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[188131_s_at]

Description F34D6.3 /REP_DB=WormBase Gene ID /WP=CE28297 /GEN=twk-23 /TR=O17185 /GB=AAC71151.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 13      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 90      6grp_total > 14      4grp_total > 14      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 156
1 2 3 4 range
A 193.7
(3)
P 		155.9
(0.1)
P 		115.1
(-3)
P 		185.2
(2.4)
P 		79
B 185.7
(1.6)
P 		116.5
(-3)
P 		163.1
(0.1)
P 		206.9
(3)
P 		90
C 186.3
(-0.3)
P 		119.5
(-2.9)
P 		118.2
(-3)
P 		270.6
(3)
P 		152
avr 188.6
(0.9) 		130.6
(-3) 		132.1
(-2.9) 		220.9
(3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 228.8
(3)
P 		210.6
(1.9)
P 		182.1
(0.3)
P 		163.5
(-0.8)
P 		124.9
(-3)
P 		138.8
(-2.2)
P 		104
B 224.1
(3)
P 		208.4
(2.1)
P 		154.9
(-1.2)
P 		167.3
(-0.4)
P 		124.6
(-3)
P 		126.9
(-2.9)
P 		100
C 194.2
(2.4)
P 		205.5
(3)
P 		130.8
(-0.8)
P 		136.3
(-0.5)
P 		123.2
(-1.2)
P 		87.1
(-3)
P 		118
avr 215.7
(3) 		208.2
(2.5) 		155.9
(-0.7) 		155.7
(-0.7) 		124.2
(-2.6) 		117.6
(-3) 		98
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 70.5
(3)
P 		33.8
(-2.1)
P 		30.1
(-2.6)
P 		27.1
(-3)
P 		43
B 59.2
(3)
P 		28.8
(-3)
P 		37.8
(-1.2)
P 		44.1
(0)
P 		30
C 61.7
(3)
P 		34.4
(-3)
P 		41.2
(-1.5)
P 		45.6
(-0.5)
P 		27
avr 63.8
(3) 		32.3
(-3) 		36.4
(-2.2) 		38.9
(-1.7) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 42.2
(-1.8)
P 		74.9
(3)
P 		39.6
(-2.2)
P 		41.5
(-1.9)
P 		37.7
(-2.4)
P 		33.8
(-3)
P 		41
B 78
(3)
P 		69.4
(1.7)
P 		37.6
(-3)
P 		44.3
(-2)
P 		50.6
(-1.1)
P 		47.3
(-1.6)
P 		40
C 72
(3)
P 		62.7
(1.7)
P 		33.3
(-2.2)
P 		27.4
(-3)
P 		39.9
(-1.3)
P 		29.2
(-2.8)
P 		45
avr 64.1
(2.1) 		69
(3) 		36.8
(-3) 		37.7
(-2.8) 		42.7
(-1.9) 		36.8
(-3) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188131_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5747
Sequence Type Exemplar sequence
Representative Public ID F34D6.3 NCBI
Target Description F34D6.3 /REP_DB=WormBase Gene ID /WP=CE28297 /GEN=twk-23 /TR=O17185 /GB=AAC71151.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061932(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061932 NCBI Caenorhabditis elegans SUPpressor family member (sup-9) (sup-9) mRNA, complete cds. 11/11 None
SNAP00000012249 ENSEMBL cdna:SNAP chromosome:CEL140:II:2684749:2686034:-1 11/11 None
GENEFINDER00000012262 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2684749:2686977:-1 11/11 None
F34D6.3 ENSEMBL cdna:known chromosome:CEL140:II:2684673:2687047:-1 gene:F34D6.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2684747-2686976(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sup-9
Ensembl F34D6.3
Entrez Gene 173613 Entrez gene
SwissProt O17185 EMBL-EBI
WormBase CE28297 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1625302_AT dm
DROSGENOME1:149852_AT dm
HG-U133_PLUS_2:205952_AT potassium channel, subfamily K, member 3 hs
HG-U133A:205952_AT potassium channel, subfamily K, member 3 hs
HG-FOCUS:205952_AT potassium channel, subfamily K, member 3 hs
HG-U133A_2:205952_AT potassium channel, subfamily K, member 3 hs
HU35KSUBA:W40513_AT potassium channel, subfamily K, member 3 hs
U133_X3P:G4504848_3P_X_AT potassium channel, subfamily K, member 3 hs
U133_X3P:205952_3P_AT potassium channel, subfamily K, member 3 hs
HG-U95AV2:41325_AT potassium channel, subfamily K, member 3 hs
HG-U133_PLUS_2:235108_AT Potassium channel, subfamily K, member 3 hs
HG-U133B:235108_AT Potassium channel, subfamily K, member 3 hs
U133_X3P:HS.290791.0.A1_3P_AT Potassium channel, subfamily K, member 3 hs
U133_X3P:HS.24040.1.S1_3P_AT Potassium channel, subfamily K, member 3 hs
HG-U133_PLUS_2:228127_AT Potassium channel, subfamily K, member 3 hs
HG-U133B:228127_AT Potassium channel, subfamily K, member 3 hs
HG-U95E:74268_AT Potassium channel, subfamily K, member 3 hs
HG-U95E:88977_AT Potassium channel, subfamily K, member 3 hs
HG-U95AV2:41326_AT Potassium channel, subfamily K, member 3 hs
HU35KSUBA:R26213_AT Potassium channel, subfamily K, member 3 hs
MOUSE430_2:1426058_A_AT potassium channel, subfamily K, member 3 mm
MOE430A:1426058_A_AT potassium channel, subfamily K, member 3 mm
MOUSE430A_2:1426058_A_AT potassium channel, subfamily K, member 3 mm
MOUSE430A_2:1425341_AT potassium channel, subfamily K, member 3 mm
MOE430A:1425341_AT potassium channel, subfamily K, member 3 mm
MOUSE430_2:1425341_AT potassium channel, subfamily K, member 3 mm
MU11KSUBA:AF006824_AT potassium channel, subfamily K, member 3 mm
MG-U74AV2:102020_AT potassium channel, subfamily K, member 3 mm
MG-U74BV2:106141_AT potassium channel, subfamily K, member 3 mm
MOE430A:1425342_A_AT potassium channel, subfamily K, member 3 mm
MOUSE430A_2:1425342_A_AT potassium channel, subfamily K, member 3 mm
MOUSE430_2:1425342_A_AT potassium channel, subfamily K, member 3 mm
MU19KSUBB:TC26063_S_AT CTBAK mm
RN-U34:RC_AA925138_S_AT potassium channel, subfamily K, member 3 rn
RG-U34A:RC_AA925138_S_AT potassium channel, subfamily K, member 3 rn
RN-U34:AF031384_AT potassium channel, subfamily K, member 3 rn
RG-U34A:AF031384_AT potassium channel, subfamily K, member 3 rn
RAE230A:1398313_A_AT potassium channel, subfamily K, member 3 rn
RAT230_2:1398313_A_AT potassium channel, subfamily K, member 3 rn
RAE230A:1387441_AT potassium channel, subfamily K, member 3 rn
RAT230_2:1387441_AT potassium channel, subfamily K, member 3 rn
RAT230_2:1375807_AT Potassium channel, subfamily K, member 3 rn
RAE230A:1375807_AT Potassium channel, subfamily K, member 3 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6811 ion transport inferred from electronic annotation QuickGO AmiGO
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5216 ion channel activity inferred from electronic annotation QuickGO AmiGO
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC71151 Suppressor protein 9 [Caenorhabditis elegans] ref|NP_494333.1| SUPpressor family member (sup-9) [Caenorhabditis elegans] gb|AAQ84518.1| two-pore K+ channel [Caenorhabditis elegans] sp|O17185|SUP9_CAEEL Two pore potassium channel protein sup-9 (Suppressor of unc-93 protein 9) (n2P38) gb|AAC32863.1| putative potassium channel subunit n2P38 [Caenorhabditis elegans] 0.0
blast CAE72404 Hypothetical protein CBG19563 [Caenorhabditis briggsae] 0.0
blast CAE72404 Hypothetical protein CBG19563 [Caenorhabditis briggsae] 1.0E-178
blast AAC71151 Suppressor protein 9 [Caenorhabditis elegans] ref|NP_494333.1| SUPpressor family member (sup-9) [Caenorhabditis elegans] gb|AAQ84518.1| two-pore K+ channel [Caenorhabditis elegans] sp|O17185|SUP9_CAEEL Two pore potassium channel protein sup-9 (Suppressor of unc-93 protein 9) (n2P38) gb|AAC32863.1| putative potassium channel subunit n2P38 [Caenorhabditis elegans] 1.0E-130
blast CAE72404 Hypothetical protein CBG19563 [Caenorhabditis briggsae] 1.0E-122
blast XP_394509 PREDICTED: similar to GA21932-PA [Apis mellifera] 4.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR005821 EMBL-EBI Ion transport protein 5.7E-11
Pfam IPR005821 EMBL-EBI Ion transport protein 5.7E-11
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494333.1 6 7-29,80-99,106-128,158-180,189-206,221-243
F34D6.3 6 7-29,80-99,106-128,158-180,189-206,221-243
SNAP00000012249 4 7-29,57-79,88-105,120-142
GENEFINDER00000012262 5 7-29,80-99,106-128,158-180,235-257
Sequence
Target Sequence 
>C. ELEGANS:188131_S_AT
tgaatactttcgccgcaaagctgctccgtttcataagacgagccgccgggaaacaaccaa
tagtgacgtcatcagacctgattattttctgtaccgggtggggaggtctgcttatttttg
gaggagcattcatgttttcatcctatgaaaattggacatatttcgacgcggtgtactact
gttttgtgactttgactacaattggattcggtgactacgtggcacttcaaaagcgtggct
ccctgcaaactcaaccagaatacgtattcttctcgctggtcttcattctcttcgggctca
cggtcatttctgcggcgatgaacctcctagtgctccggtttttgactatgaacaccgaag
atgaacggagggatgaacaagaagcgattttggcggctcagggactcgtcagagttggtg
atcctactgctgatgatgattttggcagattaccactgtcggataatgtttcgctcgcct
cctgctcctgctatcagttgccagatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAATACTTTCGCCGCAAAGCTGCT 422 573 428 Antisense
AAAGCTGCTCCGTTTCATAAGACGA 642 123 444 Antisense
GGGGAGGTCTGCTTATTTTTGGAGG 151 497 527 Antisense
TATTTCGACGCGGTGTACTACTGTT 225 661 586 Antisense
GTGGCACTTCAAAAGCGTGGCTCCC 282 489 646 Antisense
AGAATACGTATTCTTCTCGCTGGTC 437 75 684 Antisense
TCACGGTCATTTCTGCGGCGATGAA 260 625 725 Antisense
AAGCGATTTTGGCGGCTCAGGGACT 485 149 809 Antisense
GTCAGAGTTGGTGATCCTACTGCTG 40 465 835 Antisense
ACTGTCGGATAATGTTTCGCTCGCC 418 95 882 Antisense
TGCTCCTGCTATCAGTTGCCAGATG 362 581 910 Antisense
Sequence Source Affymetrix Proprietary Database