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[188150_s_at]

Description K07D4.3 /REP_DB=WormBase Gene ID /WP=CE19527 /GEN=rpn-11 /TR=O76577 /GB=AAC26287.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 93      4grp_highest_8_8 > 12      4grp_high_8_8 > 38      6grp_highest_nonorm > 65      6grp_total > 95      4grp_total > 40      6grp_cgc > 77      6grp_high1_sub > 04      6grp_cgc_sub > 04_05_06      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 813
1 2 3 4 range
A 948.3
(-3)
P 		1709.6
(2.7)
P 		1745.4
(3)
P 		1557.5
(1.6)
P 		797
B 932.1
(-3)
P 		1383
(0.5)
P 		1712.3
(3)
P 		1479.9
(1.2)
P 		780
C 959.1
(-3)
P 		1608.2
(3)
P 		1515.2
(2.1)
P 		1367.8
(0.8)
P 		649
avr 946.5
(-3) 		1566.9
(2.2) 		1657.6
(3) 		1468.4
(1.4) 		711

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 596
1 2 3 4 5 6 range
A 1254.5
(-0.1)
P 		1091.3
(-2.6)
P 		1067.6
(-3)
P 		1332.2
(1)
P 		1265.4
(0)
P 		1460.9
(3)
P 		393
B 958.3
(-2.1)
P 		908.2
(-3)
P 		1254.5
(3)
P 		1165.6
(1.5)
P 		1245.6
(2.8)
P 		1178.2
(1.7)
P 		346
C 1292.5
(2.4)
P 		865.3
(-3)
P 		1259.4
(2)
P 		1164.4
(0.8)
P 		1337.3
(3)
P 		1119.1
(0.2)
P 		472
avr 1168.4
(0.9) 		954.9
(-3) 		1193.8
(1.4) 		1220.7
(1.9) 		1282.8
(3) 		1252.7
(2.4) 		328
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 300
1 2 3 4 range
A 345.3
(0.1)
P 		371.1
(0.8)
P 		456.3
(3)
P 		228
(-3)
P 		228
B 297.2
(-3)
P 		341.6
(-0.3)
P 		396.8
(3)
P 		315.4
(-1.9)
P 		100
C 317.5
(-1.2)
P 		462.4
(1.7)
P 		528.1
(3)
P 		230.6
(-3)
P 		298
avr 320
(-1.2) 		391.7
(1) 		460.4
(3) 		258
(-3) 		202

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 275
1 2 3 4 5 6 range
A 231.3
(-3)
P 		388.1
(3)
P 		232.5
(-3)
P 		337.9
(1.1)
P 		381.8
(2.8)
P 		355.7
(1.8)
P 		157
B 333.4
(-2.1)
P 		302.3
(-3)
P 		304.6
(-2.9)
P 		308.7
(-2.8)
P 		506.1
(3)
P 		439.3
(1)
P 		204
C 479.1
(3)
P 		264
(-2.3)
P 		320.5
(-0.9)
P 		234.2
(-3)
P 		432.7
(1.9)
P 		374.6
(0.4)
P 		245
avr 347.9
(-0.6) 		318.1
(-1.7) 		285.9
(-3) 		293.6
(-2.7) 		440.2
(3) 		389.9
(1) 		154
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188150_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4787
Sequence Type Exemplar sequence
Representative Public ID K07D4.3 NCBI
Target Description K07D4.3 /REP_DB=WormBase Gene ID /WP=CE19527 /GEN=rpn-11 /TR=O76577 /GB=AAC26287.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062311(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062311 NCBI Caenorhabditis elegans proteasome Regulatory Particle, Non-ATPase-like family member (rpn-11) (rpn-11) mRNA, complete cds. 11/11 None
SNAP00000021601 ENSEMBL cdna:SNAP chromosome:CEL140:II:4039100:4044318:-1 11/11 None
GENEFINDER00000021608 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4043211:4044318:-1 11/11 None
K07D4.3.2 ENSEMBL cdna:known chromosome:CEL140:II:4043055:4044331:-1 gene:K07D4.3 11/11 None
K07D4.3.1 ENSEMBL cdna:known chromosome:CEL140:II:4043055:4044324:-1 gene:K07D4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4043209-4044317(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rpn-11
Ensembl K07D4.3
Entrez Gene 173744 Entrez gene
SwissProt O76577 EMBL-EBI
WormBase CE19527 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:249796_AT 26S proteasome regulatory subunit, putative at
CANINE:1586807_AT similar to proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 cfa
CANINE_2:CFA.10188.1.S1_AT similar to proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 cfa
CANINE_2:CFAAFFX.15655.1.S1_S_AT similar to proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 cfa
DROSOPHILA_2:1628531_AT dm
DROSGENOME1:144247_AT dm
CHICKEN:GGA.5295.1.S1_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 gga
CHICKEN:GGAAFFX.12702.1.S1_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 gga
HU35KSUBC:RC_T86751_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 hs
HU35KSUBC:RC_AA621752_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 hs
HUGENEFL:U86782_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 hs
U133_X3P:HS.178761.0.S1_3P_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 hs
HG-U133A_2:212296_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 hs
HG-FOCUS:212296_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 hs
HG-U133_PLUS_2:212296_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 hs
HG-U133A:212296_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 hs
HG-U95AV2:33247_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 hs
HG-U95B:43809_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 hs
HG-U95D:87461_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 hs
MOUSE430_2:1421751_A_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 mm
MOUSE430A_2:1421751_A_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 mm
MOE430A:1421751_A_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 mm
MU11KSUBB:Y13071_S_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 mm
MG-U74AV2:97274_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 mm
MOUSE430_2:1459734_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14, mRNA (cDNA clone MGC:5840 IMAGE:3599671) mm
MOE430B:1459734_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14, mRNA (cDNA clone MGC:5840 IMAGE:3599671) mm
MOUSE430_2:1446521_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14, mRNA (cDNA clone MGC:5840 IMAGE:3599671) mm
MOE430B:1446521_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14, mRNA (cDNA clone MGC:5840 IMAGE:3599671) mm
MG-U74BV2:105181_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14, mRNA (cDNA clone MGC:5840 IMAGE:3599671) mm
MOUSE430_2:1457765_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14, mRNA (cDNA clone MGC:5840 IMAGE:3599671) mm
MOE430B:1457765_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14, mRNA (cDNA clone MGC:5840 IMAGE:3599671) mm
MU19KSUBB:TC33835_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14, mRNA (cDNA clone MGC:5840 IMAGE:3599671) mm
YEAST_2:1770323_AT Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates Sc
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5838 proteasome regulatory particle (sensu Eukaryota) inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5515 protein binding inferred from physical interaction QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC26287 Proteasome regulatory particle, non-atpase-like protein 11 [Caenorhabditis elegans] ref|NP_494712.1| proteasome Regulatory Particle, Non-ATPase-like family member (rpn-11) [Caenorhabditis elegans] sp|O76577|PSDE_CAEEL 26S proteasome non-ATPase regulatory subunit 14 (26S proteasome regulatory subunit rpn11) 1.0E-166
blast CAE56296 Hypothetical protein CBG23950 [Caenorhabditis briggsae] 1.0E-162
blast AAC26287 Proteasome regulatory particle, non-atpase-like protein 11 [Caenorhabditis elegans] ref|NP_494712.1| proteasome Regulatory Particle, Non-ATPase-like family member (rpn-11) [Caenorhabditis elegans] sp|O76577|PSDE_CAEEL 26S proteasome non-ATPase regulatory subunit 14 (26S proteasome regulatory subunit rpn11) 1.0E-161
blast CAE56296 Hypothetical protein CBG23950 [Caenorhabditis briggsae] 1.0E-157
Protein Domains
Database ID Description E-Value
Pfam IPR000555 EMBL-EBI Peptidase M67, Mov34 6.0E-49
Sequence
Target Sequence 
>C. ELEGANS:188150_S_AT
gtcgtcggatggtaccattcccatccaggattcggatgttggctctccggcgtcgacatc
aacactcagcagtcgtttgaagcgctttccgacagagctgtcgccgttgtcgtcgacccg
attcaatctgtgaagggaaaggttgtgatcgacgcgttccgtacgatcaacccacaatcg
atggctttaaatcaagaaccacgtcaaactaccagtaatttgggtcatttgcagaagccg
agcattcaggccctaatccacggactcaatcgtcactactactccatcccaatcgcctac
agaacccacgaccttgaacagaagatgctcttgaatcttaacaagctctcatggatggac
gcagtcagcgtcgaaaactactcaaaatgcggtgaacagaacaaggagcacctcaaggcg
atgctcaagctcgccaagaactacaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCGTCGGATGGTACCATTCCCATC 298 473 340 Antisense
TCCAGGATTCGGATGTTGGCTCTCC 86 591 363 Antisense
GACATCAACACTCAGCAGTCGTTTG 368 369 394 Antisense
TGAAGCGCTTTCCGACAGAGCTGTC 99 575 417 Antisense
AAAGGTTGTGATCGACGCGTTCCGT 247 121 477 Antisense
GATCAACCCACAATCGATGGCTTTA 50 423 504 Antisense
AGAACCACGTCAAACTACCAGTAAT 606 73 534 Antisense
TGCAGAAGCCGAGCATTCAGGCCCT 175 579 569 Antisense
ATCCACGGACTCAATCGTCACTACT 344 39 595 Antisense
CACCTCAAGGCGATGCTCAAGCTCG 661 201 748 Antisense
GCTCAAGCTCGCCAAGAACTACAAG 535 301 762 Antisense
Sequence Source Affymetrix Proprietary Database