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[188129_s_at] myosin

Description F58G4.1 /REP_DB=WormBase Gene ID /WP=CE07306 /TR=Q21000 /GB=AAG24132.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=myosin
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 16      4grp_highest_8_8 > 61      4grp_high_8_8 > 17      6grp_highest_nonorm > 40      6grp_total > 16      4grp_total > 30      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1138
1 2 3 4 range
A 1401.6
(3)
P 		466.9
(-2.2)
P 		314.5
(-3)
P 		1082.5
(1.2)
P 		1087
B 1320.9
(3)
P 		534
(-1.5)
P 		263.8
(-3)
P 		914.2
(0.7)
P 		1057
C 1335.3
(3)
P 		561
(-1.7)
P 		346.3
(-3)
P 		941
(0.6)
P 		989
avr 1352.6
(3) 		520.6
(-1.8) 		308.2
(-3) 		979.2
(0.9) 		1044

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1309
1 2 3 4 5 6 range
A 776.3
(1.1)
P 		1023.4
(3)
P 		241.2
(-3)
P 		309.9
(-2.5)
P 		319.5
(-2.4)
P 		321.1
(-2.4)
P 		782
B 1025.1
(3)
P 		717.4
(0.7)
P 		347.3
(-2.1)
P 		250.4
(-2.8)
P 		226.9
(-3)
P 		333.7
(-2.2)
P 		798
C 1536.3
(3)
P 		754
(-0.7)
P 		587.2
(-1.5)
P 		268.6
(-3)
P 		446
(-2.2)
P 		347.2
(-2.6)
P 		1268
avr 1112.6
(3) 		831.6
(1) 		391.9
(-2.2) 		276.3
(-3) 		330.8
(-2.6) 		334
(-2.6) 		836
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 449
1 2 3 4 range
A 510.3
(3)
P 		101.4
(-2.7)
P 		82.2
(-3)
P 		158.5
(-1.9)
P 		428
B 421.2
(3)
P 		131.9
(-1.8)
P 		61.1
(-3)
P 		194.8
(-0.8)
P 		360
C 442
(3)
P 		161.3
(-2.2)
P 		120.7
(-3)
P 		158.7
(-2.3)
P 		321
avr 457.8
(3) 		131.5
(-2.3) 		88
(-3) 		170.7
(-1.7) 		370

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 517
1 2 3 4 5 6 range
A 143.1
(-1.3)
P 		364
(3)
P 		52.5
(-3)
P 		78.6
(-2.5)
P 		96.4
(-2.2)
P 		78.2
(-2.5)
P 		312
B 356.7
(3)
P 		238.8
(0.6)
P 		84.3
(-2.6)
P 		66.3
(-3)
P 		92.2
(-2.5)
P 		124.5
(-1.8)
P 		290
C 569.4
(3)
P 		230.1
(-0.9)
P 		149.5
(-1.9)
P 		54
(-3)
P 		144.3
(-1.9)
P 		116.2
(-2.3)
P 		515
avr 356.4
(3) 		277.6
(1.4) 		95.4
(-2.4) 		66.3
(-3) 		111
(-2.1) 		106.3
(-2.2) 		290
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188129_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11481
Sequence Type Exemplar sequence
Representative Public ID F58G4.1 NCBI
Target Description F58G4.1 /REP_DB=WormBase Gene ID /WP=CE07306 /TR=Q21000 /GB=AAG24132.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=myosin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072693(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072693 NCBI Caenorhabditis elegans F58G4.1 (myosin) mRNA, complete cds. 11/11 None
SNAP00000010859 ENSEMBL cdna:SNAP chromosome:CEL140:V:8111642:8120432:1 11/11 None
GENEFINDER00000010865 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8111642:8120432:1 11/11 None
F58G4.1 ENSEMBL cdna:known chromosome:CEL140:V:8111642:8120658:1 gene:F58G4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8111642-8120433(+) 100.0 100.0
Public Domain and Genome References
Gene Title myosin
Ensembl F58G4.1
Entrez Gene 179190 Entrez gene
SwissProt Q21000 EMBL-EBI
WormBase CE07306 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.3670.1.S1_X_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal cfa
CANINE_2:CFAAFFX.26800.1.S1_S_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal cfa
CHICKEN:GGA.4217.1.S1_A_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal gga
CHICKEN:GGA.4217.6.S1_X_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal gga
HG-U133_PLUS_2:206717_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal hs
HG-U133A:206717_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal hs
HG-FOCUS:206717_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal hs
HG-U133A_2:206717_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal hs
HUGENEFL:Z38133_S_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal hs
U133_X3P:G4505300_3P_S_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal hs
U133_X3P:G4505300_3P_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal hs
U133_X3P:206717_3P_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal hs
HG-U133A_2:34471_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal hs
HG-FOCUS:34471_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal hs
HG-U133_PLUS_2:34471_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal hs
HG-U133A:34471_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal hs
HG-U95AV2:34471_AT myosin, heavy polypeptide 8, skeletal muscle, perinatal hs
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16459 myosin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3774 motor activity inferred from electronic annotation QuickGO AmiGO
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAG24132 Hypothetical protein F58G4.1 [Caenorhabditis elegans] ref|NP_505094.1| F58G4.1 [Caenorhabditis elegans] 0.0
blast CAE72546 Hypothetical protein CBG19730 [Caenorhabditis briggsae] 0.0
blast CAB05505 Hypothetical protein F11C3.3 [Caenorhabditis elegans] emb|CAB04089.1| Hypothetical protein F11C3.3 [Caenorhabditis elegans] ref|NP_493596.1| UNCoordinated family member (unc-54) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004009 EMBL-EBI Myosin N-terminal SH3-like domain 1.3E-21
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Pfam IPR002928 EMBL-EBI Myosin tail 1.0E-126
Pfam IPR004009 EMBL-EBI Myosin N-terminal SH3-like domain 1.3E-21
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Pfam IPR002928 EMBL-EBI Myosin tail 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188129_S_AT
ggatgcttcgaaacttgctgatgagcttcgttccgagcaagaacacgctagcaatttgaa
ccaatcaaagaaaactcttgaatcacaagttaaagacttgcaaatgagattggatgaagc
tgaagctgctggaattaagggaggaaagagacagttggctaagttagatatgagaattca
tgagttggaaaccgaattggaaggagaaaatagaagacatgctgagacacagaaagtgct
tagaaataaggatagaaaatgcagagagctccagttccaggtcgacgaagacaaaaagtc
ccaagaaagaatgtacgatcttattgaaaagcttcaacagaagatcaagacatacaaaag
acagatcgaagatgcggaatcattggcctctggcaacttggccaaataccgccaacttca
acacgtcgtggaagatgctcaagaaagagctgacgccgctgagaatgctctccaaaagtt
gagactgaagggaaggagtacatcaggagttttcggaccacgaggattggctcactctat
gtcaacgactggagtcaatatgagacgaggtggttctcgaggcgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATGCTTCGAAACTTGCTGATGAG 694 513 5322 Antisense
CTGATGAGCTTCGTTCCGAGCAAGA 201 239 5339 Antisense
GAACACGCTAGCAATTTGAACCAAT 501 351 5362 Antisense
ATGCAGAGAGCTCCAGTTCCAGGTC 58 43 5580 Antisense
GAAGATGCGGAATCATTGGCCTCTG 478 337 5689 Antisense
GGCCTCTGGCAACTTGGCCAAATAC 403 543 5706 Antisense
GGCCAAATACCGCCAACTTCAACAC 225 543 5721 Antisense
CAACACGTCGTGGAAGATGCTCAAG 582 189 5740 Antisense
AAGAGCTGACGCCGCTGAGAATGCT 513 153 5766 Antisense
ACATCAGGAGTTTTCGGACCACGAG 482 101 5821 Antisense
GAGACGAGGTGGTTCTCGAGGCGCA 234 393 5883 Antisense
Sequence Source Affymetrix Proprietary Database