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[188153_s_at]

Description Y66H1A.6 /REP_DB=WormBase Gene ID /WP=CE22757 /GEN=hum-8 /TR=Q9TYJ9 /GB=AAD12859.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 56      6grp_high1_10_10 > 65      4grp_highest_8_8 > 25      4grp_high_8_8 > 60      6grp_highest_nonorm > 74      6grp_total > 55      4grp_total > 60      6grp_cgc > 46      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 438
1 2 3 4 range
A 559.2
(-0.2)
P 		643.4
(0.9)
P 		793.4
(3)
P 		355.7
(-3)
P 		438
B 769.9
(3)
P 		740.5
(1.6)
P 		769.3
(3)
P 		642
(-3)
P 		128
C 672.8
(1.7)
P 		641.1
(1)
P 		724.5
(3)
P 		480.1
(-3)
P 		244
avr 667.3
(0.9) 		675
(1.1) 		762.4
(3) 		492.6
(-3) 		270

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 555
1 2 3 4 5 6 range
A 727.3
(1.2)
P 		701.1
(0.7)
P 		805.5
(3)
P 		537.6
(-3)
P 		671
(-0)
P 		615.2
(-1.3)
P 		268
B 700.1
(-0.2)
P 		667.5
(-0.7)
P 		882.2
(2.5)
P 		791.5
(1.2)
P 		509.8
(-3)
P 		915.8
(3)
P 		406
C 454.1
(-3)
P 		753
(0.2)
P 		770.9
(0.4)
P 		653.8
(-0.8)
P 		489.7
(-2.6)
P 		1009.3
(3)
P 		555
avr 627.2
(-1.5) 		707.2
(0.1) 		819.5
(2.4) 		661
(-0.8) 		556.8
(-3) 		846.8
(3) 		290
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 200
1 2 3 4 range
A 203.6
(2.9)
P 		139.7
(0.4)
P 		207.4
(3)
P 		52.1
(-3)
P 		155
B 245.5
(3)
P 		182.9
(-0.5)
P 		178.3
(-0.7)
P 		136.8
(-3)
P 		109
C 222.7
(2)
P 		184.3
(0.6)
P 		252.5
(3)
P 		80.9
(-3)
P 		172
avr 223.9
(3) 		169
(0.5) 		212.7
(2.5) 		89.9
(-3) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 210
1 2 3 4 5 6 range
A 134.1
(-3)
P 		249.3
(3)
P 		175.4
(-0.8)
P 		136.3
(-2.9)
P 		202.5
(0.6)
P 		149.8
(-2.2)
P 		115
B 243.6
(-1.4)
P 		222.2
(-2.3)
P 		214.2
(-2.7)
P 		209.7
(-2.9)
P 		207.1
(-3)
P 		341.5
(3)
P 		134
C 168.3
(-1.9)
P 		229.8
(-0.1)
P 		196.2
(-1.1)
P 		131.5
(-3)
P 		158.5
(-2.2)
P 		337.9
(3)
P 		206
avr 182
(-1.8) 		233.8
(0.8) 		195.3
(-1.2) 		159.2
(-3) 		189.4
(-1.5) 		276.4
(3) 		117
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188153_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10932
Sequence Type Exemplar sequence
Representative Public ID Y66H1A.6 NCBI
Target Description Y66H1A.6 /REP_DB=WormBase Gene ID /WP=CE22757 /GEN=hum-8 /TR=Q9TYJ9 /GB=AAD12859.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028377(11), NM_001028378(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028377 NCBI Caenorhabditis elegans Heavy chain, Unconventional Myosin family member (hum-8) (hum-8) mRNA, complete cds. 11/11 None
NM_001028378 NCBI Caenorhabditis elegans Heavy chain, Unconventional Myosin family member (hum-8) (hum-8) mRNA, complete cds. 11/11 None
SNAP00000007351 ENSEMBL cdna:SNAP chromosome:CEL140:IV:429277:442528:-1 11/11 None
Y66H1A.6a ENSEMBL cdna:known chromosome:CEL140:IV:429213:439734:-1 gene:Y66H1A.6 11/11 A 
Y66H1A.6b ENSEMBL cdna:known chromosome:CEL140:IV:429233:439734:-1 gene:Y66H1A.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000007358 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:429276-439734(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y66H1A.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD12859 Heavy chain, unconventional myosin protein 8, isoform a [Caenorhabditis elegans] ref|NP_001023548.1| Heavy chain, Unconventional Myosin family member (hum-8) [Caenorhabditis elegans] 0.0
blast AAT68888 Heavy chain, unconventional myosin protein 8, isoform b [Caenorhabditis elegans] ref|NP_001023549.1| Heavy chain, Unconventional Myosin family member (hum-8) [Caenorhabditis elegans] 0.0
blast CAE67943 Hypothetical protein CBG13543 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004009 EMBL-EBI Myosin N-terminal SH3-like domain 2.3E-5
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 2.7E-52
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Pfam IPR004009 EMBL-EBI Myosin N-terminal SH3-like domain 2.3E-5
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188153_S_AT
ctccgtcagcgccacaaatacagcgatattttaagtgcgagttcaaggaaaacaatcaga
aagatcaagttagtatgtggttcgtacatttttctggtcagcagattcaacgtcagctga
catttacttcttcaagacctccacaaactttgattgcaggacgagacgatgctcaaatgt
gtactctggcgctacaggagacgcttcttgtcggtaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCCGTCAGCGCCACAAATACAGCG 285 235 3392 Antisense
AGCGATATTTTAAGTGCGAGTTCAA 546 79 3413 Antisense
GGTTCGTACATTTTTCTGGTCAGCA 480 507 3470 Antisense
CTGGTCAGCAGATTCAACGTCAGCT 319 241 3485 Antisense
ACGTCAGCTGACATTTACTTCTTCA 149 95 3501 Antisense
ATTTACTTCTTCAAGACCTCCACAA 151 15 3513 Antisense
GACCTCCACAAACTTTGATTGCAGG 313 383 3527 Antisense
GATTGCAGGACGAGACGATGCTCAA 637 433 3543 Antisense
GATGCTCAAATGTGTACTCTGGCGC 269 413 3559 Antisense
GTACTCTGGCGCTACAGGAGACGCT 639 459 3572 Antisense
ACAGGAGACGCTTCTTGTCGGTAAA 481 107 3585 Antisense
Sequence Source Affymetrix Proprietary Database