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[188143_at]

Description F56B3.4 /REP_DB=WormBase Gene ID /WP=CE27171 /GEN=skr-18 /TR=O45107 /GB=AAK29834.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 82      6grp_high1_10_10 > 39      4grp_highest_8_8 > 11      4grp_high_8_8 > 47      6grp_highest_nonorm > 81      6grp_total > 39      4grp_total > 48      6grp_highest_sub > Cu      6grp_cgc > 08      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 297
1 2 3 4 range
A 279.5
(-2.9)
P 		277.2
(-3)
P 		430.5
(3)
P 		325.1
(-1.1)
P 		153
B 133.1
(-3)
P 		304.7
(3)
P 		300.2
(2.8)
P 		237.4
(0.6)
P 		172
C 252.9
(-0.6)
P 		292.5
(3)
P 		252.9
(-0.6)
P 		226.2
(-3)
P 		66
avr 221.8
(-3) 		291.5
(0.9) 		327.9
(3) 		262.9
(-0.7) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 166
1 2 3 4 5 6 range
A 316.6
(3)
P 		281.8
(1.5)
P 		221.2
(-1)
P 		173.8
(-3)
P 		271.6
(1.1)
P 		243.3
(-0.1)
P 		143
B 292.5
(-1.8)
P 		292.4
(-1.9)
P 		291.3
(-2)
P 		284.3
(-3)
P 		309.3
(0.5)
P 		326.7
(3)
P 		42
C 339.9
(3)
P 		227.5
(-2.6)
P 		220.5
(-3)
P 		268.1
(-0.6)
P 		224.2
(-2.8)
P 		299.1
(0.9)
P 		119
avr 316.3
(3) 		267.2
(-1) 		244.3
(-2.8) 		242.1
(-3) 		268.4
(-0.9) 		289.7
(0.8) 		74
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 101.8
(2)
P 		60.2
(-1.8)
P 		112.6
(3)
P 		47.6
(-3)
P 		65
B 42.5
(-3)
P 		75.2
(3)
P 		69.6
(2)
P 		50.6
(-1.5)
P 		33
C 83.7
(2.5)
P 		84.1
(2.5)
P 		88.1
(3)
P 		38.1
(-3)
P 		50
avr 76
(1.1) 		73.2
(0.7) 		90.1
(3) 		45.4
(-3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 58.4
(-1.5)
P 		100.2
(3)
P 		48.2
(-2.6)
P 		44.1
(-3)
P 		82
(1.1)
P 		59.2
(-1.4)
P 		56
B 101.8
(0.4)
P 		97.3
(-0.1)
P 		70.7
(-3)
P 		75.3
(-2.5)
P 		125.7
(3)
P 		121.8
(2.6)
P 		55
C 126
(3)
P 		69.4
(-1.7)
P 		56.1
(-2.8)
P 		53.9
(-3)
P 		72.6
(-1.4)
P 		100.1
(0.8)
P 		72
avr 95.4
(3) 		89
(2) 		58.3
(-2.9) 		57.8
(-3) 		93.4
(2.7) 		93.7
(2.7) 		38
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188143_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10637
Sequence Type Exemplar sequence
Representative Public ID F56B3.4 NCBI
Target Description F56B3.4 /REP_DB=WormBase Gene ID /WP=CE27171 /GEN=skr-18 /TR=O45107 /GB=AAK29834.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028060(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028060 NCBI Caenorhabditis elegans SKp1 Related (ubiquitin ligase complex component) family member (skr-18) (skr-18) mRNA, complete cds. 10/11 None
F56B3.4a ENSEMBL cdna:known chromosome:CEL140:IV:786274:794273:-1 gene:F56B3.4 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001028061 3/11 Cross Hyb Matching Probes None
SNAP00000041567 3/11 Cross Hyb Matching Probes None
GENEFINDER00000041571 5/11 Cross Hyb Matching Probes None
GENEFINDER00000041579 3/11 Cross Hyb Matching Probes None
F56B3.4b 3/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176905 2 1 NM_001028060 188143_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:786329-795412(-) 98.2 100.0
Public Domain and Genome References
Ensembl F56B3.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL32223 Skp1 related (ubiquitin ligase complex component) protein 18, isoform a [Caenorhabditis elegans] ref|NP_001023231.1| SKp1 Related (ubiquitin ligase complex component) family member (skr-18) [Caenorhabditis elegans] 0.0
blast F88632 protein F56B3.4 [imported] - Caenorhabditis elegans 0.0
blast AAU05566 Skp1 related (ubiquitin ligase complex component) protein 18, isoform b [Caenorhabditis elegans] ref|NP_001023232.1| SKp1 Related (ubiquitin ligase complex component) family member (skr-18) [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:188143_AT
accattttgattgccgcttatttgaattctccggttggagatagcatcgaataccgtttc
gccttgtggtcaccaaaacgtaacacaatccgccaaatgacgctttccacgtcatcccag
tcgtgtcgagaccttctcggagcaattaaaacgcagattttagaacgagaaactgtgatt
tatgagcaacattatgtacagtattgctcattgctcggagcatatgttactgcgattagg
gatgatttgaaaaaatggcatagaaatggtttttggaaaaaaaaaatttcggaaaaattt
tttttccaaaaatttcaaaaaaaaaatttattcaaatttttgttcctaaaaataaaaaaa
attgcaaagaaaacttttttttgtggaaaagttttccgaatttttaataaaattcctaaa
aattcaattttgacaaaaatccgaaattccccccaaaaaaatttaaattttcagatcacc
cgtgagcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCATTTTGATTGCCGCTTATTTGA 307 85 2098 Antisense
TGATTGCCGCTTATTTGAATTCTCC 12 565 2105 Antisense
GCTTATTTGAATTCTCCGGTTGGAG 473 303 2113 Antisense
CTCCGGTTGGAGATAGCATCGAATA 597 231 2126 Antisense
AGCATCGAATACCGTTTCGCCTTGT 214 77 2140 Antisense
GTTTCGCCTTGTGGTCACCAAAACG 210 451 2153 Antisense
AACGTAACACAATCCGCCAAATGAC 549 143 2174 Antisense
AACACAATCCGCCAAATGACGCTTT 668 133 2179 Antisense
CAAATGACGCTTTCCACGTCATCCC 229 193 2191 Antisense
TCGTGTCGAGACCTTCTCGGAGCAA 308 605 2218 Antisense
TAAATTTTCAGATCACCCGTGAGCG 509 639 2561 Antisense
Sequence Source Affymetrix Proprietary Database