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[188176_s_at]

Description R13H8.1 /REP_DB=WormBase Gene ID /WP=CE12818 /GEN=daf-16 /TR=O44756 /GB=AAB96741.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 93      6grp_high1_10_10 > 48      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 85      6grp_total > 49      4grp_total > 03      6grp_highest_sub > Cu      6grp_cgc > 18      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2162
1 2 3 4 range
A 796
(-2.3)
P 		669.4
(-2.6)
P 		532.3
(-3)
P 		2694.1
(3)
P 		2162
B 766.4
(-2.4)
P 		589.4
(-2.9)
P 		559.8
(-3)
P 		2683.8
(3)
P 		2124
C 855.7
(-1.8)
P 		816.5
(-2)
P 		586
(-3)
P 		1924.4
(3)
P 		1338
avr 806
(-2.2) 		691.8
(-2.6) 		559.4
(-3) 		2434.1
(3) 		1875

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 829
1 2 3 4 5 6 range
A 813.4
(-0.4)
P 		706.5
(-1.2)
P 		461.8
(-3)
P 		631.6
(-1.8)
P 		667.6
(-1.5)
P 		1282.2
(3)
P 		820
B 734.6
(1.8)
P 		680.4
(0.8)
P 		453.6
(-3)
P 		534.6
(-1.6)
P 		608.2
(-0.4)
P 		807.3
(3)
P 		354
C 1250.6
(3)
P 		676.2
(-2.2)
P 		718.5
(-1.8)
P 		583.2
(-3)
P 		829.5
(-0.8)
P 		936.8
(0.2)
P 		667
avr 932.9
(2) 		687.7
(-1.2) 		544.6
(-3) 		583.1
(-2.5) 		701.8
(-1) 		1008.8
(3) 		464
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 442
1 2 3 4 range
A 289.8
(0.5)
P 		145.3
(-2.9)
P 		139.1
(-3)
P 		394.4
(3)
P 		255
B 244.4
(-1.4)
P 		145.6
(-2.8)
P 		129.7
(-3)
P 		571.9
(3)
P 		442
C 283.3
(0.9)
P 		234.8
(-1.5)
P 		204.2
(-3)
P 		324.5
(3)
P 		120
avr 272.5
(-0.5) 		175.2
(-2.6) 		157.7
(-3) 		430.3
(3) 		273

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 363
1 2 3 4 5 6 range
A 149.9
(-1.6)
P 		251.3
(1.3)
P 		100.6
(-3)
P 		160.2
(-1.3)
P 		201.4
(-0.1)
P 		312.2
(3)
P 		212
B 255.6
(1.6)
P 		226.5
(0.7)
P 		110.2
(-3)
P 		141.6
(-2)
P 		247.1
(1.3)
P 		301.1
(3)
P 		191
C 463.5
(3)
P 		206.3
(-1.5)
P 		182.9
(-1.9)
P 		117.3
(-3)
P 		268.4
(-0.4)
P 		313.6
(0.4)
P 		346
avr 289.7
(2.3) 		228
(0.3) 		131.2
(-3) 		139.7
(-2.7) 		239
(0.6) 		309
(3) 		178
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188176_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.387
Sequence Type Exemplar sequence
Representative Public ID R13H8.1 NCBI
Target Description R13H8.1 /REP_DB=WormBase Gene ID /WP=CE12818 /GEN=daf-16 /TR=O44756 /GB=AAB96741.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026427(11), NM_001026425(10), NM_001026424(11), NM_001026423(11), NM_001026426(10), NM_001026422(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026427 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 11/11 None
NM_001026425 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 10/11 None
NM_001026424 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 11/11 None
NM_001026423 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 11/11 None
NM_001026426 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 10/11 None
NM_001026422 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 11/11 None
R13H8.1d.2 ENSEMBL cdna:known chromosome:CEL140:I:10759265:10783171:1 gene:R13H8.1 10/11 None
R13H8.1b ENSEMBL cdna:known chromosome:CEL140:I:10770891:10784824:1 gene:R13H8.1 11/11 A 
R13H8.1c ENSEMBL cdna:known chromosome:CEL140:I:10770891:10784821:1 gene:R13H8.1 11/11 A 
R13H8.1d.1 ENSEMBL cdna:known chromosome:CEL140:I:10771709:10783171:1 gene:R13H8.1 10/11 None
R13H8.1e.2 ENSEMBL cdna:known chromosome:CEL140:I:10773329:10783171:1 gene:R13H8.1 10/11 None
R13H8.1a ENSEMBL cdna:known chromosome:CEL140:I:10779578:10784583:1 gene:R13H8.1 11/11 A 
R13H8.1e.1 ENSEMBL cdna:known chromosome:CEL140:I:10780543:10783171:1 gene:R13H8.1 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019257 5/11 Cross Hyb Matching Probes None
GENEFINDER00000019269 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10779559-10783737(+) 98.61 100.0
Public Domain and Genome References
Ensembl R13H8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001021598 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] 0.0
blast NP_001021596 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] 0.0
blast NP_001021595 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] gb|AAK82919.1| Abnormal dauer formation protein 16, isoform c [Caenorhabditis elegans] gb|AAC47803.1| DAF-16 [Caenorhabditis elegans] gb|AAB84390.1| fork head-related transcription factor DAF-16a1 [Caenorhabditis elegans] 0.0
blast NP_001021594 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] gb|AAK82918.1| Abnormal dauer formation protein 16, isoform b [Caenorhabditis elegans] gb|AAB84391.1| fork head-related transcription factor DAF-16a2 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 8.968310171678829E-44
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 2.00385680398449E-43
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 2.99877871365511E-43
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 9.99999991097579E-38
Pfam IPR001766 EMBL-EBI Fork head transcription factor 5.4E-22
Pfam IPR001766 EMBL-EBI Fork head transcription factor 5.4E-22
Pfam IPR001766 EMBL-EBI Fork head transcription factor 8.7E-23
Pfam IPR001766 EMBL-EBI Fork head transcription factor 6.2E-23
Pfam IPR001766 EMBL-EBI Fork head transcription factor 9.1E-17
Pfam IPR001766 EMBL-EBI Fork head transcription factor 5.4E-22
Sequence
Target Sequence 
>C. ELEGANS:188176_S_AT
caccaccatcataccacgagttgaacagtgtccgtggatcgtgtgctcagaatccacttc
ttcgaaatccaattgtgccaagcactaacttcaagccaatgccactaccgggtgcctatg
gaaactatcaaaatggtggaataactccaatcaattggctatcaacatccaactcatctc
cactgcctggaattcaatcgtgtggaattgtagctgcacagcatactgtcgcttcttcat
cggctcttccaattgatttggaaaatctgacacttcccgatcagccactgatggatacta
tggatgttgatgcattgatcagacatgagctgagtcaagctggagggcagcatattcatt
ttgatttaaataatcgattttctggaaaatttcaaaaaaaaatcggaatttttaatagta
aaatcattagcactgaaaattatttgaaaaaaaacttcaaatacaaattttgttttcgaa
aaaaaaaatttaaatatatattttcagaaatcttccgtcttcatcttttcaaatccctac
ctacacacactcaacgatcatcacag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACCACCATCATACCACGAGTTGAA 178 203 1286 Antisense
AACAGTGTCCGTGGATCGTGTGCTC 152 137 1309 Antisense
TGTGCTCAGAATCCACTTCTTCGAA 324 555 1327 Antisense
TGTGCCAAGCACTAACTTCAAGCCA 128 557 1359 Antisense
TAACTTCAAGCCAATGCCACTACCG 586 639 1371 Antisense
GCCACTACCGGGTGCCTATGGAAAC 522 279 1386 Antisense
TGGCTATCAACATCCAACTCATCTC 152 545 1441 Antisense
CTCATCTCCACTGCCTGGAATTCAA 580 229 1458 Antisense
TGCACAGCATACTGTCGCTTCTTCA 629 577 1500 Antisense
GAAAATCTGACACTTCCCGATCAGC 535 353 1546 Antisense
TACACACACTCAACGATCATCACAG 118 643 1827 Antisense
Sequence Source Affymetrix Proprietary Database