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[188160_at] calpain

Description T11A5.6 /REP_DB=WormBase Gene ID /WP=CE28256 /TR=Q22386 /GB=CAA96686.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=calpain
Cluster 6grp_total > 06      4grp_total > 30      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 192.3
(3)
M
98.6
(-1.4)
A
64
(-3)
A
112.5
(-0.7)
A
128
B 184.3
(3)
M
94
(-2)
A
76.7
(-3)
A
147.5
(0.9)
A
108
C 191.8
(3)
P
94.7
(-1)
A
47.8
(-3)
A
117.7
(-0.1)
P
144
avr 189.5
(3)
95.8
(-1.4)
62.8
(-3)
125.9
(-0)
127

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 182
1 2 3 4 5 6 range
A 302.7
(3)
A
236
(0.4)
P
149.8
(-3)
A
172.9
(-2.1)
P
195.6
(-1.2)
P
161.3
(-2.5)
A
153
B 196.3
(3)
P
192
(2.7)
A
120.9
(-3)
A
166
(0.6)
A
163.2
(0.4)
M
151.2
(-0.6)
A
75
C 214.8
(3)
A
170
(0)
M
200.8
(2.1)
A
181.4
(0.8)
A
124.4
(-3)
P
151.6
(-1.2)
P
90
avr 237.9
(3)
199.3
(0.2)
157.2
(-2.8)
173.4
(-1.7)
161.1
(-2.5)
154.7
(-3)
83
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 70
(3)
M
21.4
(-2.5)
A
16.7
(-3)
A
16.5
(-3)
A
54
B 58.8
(3)
M
23.2
(-2.2)
A
17.8
(-3)
A
31.4
(-1)
A
41
C 63.5
(3)
P
27.2
(-1.7)
A
16.7
(-3)
A
19.9
(-2.6)
P
47
avr 64.1
(3)
23.9
(-2.1)
17.1
(-3)
22.6
(-2.3)
47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 55.8
(-0.3)
A
83.9
(3)
P
32.6
(-3)
A
43.8
(-1.7)
P
59
(0.1)
P
39.3
(-2.2)
A
51
B 68.3
(3)
P
63.9
(2.3)
A
29.4
(-3)
A
44
(-0.7)
A
66.3
(2.7)
M
56.4
(1.2)
A
39
C 79.6
(3)
A
51.9
(-0.9)
M
51.1
(-1)
A
36.5
(-3)
A
40.2
(-2.5)
P
50.7
(-1)
P
43
avr 67.9
(3)
66.6
(2.7)
37.7
(-3)
41.4
(-2.3)
55.2
(0.5)
48.8
(-0.8)
30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188160_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12516
Sequence Type Exemplar sequence
Representative Public ID T11A5.6 NCBI
Target Description T11A5.6 /REP_DB=WormBase Gene ID /WP=CE28256 /TR=Q22386 /GB=CAA96686.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=calpain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171518(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_171518 NCBI Caenorhabditis elegans T11A5.6 (calpain) mRNA, complete cds. 11/11 None
GENEFINDER00000037724 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9884774:9886938:-1 10/11 None
T11A5.6 ENSEMBL cdna:known chromosome:CEL140:V:9884858:9888542:-1 gene:T11A5.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9884857-9887707(-) 100.0 100.0
Public Domain and Genome References
Gene Title calpain
Ensembl T11A5.6
Entrez Gene 266916 Entrez gene
SwissProt Q22386 EMBL-EBI
WormBase CE28256 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.30081.1.S1_AT similar to small optic lobes cfa
DROSOPHILA_2:1634504_A_AT small optic lobes dm
DROSGENOME1:143360_AT small optic lobes dm
CHICKEN:GGA.7945.1.S1_AT similar to Solh protein gga
CHICKEN:GGAAFFX.25359.1.S1_AT similar to Solh protein gga
HU35KSUBC:RC_AA436605_AT small optic lobes homolog (Drosophila) hs
U133_X3P:HS.55836.0.S2_3P_AT small optic lobes homolog (Drosophila) hs
U133_X3P:HS.121293.0.A1_3P_X_AT small optic lobes homolog (Drosophila) hs
U133_X3P:HS.121293.0.A1_3P_AT small optic lobes homolog (Drosophila) hs
HG-U133A_2:204275_AT small optic lobes homolog (Drosophila) hs
HG-FOCUS:204275_AT small optic lobes homolog (Drosophila) hs
HG-U133_PLUS_2:204275_AT small optic lobes homolog (Drosophila) hs
HG-U133A:204275_AT small optic lobes homolog (Drosophila) hs
HG-U133_PLUS_2:230295_AT small optic lobes homolog (Drosophila) hs
HG-U133B:230295_AT small optic lobes homolog (Drosophila) hs
HG-U95AV2:36485_AT small optic lobes homolog (Drosophila) hs
HG-U95D:74987_F_AT small optic lobes homolog (Drosophila) hs
HG-U95E:81569_AT small optic lobes homolog (Drosophila) hs
MOUSE430A_2:1450817_AT small optic lobes homolog (Drosophila) mm
MOUSE430_2:1450817_AT small optic lobes homolog (Drosophila) mm
MOE430A:1450817_AT small optic lobes homolog (Drosophila) mm
MU19KSUBA:TC22159_AT Small optic lobes homolog (Drosophila) (Solh), mRNA mm
MU19KSUBC:TC41909_AT Small optic lobes homolog (Drosophila) (Solh), mRNA mm
RAE230A:1389792_AT Small optic lobes homolog (Drosophila) (predicted) rn
RAT230_2:1389792_AT Small optic lobes homolog (Drosophila) (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4198 calpain activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC42267 Hypothetical protein T11A5.6 [Caenorhabditis elegans] emb|CAA96686.2| Hypothetical protein T11A5.6 [Caenorhabditis elegans] ref|NP_741604.1| T11A5.6 [Caenorhabditis elegans] 0.0
blast CAE72273 Hypothetical protein CBG19393 [Caenorhabditis briggsae] 0.0
blast AAC71148 Hypothetical protein W05G11.4 [Caenorhabditis elegans] ref|NP_497130.1| W05G11.4 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 3.0E-55
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 3.0E-55
Sequence
Target Sequence
>C. ELEGANS:188160_AT
aatgtcttctcggatctctgataaccacttcacaatcgacgaattttttttggctccagg
agagtatcaaattgtatgtcacagtcaaagctccctactgacaaataaaaaaggagtagt
gaatatggttgtgcacacacgttatccaattttcggagaatatattccaatgtcaccacg
aacgagaatggaatcattgcatcatgtaataattaaagaaggagacattgtcaagaacac
aaacgatggggttgtcatcagaactttgacgagaaaattcagaggaatgatcataatggc
agataactgtctggaaaagaaatacttgcatgttggagtcgactgttctcaatcaatgaa
tattcaatcttcccgtggatttcttcaagtagttgacgtagttcctccgctatgccgtca
agtattactagttttgtcaactatcgatgattccgcacaatacagagttagcaacagcct
taagacccttgtccatcgttcaaagtgtcttctaccagaaatgtggtacgaagctgccat
ttcggcaccaaacgctcaacactatcctctgttgaacacgtcttctt


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
AATGTCTTCTCGGATCTCTGATAAC 698 163 1242 Antisense
TCGACGAATTTTTTTTGGCTCCAGG 608 599 1277 Antisense
GTATGTCACAGTCAAAGCTCCCTAC 119 455 1315 Antisense
TGTGCACACACGTTATCCAATTTTC 154 557 1371 Antisense
TCAATCTTCCCGTGGATTTCTTCAA 220 633 1605 Antisense
CAAGTAGTTGACGTAGTTCCTCCGC 8 179 1627 Antisense
TCCGCTATGCCGTCAAGTATTACTA 55 597 1647 Antisense
GAGTTAGCAACAGCCTTAAGACCCT 187 395 1706 Antisense
TTAAGACCCTTGTCCATCGTTCAAA 288 683 1721 Antisense
GGTACGAAGCTGCCATTTCGGCACC 351 501 1766 Antisense
TATCCTCTGTTGAACACGTCTTCTT 405 657 1804 Antisense
Sequence Source Affymetrix Proprietary Database