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[188136_at]

Description C24B5.2 /REP_DB=WormBase Gene ID /WP=CE20522 /TR=Q18128 /GB=AAD31547.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=ATPase
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 88      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 93      6grp_total > 97      4grp_total > 39      6grp_highest_sub > In      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 335.5
(3)
P 		304.3
(-0.2)
P 		277.4
(-3)
P 		321.2
(1.5)
P 		58
B 376.3
(3)
P 		347.4
(-3)
P 		349.9
(-2.5)
P 		366.6
(1)
P 		29
C 432.2
(3)
P 		263.4
(-3)
P 		393.8
(1.6)
P 		314.5
(-1.2)
P 		169
avr 381.3
(3) 		305
(-3) 		340.4
(-0.2) 		334.1
(-0.7) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 733
1 2 3 4 5 6 range
A 266.5
(-3)
P 		310.1
(-2.4)
P 		340.1
(-2)
P 		378.9
(-1.5)
P 		575.9
(1.3)
P 		702.4
(3)
P 		436
B 302.5
(-3)
P 		321.1
(-2.8)
P 		419.1
(-1.4)
P 		414.8
(-1.5)
P 		618
(1.2)
P 		752.7
(3)
P 		450
C 306.5
(-2.4)
P 		236.4
(-3)
P 		322.5
(-2.3)
P 		350.9
(-2.1)
P 		535.6
(-0.6)
P 		969.6
(3)
P 		733
avr 291.8
(-3) 		289.2
(-3) 		360.6
(-2.2) 		381.5
(-1.9) 		576.5
(0.3) 		808.2
(3) 		519
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 122.2
(3)
P 		66.1
(-1.5)
P 		72.5
(-1)
P 		47
(-3)
P 		75
B 120
(3)
P 		85.8
(-1.9)
P 		81.1
(-2.6)
P 		78.1
(-3)
P 		42
C 143.1
(3)
P 		75.7
(-1.5)
P 		137.2
(2.6)
P 		53
(-3)
P 		90
avr 128.4
(3) 		75.9
(-1.6) 		96.9
(0.3) 		59.4
(-3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 276
1 2 3 4 5 6 range
A 49.1
(-3)
P 		110.3
(-0.1)
P 		74.1
(-1.8)
P 		96.1
(-0.7)
P 		173.8
(3)
P 		171
(2.9)
P 		125
B 105.2
(-2.9)
P 		106.9
(-2.8)
P 		101.8
(-3)
P 		109.9
(-2.7)
P 		251.1
(2)
P 		280.7
(3)
P 		179
C 113.6
(-2)
P 		72.1
(-3)
P 		82.1
(-2.7)
P 		70.6
(-3)
P 		173.3
(-0.6)
P 		324.6
(3)
P 		254
avr 89.3
(-2.9) 		96.4
(-2.6) 		86
(-3) 		92.2
(-2.8) 		199.4
(0.9) 		258.8
(3) 		173
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188136_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11955
Sequence Type Exemplar sequence
Representative Public ID C24B5.2 NCBI
Target Description C24B5.2 /REP_DB=WormBase Gene ID /WP=CE20522 /TR=Q18128 /GB=AAD31547.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=ATPase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171501(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171501 NCBI Caenorhabditis elegans C24B5.2a (ATPase) mRNA, complete cds. 11/11 A 
SNAP00000040463 ENSEMBL cdna:SNAP chromosome:CEL140:V:9178388:9180417:1 11/11 None
GENEFINDER00000040469 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9176674:9180417:1 11/11 None
C24B5.2a ENSEMBL cdna:known chromosome:CEL140:V:9178383:9180571:1 gene:C24B5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179300 2 1 NM_171501 174154_at (11), 174155_s_at (11), 188136_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9178388-9180418(+) 100.0 100.0
Public Domain and Genome References
Ensembl C24B5.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM29664 Hypothetical protein C24B5.2a [Caenorhabditis elegans] ref|NP_741586.1| C24B5.2a [Caenorhabditis elegans] 0.0
blast CAE72124 Hypothetical protein CBG19220 [Caenorhabditis briggsae] 0.0
blast AAD31546 Prion-like-(q/n-rich)-domain-bearing protein protein 16 [Caenorhabditis elegans] ref|NP_505378.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-16) [Caenorhabditis elegans] 9.0E-96
blast AAQ74774 spastin [Danio rerio] 5.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR003959 EMBL-EBI AAA ATPase, central region 8.5E-70
Pfam IPR003959 EMBL-EBI AAA ATPase, central region 8.5E-70
Pfam IPR009475 EMBL-EBI Protein of unknown function DUF1096 2.1E-37
Pfam IPR003959 EMBL-EBI AAA ATPase, central region 8.5E-70
Sequence
Target Sequence 
>C. ELEGANS:188136_AT
gcatcttctctaacgtcaaaatgggtcggtgactccgagaaaacaattcgaggtctattt
caaattgctcgaaatgcacaaccatccatcattttcattgatgaaattgactcgatcctt
tgtgagcgatcggaaaaagacgcggaggtatctagaagaatgaaaactgaatttctcgtc
caattcgatggtgcaacgtcatcagcagatgatcgcattcttgtaattggcgccacaaac
aggccacacgagctcgacgatgctgttttgagacgtttcccgaaacgaattatgcttaat
ttgccagatgaagaagcaaggaaggagctgatcacaaagactttgaagaagcacaatatg
atggatggattgataagtagtgatattagatacatagcttccaatacaagcgggttttcg
aactctgatctagttgctttgtgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCATCTTCTCTAACGTCAAAATGGG 407 309 925 Antisense
AATTGCTCGAAATGCACAACCATCC 3 179 987 Antisense
GCACAACCATCCATCATTTTCATTG 598 317 1000 Antisense
ATTGACTCGATCCTTTGTGAGCGAT 217 5 1030 Antisense
AATTTCTCGTCCAATTCGATGGTGC 422 175 1094 Antisense
GATGGTGCAACGTCATCAGCAGATG 678 407 1111 Antisense
TCGCATTCTTGTAATTGGCGCCACA 261 601 1137 Antisense
AATTGGCGCCACAAACAGGCCACAC 208 179 1149 Antisense
CCACACGAGCTCGACGATGCTGTTT 679 273 1168 Antisense
ACAAGCGGGTTTTCGAACTCTGATC 620 111 1330 Antisense
GAACTCTGATCTAGTTGCTTTGTGT 368 349 1344 Antisense
Sequence Source Affymetrix Proprietary Database