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[188164_at]

Description H26D21.1 /REP_DB=WormBase Gene ID /WP=CE20999 /GEN=hus-1 /TR=Q9XYP0 /GB=AAC78225.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 98      4grp_high_8_8 > 06      6grp_highest_nonorm > 94      6grp_total > 97      4grp_total > 02      6grp_highest_sub > In      6grp_cgc > 69      6grp_high1_sub > In      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 444
1 2 3 4 range
A 384.3
(-2.5)
P 		391.6
(-2.3)
A 		358.7
(-3)
A 		647.4
(3)
P 		289
B 335.4
(-1.8)
P 		270.9
(-3)
P 		301.1
(-2.4)
P 		599.7
(3)
P 		329
C 376.1
(-3)
P 		408.4
(-2.4)
P 		402.1
(-2.5)
P 		715
(3)
P 		339
avr 365.3
(-2.8) 		357
(-2.9) 		354
(-3) 		654
(3) 		300

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 773
1 2 3 4 5 6 range
A 410.5
(-3)
P 		500.9
(-1.7)
P 		452.5
(-2.4)
P 		638.4
(0.2)
P 		774.8
(2.1)
P 		838.7
(3)
P 		428
B 374.3
(-2.2)
P 		272.4
(-3)
P 		381.1
(-2.2)
P 		440.7
(-1.7)
P 		852.8
(1.5)
P 		1045.2
(3)
P 		773
C 373.7
(-3)
P 		478.8
(-1.9)
P 		514.2
(-1.6)
P 		471.8
(-2)
P 		719.5
(0.5)
P 		961.3
(3)
P 		588
avr 386.2
(-3) 		417.4
(-2.7) 		449.3
(-2.3) 		517
(-1.6) 		782.4
(1.2) 		948.4
(3) 		562
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 139.9
(3)
P 		85
(-3)
A 		93.8
(-2)
A 		94.8
(-1.9)
P 		55
B 107
(1)
P 		66.9
(-3)
P 		69.8
(-2.7)
P 		127.8
(3)
P 		61
C 124.5
(-1.1)
P 		117.4
(-3)
P 		140.1
(3)
P 		120.6
(-2.2)
P 		23
avr 123.8
(3) 		89.8
(-3) 		101.2
(-1) 		114.4
(1.3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 314
1 2 3 4 5 6 range
A 75.7
(-3)
P 		178.2
(0.9)
P 		98.5
(-2.1)
P 		161.9
(0.3)
P 		233.8
(3)
P 		204.2
(1.9)
P 		158
B 130.2
(-2.2)
P 		90.7
(-3)
P 		92.5
(-3)
P 		116.7
(-2.5)
P 		346.5
(2.1)
P 		389.7
(3)
P 		299
C 138.5
(-1.8)
P 		146.1
(-1.6)
P 		130.9
(-2)
P 		94.9
(-3)
P 		232.8
(0.6)
P 		321.8
(3)
P 		227
avr 114.8
(-2.8) 		138.3
(-2.1) 		107.3
(-3) 		124.5
(-2.5) 		271
(2) 		305.2
(3) 		198
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188164_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.276
Sequence Type Exemplar sequence
Representative Public ID H26D21.1 NCBI
Target Description H26D21.1 /REP_DB=WormBase Gene ID /WP=CE20999 /GEN=hus-1 /TR=Q9XYP0 /GB=AAC78225.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058802(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058802 NCBI Caenorhabditis elegans human HUS1 related family member (hus-1) (hus-1) mRNA, complete cds. 11/11 None
SNAP00000020760 ENSEMBL cdna:SNAP chromosome:CEL140:I:3698114:3699224:1 11/11 None
H26D21.1 ENSEMBL cdna:known chromosome:CEL140:I:3698114:3699306:1 gene:H26D21.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000020761 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3698113-3699224(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hus-1
Ensembl H26D21.1
Entrez Gene 171939 Entrez gene
SwissProt Q9XYP0 EMBL-EBI
WormBase CE20999 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1584057_AT similar to HUS1 checkpoint protein cfa
CANINE_2:CFA.11015.1.A1_AT similar to HUS1 checkpoint protein cfa
DROSOPHILA_2:1627856_AT dm
DROSGENOME1:144144_AT dm
CHICKEN:GGAAFFX.23916.1.S1_AT similar to Hus1 protein gga
HG-U133_PLUS_2:204884_S_AT HUS1 checkpoint homolog (S. pombe) hs
HG-U133A:204884_S_AT HUS1 checkpoint homolog (S. pombe) hs
HG-FOCUS:204884_S_AT HUS1 checkpoint homolog (S. pombe) hs
HG-U133A_2:204884_S_AT HUS1 checkpoint homolog (S. pombe) hs
U133_X3P:G4758575_3P_A_AT HUS1 checkpoint homolog (S. pombe) hs
U133_X3P:HS.152983.0.S1_3P_A_AT HUS1 checkpoint homolog (S. pombe) hs
U133_X3P:204884_3P_S_AT HUS1 checkpoint homolog (S. pombe) hs
HG-U133A_2:204883_S_AT HUS1 checkpoint homolog (S. pombe) hs
HG-U133_PLUS_2:204883_S_AT HUS1 checkpoint homolog (S. pombe) hs
HG-U133A:204883_S_AT HUS1 checkpoint homolog (S. pombe) hs
HG-U133A_2:217618_X_AT HUS1 checkpoint homolog (S. pombe) hs
HG-U133_PLUS_2:217618_X_AT HUS1 checkpoint homolog (S. pombe) hs
HG-U133A:217618_X_AT HUS1 checkpoint homolog (S. pombe) hs
HG-U95AV2:38153_AT HUS1 checkpoint homolog (S. pombe) hs
HG-U95E:80446_AT HUS1 checkpoint homolog (S. pombe) hs
U133_X3P:HS.233299.0.A1_3P_X_AT HUS1 checkpoint homolog (S. pombe) hs
HG-U95E:80356_R_AT HUS1 checkpoint homolog (S. pombe) hs
U133_X3P:217618_3P_X_AT HUS1 checkpoint homolog (S. pombe) hs
MG-U74AV2:92503_AT Hus1 homolog (S. pombe) mm
MG-U74AV2:92504_AT Hus1 homolog (S. pombe) mm
MG-U74AV2:92505_G_AT Hus1 homolog (S. pombe) mm
MOE430A:1425366_A_AT Hus1 homolog (S. pombe) mm
MOUSE430A_2:1425366_A_AT Hus1 homolog (S. pombe) mm
MOUSE430_2:1425366_A_AT Hus1 homolog (S. pombe) mm
MOE430A:1418308_AT Hus1 homolog (S. pombe) mm
MOUSE430A_2:1418308_AT Hus1 homolog (S. pombe) mm
MOUSE430_2:1418308_AT Hus1 homolog (S. pombe) mm
MU19KSUBA:TC18027_AT Hus1 homolog (S. pombe) (Hus1), mRNA mm
MU19KSUBB:TC28369_AT Hus1 homolog (S. pombe) (Hus1), mRNA mm
RAE230A:1376238_AT similar to hus1+-like rn
RAT230_2:1376238_AT similar to hus1+-like rn
Protein Similarities
Method ID Description E-Value
blast AAC78225 Human hus1 related protein 1 [Caenorhabditis elegans] ref|NP_491203.1| human HUS1 related family member (hus-1) [Caenorhabditis elegans] gb|AAD22029.1| Hus1-like protein [Caenorhabditis elegans] 1.0E-147
blast AAC78225 Human hus1 related protein 1 [Caenorhabditis elegans] ref|NP_491203.1| human HUS1 related family member (hus-1) [Caenorhabditis elegans] gb|AAD22029.1| Hus1-like protein [Caenorhabditis elegans] 1.0E-124
blast CAE66682 Hypothetical protein CBG12021 [Caenorhabditis briggsae] 1.0E-118
blast CAE66682 Hypothetical protein CBG12021 [Caenorhabditis briggsae] 1.0E-106
blast XP_573658 PREDICTED: similar to hus1+-like [Rattus norvegicus] 2.0E-23
blast XP_573658 PREDICTED: similar to hus1+-like [Rattus norvegicus] 5.0E-15
Protein Domains
Database ID Description E-Value
Pfam IPR007150 EMBL-EBI Hus1-like protein 1.3E-6
Pfam IPR007150 EMBL-EBI Hus1-like protein 1.0E-126
Pfam IPR007150 EMBL-EBI Hus1-like protein 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188164_AT
cgatagtttagtacgtgttctgcccggcggacactgttatctgaaactgaaactatcgaa
aagtcaaaaagatgagccaatgttgtctgtcgaagttagaaatccagaagccgatattgt
ttctcatcaaattccaatcacaattattctatcaaaatattggaattcgtactccaggcc
gtcaattggacacagaaaaatgtcaatcagcatgccacctcctaaaatgatgtcccgttt
cttacacgtcttcaaaaatatgaacactcgagttgtgaagctcacagccagtacatcagg
agatttgaggatttctacgaaaattgaaactggcgagattgatgtaagcttctcggatct
tcaaactaatccatcggaaaccgatagtcaagaggatactgcaaaagttcaattaatgat
tcgaaatatttctccattgtttcaaagtttcgccaacactcggacacgtgccaaaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGATAGTTTAGTACGTGTTCTGCCC 183 249 282 Antisense
GAGCCAATGTTGTCTGTCGAAGTTA 222 385 355 Antisense
AGAAGCCGATATTGTTTCTCATCAA 100 75 387 Antisense
TATTGGAATTCGTACTCCAGGCCGT 522 651 439 Antisense
GTACTCCAGGCCGTCAATTGGACAC 436 459 450 Antisense
TGCCACCTCCTAAAATGATGTCCCG 189 587 494 Antisense
GAAGCTCACAGCCAGTACATCAGGA 205 343 558 Antisense
GTAAGCTTCTCGGATCTTCAAACTA 210 463 625 Antisense
AAATATTTCTCCATTGTTTCAAAGT 44 117 705 Antisense
TTCAAAGTTTCGCCAACACTCGGAC 219 677 722 Antisense
CCAACACTCGGACACGTGCCAAAAT 703 275 734 Antisense
Sequence Source Affymetrix Proprietary Database