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[188142_s_at]

Description C09G12.8A /REP_DB=WormBase Gene ID /WP=CE16832 /GEN=rac-1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster 6grp_total > 18      4grp_total > 02      6grp_cgc > 11      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 134
1 2 3 4 range
A 8.3
(-3)
A 		12.2
(-2.8)
A 		10.1
(-2.9)
A 		138.3
(3)
A 		130
B 11.2
(-2.9)
A 		10.6
(-3)
A 		13.6
(-2.7)
A 		81.5
(3)
P 		71
C 6.2
(-2.9)
A 		4.7
(-3)
A 		8.2
(-2.8)
A 		90.3
(3)
M 		86
avr 8.6
(-3) 		9.2
(-3) 		10.6
(-2.9) 		103.4
(3) 		95

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 53.6
(3)
A 		11.2
(-2)
A 		8
(-2.4)
A 		15.5
(-1.5)
A 		2.7
(-3)
A 		2.5
(-3)
A 		51
B 23
(3)
A 		8.7
(-1.1)
A 		11.7
(-0.3)
A 		5.3
(-2.1)
A 		2.2
(-3)
A 		12.4
(-0.1)
A 		21
C 37.6
(3)
A 		8.3
(-2.2)
A 		4.1
(-2.9)
A 		3.6
(-3)
A 		6.8
(-2.4)
A 		11
(-1.7)
A 		34
avr 38.1
(3) 		9.4
(-2) 		7.9
(-2.3) 		8.1
(-2.3) 		3.9
(-3) 		8.6
(-2.2) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 3
(-2.9)
A 		2.6
(-3)
A 		2.6
(-3)
A 		20.3
(3)
A 		18
B 3.6
(-2.6)
A 		2.6
(-3)
A 		3.1
(-2.8)
A 		17.4
(3)
P 		15
C 2.1
(-2.7)
A 		1.4
(-3)
A 		2.8
(-2.4)
A 		15.2
(3)
M 		14
avr 2.9
(-2.7) 		2.2
(-3) 		2.8
(-2.8) 		17.6
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 9.9
(3)
A 		4
(-0.8)
A 		1.7
(-2.3)
A 		3.9
(-0.9)
A 		0.8
(-2.9)
A 		0.6
(-3)
A 		9
B 8
(3)
A 		2.9
(-1.3)
A 		2.9
(-1.3)
A 		1.4
(-2.6)
A 		0.9
(-3)
A 		4.6
(0.1)
A 		7
C 13.9
(3)
A 		2.5
(-2.2)
A 		1
(-2.9)
A 		0.7
(-3)
A 		2.2
(-2.3)
A 		3.7
(-1.6)
A 		13
avr 10.6
(3) 		3.1
(-1.8) 		1.9
(-2.6) 		2
(-2.5) 		1.3
(-3) 		3
(-1.9) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188142_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10393
Sequence Type Exemplar sequence
Representative Public ID C09G12.8A NCBI
Target Description C09G12.8A /REP_DB=WormBase Gene ID /WP=CE16832 /GEN=rac-1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067962(11), NM_067961(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067962 NCBI Caenorhabditis elegans CEll Death abnormality family member (ced-10) (ced-10) mRNA, complete cds. 11/11 None
NM_067961 NCBI Caenorhabditis elegans CEll Death abnormality family member (ced-10) (ced-10) mRNA, complete cds. 11/11 A 
GENEFINDER00000001989 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3507048:3509490:-1 11/11 None
C09G12.8b ENSEMBL cdna:known chromosome:CEL140:IV:3506397:3509524:-1 gene:C09G12.8 11/11 A 
C09G12.8a ENSEMBL cdna:known chromosome:CEL140:IV:3506397:3509504:-1 gene:C09G12.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3507381-3509496(-) 100.0 100.0
Public Domain and Genome References
Ensembl C09G12.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC25821 Cell death abnormality protein 10, isoform b [Caenorhabditis elegans] ref|NP_500363.1| CEll Death abnormality family member (ced-10) [Caenorhabditis elegans] gb|AAF33846.1| cell-corpse engulfment protein CED-10 [Caenorhabditis elegans] sp|Q03206|RAC1_CAEEL RAS-related protein rac-1 (Cell-corpse engulfment protein ced-10) (CErac1) 1.0E-108
blast CAA48506 small ras-related protein [Caenorhabditis elegans] gb|AAA28141.1| rac1 protein gb|AAA28140.1| rac1 protein 1.0E-107
blast AAC25821 Cell death abnormality protein 10, isoform b [Caenorhabditis elegans] ref|NP_500363.1| CEll Death abnormality family member (ced-10) [Caenorhabditis elegans] gb|AAF33846.1| cell-corpse engulfment protein CED-10 [Caenorhabditis elegans] sp|Q03206|RAC1_CAEEL RAS-related protein rac-1 (Cell-corpse engulfment protein ced-10) (CErac1) 1.0E-105
blast CAA48506 small ras-related protein [Caenorhabditis elegans] gb|AAA28141.1| rac1 protein gb|AAA28140.1| rac1 protein 1.0E-105
blast CAB05247 Hypothetical protein K03D3.10a [Caenorhabditis elegans] ref|NP_502936.1| RAC related family member (rac-2) [Caenorhabditis elegans] sp|Q94124|RAC2_CAEEL RAS-related protein rac-2 gb|AAB40386.1| CeRac2 9.0E-97
blast CAB05247 Hypothetical protein K03D3.10a [Caenorhabditis elegans] ref|NP_502936.1| RAC related family member (rac-2) [Caenorhabditis elegans] sp|Q94124|RAC2_CAEEL RAS-related protein rac-2 gb|AAB40386.1| CeRac2 4.0E-94
blast AAC25820 Cell death abnormality protein 10, isoform a [Caenorhabditis elegans] ref|NP_500362.3| CEll Death abnormality family member (ced-10) [Caenorhabditis elegans] 2.0E-69
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 8.8E-90
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 6.4E-39
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 2.0E-11
Sequence
Target Sequence 
>C. ELEGANS:188142_S_AT
taaaacgtgtctcctgatatcctacaccacaaacgcatttcccggagaatatattccgac
ggtattcgacaactactcagcaaatgtgatggtcgacggtcggccgataaatctcgggct
ctgggatacagctggacaggaagattacgatcggctccgcgaacgccggctccaaccagt
gagccaaacccagggctacgtgatggcaaaggaaatcaaggctgtcaagtatctggagtg
ctcggcgctcacgcaacgtggtctgaaacaagttttcgatgaggcgatccgagccgtgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAAACGTGTCTCCTGATATCCTAC 42 639 57 Antisense
GACGGTATTCGACAACTACTCAGCA 191 377 114 Antisense
TGTGATGGTCGACGGTCGGCCGATA 362 555 141 Antisense
GGTCGGCCGATAAATCTCGGGCTCT 283 501 154 Antisense
TCTCGGGCTCTGGGATACAGCTGGA 669 611 168 Antisense
CCAGTGAGCCAAACCCAGGGCTACG 421 265 232 Antisense
GCCAAACCCAGGGCTACGTGATGGC 442 277 239 Antisense
GAAATCAAGGCTGTCAAGTATCTGG 289 363 268 Antisense
TCAAGTATCTGGAGTGCTCGGCGCT 235 633 281 Antisense
TCACGCAACGTGGTCTGAAACAAGT 575 625 305 Antisense
TTCGATGAGGCGATCCGAGCCGTGC 388 695 331 Antisense
Sequence Source Affymetrix Proprietary Database