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[188144_s_at]

Description C30G12.7 /REP_DB=WormBase Gene ID /WP=CE01838 /GEN=puf-8 /TR=Q09487 /GB=AAC46676.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 08      6grp_cgc > 68      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 342
1 2 3 4 range
A 104.6
(-3)
A
116.3
(-2.8)
A
215.7
(-0.8)
P
403.6
(3)
P
299
B 104.3
(-3)
A
120.6
(-2.5)
A
159.4
(-1.5)
A
317.8
(3)
P
214
C 124.8
(-3)
A
150.3
(-2.5)
A
214.8
(-1.3)
P
446.3
(3)
P
322
avr 111.2
(-3)
129.1
(-2.6)
196.6
(-1.2)
389.2
(3)
278

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1113
1 2 3 4 5 6 range
A 98.4
(-3)
A
134.3
(-2.8)
A
215.6
(-2.4)
P
417.3
(-1.3)
P
872.2
(1.2)
P
1195.5
(3)
P
1097
B 89.5
(-3)
A
105.5
(-2.9)
A
198.5
(-2.4)
P
379
(-1.3)
P
741.3
(0.8)
P
1126.5
(3)
P
1037
C 82.7
(-3)
A
97.2
(-2.9)
A
209.3
(-2.3)
P
403.6
(-1.2)
P
733.7
(0.6)
P
1180.3
(3)
P
1098
avr 90.2
(-3)
112.3
(-2.9)
207.8
(-2.3)
400
(-1.3)
782.4
(0.9)
1167.4
(3)
1077
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 38.1
(-0.7)
A
25.3
(-3)
A
56.4
(2.5)
P
59.1
(3)
P
34
B 33.3
(-2.4)
A
29.8
(-3)
A
36.9
(-1.9)
A
67.7
(3)
P
38
C 41.3
(-3)
A
43.2
(-2.7)
A
74.9
(2.9)
P
75.3
(3)
P
34
avr 37.6
(-2.2)
32.8
(-3)
56.1
(1)
67.4
(3)
35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 402
1 2 3 4 5 6 range
A 18.1
(-3)
A
47.8
(-2.3)
A
47
(-2.4)
P
105.8
(-1.1)
P
263.2
(2.4)
P
291.1
(3)
P
273
B 31.1
(-3)
A
35.1
(-2.9)
A
48.2
(-2.7)
P
100.4
(-1.9)
P
301.2
(1.2)
P
420
(3)
P
389
C 30.7
(-3)
A
29.6
(-3)
A
53.3
(-2.6)
P
81.2
(-2.2)
P
237.4
(0.4)
P
395.1
(3)
P
366
avr 26.6
(-3)
37.5
(-2.8)
49.5
(-2.6)
95.8
(-1.8)
267.3
(1.2)
368.7
(3)
342
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188144_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5425
Sequence Type Exemplar sequence
Representative Public ID C30G12.7 NCBI
Target Description C30G12.7 /REP_DB=WormBase Gene ID /WP=CE01838 /GEN=puf-8 /TR=Q09487 /GB=AAC46676.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063122(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_063122 NCBI Caenorhabditis elegans PUF (Pumilio/FBF) domain-containing family member (puf-8) (puf-8) mRNA, complete cds. 11/11 None
SNAP00000038321 ENSEMBL cdna:SNAP chromosome:CEL140:II:7296235:7298160:-1 11/11 None
GENEFINDER00000038324 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7296235:7298160:-1 11/11 None
C30G12.7 ENSEMBL cdna:known chromosome:CEL140:II:7296084:7298178:-1 gene:C30G12.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7296230-7297551(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol puf-8
Ensembl C30G12.7
Entrez Gene 174197 Entrez gene
SwissProt Q09487 EMBL-EBI
WormBase CE37486 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
3723 RNA binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC46676 Puf (pumilio/fbf) domain-containing protein 8 [Caenorhabditis elegans] ref|NP_495523.3| PUF (Pumilio/FBF) domain-containing family member (puf-8) [Caenorhabditis elegans] 0.0
blast CAE67588 Hypothetical protein CBG13130 [Caenorhabditis briggsae] 0.0
blast AAA81077 Puf (pumilio/fbf) domain-containing protein 9 [Caenorhabditis elegans] ref|NP_508980.2| PUF (Pumilio/FBF) domain-containing family member (puf-9) [Caenorhabditis elegans] 6.0E-89
Protein Domains
Database ID Description E-Value
Pfam IPR001313 EMBL-EBI Pumilio/Puf RNA-binding 1.7E-5
Pfam IPR001313 EMBL-EBI Pumilio/Puf RNA-binding 2.0E-8
Pfam IPR001313 EMBL-EBI Pumilio/Puf RNA-binding 3.5E-12
Pfam IPR001313 EMBL-EBI Pumilio/Puf RNA-binding 1.4E-10
Pfam IPR001313 EMBL-EBI Pumilio/Puf RNA-binding 3.5E-9
Sequence
Target Sequence
>C. ELEGANS:188144_S_AT
gtccacagtacgtccaattcattgtcgatacgcttctagaatccagcaacacaatttacg
agatgtctgtggatccctacggttgtcgtgttgtccaacgttgtctcgaacactgcagtc
cttcgcagacgaagcctgttatcggccaaattcataaacgtttcgacgaaattgcaaata
atcagtacggaaactatgtggttcaacacgtcattgaacatggaagtgaagaggatagaa
tggttattgttactcgtgtctccaacaatctattcgagtttgccacccacaagtactcgt
cgaacgttatcgaaaaatgtttggaacaaggcgcagtttatcacaagtccatgatcgtag
gagctgcgtgtcaccatcaggaaggatctgtaccgattgttgtccaaatgatgaaagatc
agtacgccaactacgttgttcagaaaatgttcgatcaggttacatcagaacagcgccgtg
aactcatcctaactgttcgtccacacattcccgttttgagaca


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GTCCACAGTACGTCCAATTCATTGT 380 477 572 Antisense
TACGAGATGTCTGTGGATCCCTACG 103 649 628 Antisense
GGTTGTCGTGTTGTCCAACGTTGTC 137 507 652 Antisense
TCTCGAACACTGCAGTCCTTCGCAG 534 611 675 Antisense
GCAGACGAAGCCTGTTATCGGCCAA 332 311 696 Antisense
TACTCGTGTCTCCAACAATCTATTC 678 645 822 Antisense
AGGCGCAGTTTATCACAAGTCCATG 517 53 900 Antisense
ATGATCGTAGGAGCTGCGTGTCACC 438 45 922 Antisense
ATCAGAACAGCGCCGTGAACTCATC 197 29 1035 Antisense
TGAACTCATCCTAACTGTTCGTCCA 679 573 1050 Antisense
GTCCACACATTCCCGTTTTGAGACA 505 477 1070 Antisense
Sequence Source Affymetrix Proprietary Database