA, complete cds. 11/11 None
SNAP00000004446 ENSEMBL cdna:SNAP chromosome:CEL140:X:5885825:5886573:1 11/11 None
GENEFINDER00000004449 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5885825:5886573:1 11/11 None
F49E10.3.2 ENSEMBL cdna:known chromosome:CEL140:X:5885821:5886781:1 gene:F49E10.3 11/11 None
F49E10.3.1 ENSEMBL cdna:known chromosome:CEL140:X:5885823:5886696:1 gene:F49E10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5885824-5886573(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol flp-7
Ensembl F49E10.3
Entrez Gene 180854 Entrez gene
SwissProt Q20598 EMBL-EBI
WormBase CE07264 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7218 neuropeptide signaling pathway inferred from electronic annotation QuickGO AmiGO
7218 neuropeptide signaling pathway inferred from sequence similarity QuickGO AmiGO
19932 second-messenger-mediated signaling inferred from direct assay QuickGO AmiGO
40013 negative regulation of locomotion inferred from direct assay QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC69107 Fmrf-like peptide protein 7 [Caenorhabditis elegans] ref|NP_508985.1| FMRF-Like Peptide family member (flp-7) [Caenorhabditis elegans] gb|AAC08944.1| FMRFamide-like peptide 7 [Caenorhabditis elegans] 9.0E-85
blast CAE68815 Hypothetical protein CBG14771 [Caenorhabditis briggsae] 2.0E-62
Protein Domains
Database ID Description E-Value
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 2.5
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 3.9
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 3.9
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 3.9
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 4.2
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 2.2
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 3.9
Sequence
Target Sequence 
>C. ELEGANS:188180_AT
tgctcttggtctcctagttttggtattggccgaggaatcagccgaacaacaagttcaaga
accaactgaactcgagaagtcaggagagcaactctcagaggaggacttaattgacgagca
aaagcgtactccaatgcaacgctcatcaatggttcggttcggacggtcaccaatgcaacg
ctcatccatggttagattcggaaagcgttcgccaatgcaaaggtcctcaatggttcgctt
tggaaaacggtcgccaatgcaacgatcttcaatggtgcgttttggaaaacgttcaccgat
ggagcgttcggctatggttcgctttggaagatcacctatggaccgatcgaaaatggtccg
atttggtagatcatcaattgatcgcgcttctatggttcgactcggaaaacggacaccaat
gcagagatcatcgatggtccgttttggcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTCTTGGTCTCCTAGTTTTGGTA 424 579 39 Antisense
CGTACTCCAATGCAACGCTCATCAA 457 247 163 Antisense
TCAATGGTTCGGTTCGGACGGTCAC 630 627 184 Antisense
AATGCAACGCTCATCCATGGTTAGA 469 165 210 Antisense
AGCGTTCGCCAATGCAAAGGTCCTC 639 81 242 Antisense
AAGGTCCTCAATGGTTCGCTTTGGA 45 161 258 Antisense
AACGGTCGCCAATGCAACGATCTTC 452 143 284 Antisense
AACGTTCACCGATGGAGCGTTCGGC 706 141 326 Antisense
GTTCGGCTATGGTTCGCTTTGGAAG 287 441 344 Antisense
TGATCGCGCTTCTATGGTTCGACTC 523 565 417 Antisense
GATCATCGATGGTCCGTTTTGGCAA 72 417 464 Antisense
Sequence Source Affymetrix Proprietary Database