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[188155_at] serine protease

Description F31D4.6 /REP_DB=WormBase Gene ID /WP=CE15932 /TR=O45421 /GB=CAB07374.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Trypsin
Cluster 6grp_total > 62      4grp_total > 19      6grp_cgc > 90      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 3.9
(-3)
A 		10.8
(-2.4)
A 		50.9
(1.3)
A 		69.1
(3)
A 		65
B 44.2
(3)
A 		36.1
(1.7)
A 		10.4
(-2.4)
A 		6.7
(-3)
A 		38
C 26
(-0.4)
A 		50.5
(2.8)
A 		6
(-3)
A 		52
(3)
A 		46
avr 24.7
(-2.3) 		32.5
(-0) 		22.4
(-3) 		42.6
(3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 18.4
(-0.6)
A 		25.1
(0.5)
A 		39.9
(3)
A 		27.9
(1)
A 		33.7
(2)
A 		3.7
(-3)
A 		36
B 8.5
(-3)
A 		11.7
(-2)
A 		25.4
(2.5)
A 		27.1
(3)
A 		26
(2.6)
A 		20.7
(0.9)
A 		19
C 15.5
(1.2)
A 		10.9
(-0.5)
A 		20.4
(3)
A 		3.9
(-3)
A 		7.8
(-1.6)
A 		11.9
(-0.1)
A 		17
avr 14.1
(-2.3) 		15.9
(-1.6) 		28.6
(3) 		19.6
(-0.3) 		22.5
(0.8) 		12.1
(-3) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 1.4
(-3)
A 		2.3
(-2.5)
A 		13.3
(3)
A 		10.1
(1.4)
A 		12
B 14.1
(3)
A 		8.9
(0.5)
A 		2.4
(-2.5)
A 		1.4
(-3)
A 		13
C 8.6
(0.1)
A 		14.5
(3)
A 		2.1
(-3)
A 		8.8
(0.2)
A 		12
avr 8
(1.7) 		8.6
(3) 		5.9
(-3) 		6.8
(-1) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 3.4
(-1.4)
A 		8.9
(2.2)
A 		8.7
(2)
A 		7.1
(1)
A 		10.2
(3)
A 		0.9
(-3)
A 		9
B 3
(-3)
A 		3.9
(-2.3)
A 		6.2
(-0.5)
A 		7.2
(0.3)
A 		10.6
(3)
A 		7.7
(0.7)
A 		8
C 5.8
(3)
A 		3.3
(0)
A 		5.2
(2.3)
A 		0.8
(-3)
A 		2.5
(-1)
A 		4
(0.8)
A 		5
avr 4.1
(-3) 		5.4
(-0.9) 		6.7
(1.2) 		5
(-1.5) 		7.8
(3) 		4.2
(-2.8) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188155_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14641
Sequence Type Exemplar sequence
Representative Public ID F31D4.6 NCBI
Target Description F31D4.6 /REP_DB=WormBase Gene ID /WP=CE15932 /TR=O45421 /GB=CAB07374.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Trypsin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075629(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075629 NCBI Caenorhabditis elegans TRYpsin-like Protease family member (try-4) (try-4) mRNA, complete cds. 11/11 None
SNAP00000041985 ENSEMBL cdna:SNAP chromosome:CEL140:V:20857395:20859033:-1 10/11 None
GENEFINDER00000041995 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20857395:20857953:-1 9/11 None
F31D4.6 ENSEMBL cdna:known chromosome:CEL140:V:20857395:20859033:-1 gene:F31D4.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20857394-20859033(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine protease
Gene Symbol try-4
Ensembl F31D4.6
Entrez Gene 185148 Entrez gene
SwissProt O45421 EMBL-EBI
WormBase CE34005 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4263 chymotrypsin activity inferred from electronic annotation QuickGO AmiGO
4295 trypsin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07374 Hypothetical protein F31D4.6 [Caenorhabditis elegans] ref|NP_508030.2| TRYpsin-like Protease family member (try-4) [Caenorhabditis elegans] 1.0E-176
blast CAB07374 Hypothetical protein F31D4.6 [Caenorhabditis elegans] ref|NP_508030.2| TRYpsin-like Protease family member (try-4) [Caenorhabditis elegans] 1.0E-161
blast CAE61632 Hypothetical protein CBG05562 [Caenorhabditis briggsae] 1.0E-109
blast CAE61632 Hypothetical protein CBG05562 [Caenorhabditis briggsae] 1.0E-101
blast CAB07374 Hypothetical protein F31D4.6 [Caenorhabditis elegans] ref|NP_508030.2| TRYpsin-like Protease family member (try-4) [Caenorhabditis elegans] 7.0E-62
blast CAE61632 Hypothetical protein CBG05562 [Caenorhabditis briggsae] 7.0E-54
blast CAD30839 clip-domain serine protease [Anopheles gambiae] gb|EAA44761.1| ENSANGP00000024671 [Anopheles gambiae str. PEST] ref|XP_312743.1| ENSANGP00000024671 [Anopheles gambiae str. PEST] 4.0E-15
Protein Domains
Database ID Description E-Value
Pfam IPR001254 EMBL-EBI Peptidase S1, chymotrypsin 1.5E-17
Pfam IPR001254 EMBL-EBI Peptidase S1, chymotrypsin 1.1E-16
Pfam IPR001254 EMBL-EBI Peptidase S1, chymotrypsin 2.5E-10
Sequence
Target Sequence 
>C. ELEGANS:188155_AT
gttgacggggaattcgcgtcttccaactgtctaaaaggtcatgactgggcaattgttgaa
gtcgagaaacggattcatttcagcgaaaacgtgcgtccaatttgtcttccacgccctaac
atgtactacacaaagtcattggcagttcctggatggggaagaagttacaggaagtttatt
tttctaatttcaagtttaaaaaaaaacaaatccatttacttttcagtttttaatgagtct
ggcccgctgattcatgagatcccaatgcgaattgatagagactgcaagcgtccttggagt
gataggttaccagcggatgcggacgatttcatatgtgctacttcaatgaatgtttcaaat
tattcagcgcccagaacttgccacggagatagtggaggtggtctcgaatatcgagatgac
aattatggacgtgcttttttaattgcaattacgtcatttggaactcgtgggtgtcccagc
aatatgcttgccaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGACGGGGAATTCGCGTCTTCCA 142 435 259 Antisense
TCGCGTCTTCCAACTGTCTAAAAGG 164 593 272 Antisense
GAAAACGTGCGTCCAATTTGTCTTC 236 353 343 Antisense
CACGCCCTAACATGTACTACACAAA 146 199 368 Antisense
TGGCCCGCTGATTCATGAGATCCCA 238 545 498 Antisense
GAGACTGCAAGCGTCCTTGGAGTGA 704 391 536 Antisense
GTTACCAGCGGATGCGGACGATTTC 345 445 564 Antisense
GGACGATTTCATATGTGCTACTTCA 380 523 579 Antisense
AAATTATTCAGCGCCCAGAACTTGC 346 115 615 Antisense
ATTTGGAACTCGTGGGTGTCCCAGC 552 1 714 Antisense
GTGTCCCAGCAATATGCTTGCCAGA 452 487 729 Antisense
Sequence Source Affymetrix Proprietary Database