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[188128_at] amylase

Description C50B6.7 /REP_DB=WormBase Gene ID /WP=CE08876 /TR=P91982 /GB=CAB02856.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=amylase
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 52      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 52      6grp_total > 52      4grp_total > 54      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 419
1 2 3 4 range
A 716.1
(3)
P 		577.6
(0.3)
P 		445.3
(-2.3)
P 		408.3
(-3)
P 		308
B 795.4
(3)
P 		529.1
(-0.9)
P 		570.3
(-0.3)
P 		390.7
(-3)
P 		405
C 756.1
(3)
P 		522.6
(-0.7)
P 		517.4
(-0.8)
P 		376.4
(-3)
P 		380
avr 755.9
(3) 		543.1
(-0.5) 		511
(-1) 		391.8
(-3) 		364

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 680
1 2 3 4 5 6 range
A 536.4
(-1.8)
P 		663.4
(-0.4)
P 		962.2
(3)
P 		613.8
(-1)
P 		653.8
(-0.5)
P 		433.6
(-3)
P 		529
B 510.5
(-2.1)
P 		577.3
(-1.5)
P 		1055.6
(3)
P 		633.5
(-1)
P 		599.4
(-1.3)
P 		416.4
(-3)
P 		639
C 375.5
(-3)
P 		624.3
(-0.5)
P 		970.7
(3)
P 		809.2
(1.4)
P 		617.3
(-0.6)
P 		466.9
(-2.1)
P 		595
avr 474.1
(-2.6) 		621.7
(-1) 		996.2
(3) 		685.5
(-0.3) 		623.5
(-1) 		439
(-3) 		557
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 201
1 2 3 4 range
A 260.8
(3)
P 		125.4
(-1)
P 		116.4
(-1.3)
P 		59.8
(-3)
P 		201
B 253.6
(3)
P 		130.7
(-1.3)
P 		132.1
(-1.3)
P 		83.3
(-3)
P 		170
C 250.3
(3)
P 		150.3
(-0.2)
P 		180.3
(0.8)
P 		63.5
(-3)
P 		187
avr 254.9
(3) 		135.5
(-0.9) 		142.9
(-0.6) 		68.9
(-3) 		186

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 98.9
(-3)
P 		235.9
(3)
P 		209.6
(1.8)
P 		155.7
(-0.5)
P 		197.3
(1.3)
P 		105.6
(-2.7)
P 		137
B 177.6
(-1.7)
P 		192.2
(-0.8)
P 		256.3
(3)
P 		167.8
(-2.3)
P 		243.5
(2.2)
P 		155.3
(-3)
P 		101
C 139.2
(-3)
P 		190.5
(-0.1)
P 		247.1
(3)
P 		162.7
(-1.7)
P 		199.7
(0.4)
P 		156.3
(-2)
P 		108
avr 138.6
(-3) 		206.2
(1.1) 		237.7
(3) 		162.1
(-1.6) 		213.5
(1.5) 		139.1
(-3) 		99
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188128_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14496
Sequence Type Exemplar sequence
Representative Public ID C50B6.7 NCBI
Target Description C50B6.7 /REP_DB=WormBase Gene ID /WP=CE08876 /TR=P91982 /GB=CAB02856.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=amylase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073902(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073902 NCBI Caenorhabditis elegans C50B6.7 (amylase) mRNA, complete cds. 11/11 None
SNAP00000011268 ENSEMBL cdna:SNAP chromosome:CEL140:V:13325016:13327463:-1 11/11 None
GENEFINDER00000011291 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13325016:13327562:-1 11/11 None
C50B6.7 ENSEMBL cdna:known chromosome:CEL140:V:13324971:13327562:-1 gene:C50B6.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13325015-13327562(-) 100.0 100.0
Public Domain and Genome References
Gene Title amylase
Ensembl C50B6.7
Entrez Gene 179813 Entrez gene
SwissProt P91982 EMBL-EBI
WormBase CE08876 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1591712_AT similar to Pancreatic alpha-amylase precursor (PA) (1,4-alpha-D-glucan glucanohydrolase) cfa
CANINE_2:CFAAFFX.30566.1.S1_AT similar to Pancreatic alpha-amylase precursor (PA) (1,4-alpha-D-glucan glucanohydrolase) cfa
CANINE_2:CFAAFFX.30566.1.S1_S_AT similar to Pancreatic alpha-amylase precursor (PA) (1,4-alpha-D-glucan glucanohydrolase) cfa
DROSOPHILA_2:1639374_S_AT Amylase proximal dm
DROSGENOME1:143068_AT Amylase proximal dm
DROSOPHILA_2:1639374_S_AT Amylase distal dm
DROSGENOME1:143068_AT Amylase distal dm
CHICKEN:GGA.19019.1.S1_S_AT amylase, alpha 2B; pancreatic gga
CHICKEN:GGA.4938.1.S1_S_AT amylase, alpha 2B; pancreatic gga
HG-U133_PLUS_2:208498_S_AT amylase, alpha 2A; pancreatic hs
HG-U133A:208498_S_AT amylase, alpha 2A; pancreatic hs
HG-FOCUS:208498_S_AT amylase, alpha 2A; pancreatic hs
HG-U133A_2:208498_S_AT amylase, alpha 2A; pancreatic hs
HG-U95AV2:36680_AT amylase, alpha 2A; pancreatic hs
U133_X3P:G4757749_3P_S_AT amylase, alpha 2A; pancreatic hs
HG-U133_PLUS_2:208498_S_AT amylase, alpha 2B; pancreatic hs
HG-U133A:208498_S_AT amylase, alpha 2B; pancreatic hs
HG-FOCUS:208498_S_AT amylase, alpha 2B; pancreatic hs
HG-U133A_2:208498_S_AT amylase, alpha 2B; pancreatic hs
HG-U95AV2:36680_AT amylase, alpha 2B; pancreatic hs
HUGENEFL:D90097_AT amylase, alpha 2B; pancreatic hs
U133_X3P:HS.278399.2.S1_3P_X_AT amylase, alpha 2B; pancreatic hs
U133_X3P:HS.278399.2.S1_3P_S_AT amylase, alpha 2B; pancreatic hs
HG-U133_PLUS_2:228023_X_AT amylase, alpha 2B; pancreatic hs
HG-U133B:228023_X_AT amylase, alpha 2B; pancreatic hs
HG-U95C:65391_AT amylase, alpha 2B; pancreatic hs
HG-U95D:89031_AT amylase, alpha 2B; pancreatic hs
HG-U95C:64340_AT Amylase, alpha 2B; pancreatic hs
HU35KSUBD:RC_R64129_AT Amylase, alpha 2B; pancreatic hs
MG-U74AV2:97524_F_AT amylase 2, pancreatic mm
MOE430A:1416055_AT amylase 2, pancreatic mm
MOUSE430A_2:1416055_AT amylase 2, pancreatic mm
MOUSE430_2:1416055_AT amylase 2, pancreatic mm
MU11KSUBB:MSA.427.0_F_AT amylase 2, pancreatic mm
MU11KSUBB:V00718_S_AT amylase 2, pancreatic mm
MU11KSUBB:V00719_S_AT amylase 2, pancreatic mm
MU11KSUBB:MSA.429.0_F_AT amylase 2, pancreatic mm
MG-U74AV2:97523_I_AT amylase 2, pancreatic mm
RG-U34A:V01225MRNA_S_AT similar to amylase 2, pancreatic rn
RAE230A:1369502_A_AT similar to amylase 2, pancreatic rn
RAT230_2:1369502_A_AT similar to amylase 2, pancreatic rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4556 alpha-amylase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02856 Hypothetical protein C50B6.7 [Caenorhabditis elegans] ref|NP_506303.1| C50B6.7 [Caenorhabditis elegans] 0.0
blast CAE62619 Hypothetical protein CBG06741 [Caenorhabditis briggsae] 0.0
blast XP_787209 PREDICTED: similar to alpha-amylase (5N804) [Strongylocentrotus purpuratus] 1.0E-134
Protein Domains
Database ID Description E-Value
Pfam IPR006048 EMBL-EBI Alpha amylase, C-terminal all-beta domain 2.8E-17
Pfam IPR006047 EMBL-EBI Alpha amylase, catalytic domain 9.0E-72
Pfam IPR006048 EMBL-EBI Alpha amylase, C-terminal all-beta domain 2.8E-17
Pfam IPR006047 EMBL-EBI Alpha amylase, catalytic domain 5.9E-69
Sequence
Target Sequence 
>C. ELEGANS:188128_AT
tggactctaaactcactccttatactccaccagcacctccatccggttacagtaccacgg
tgattcttctcaagaaagatacaaatcctggacagaatatttttgttagaggaggaacta
gtcaagctcataacggaaaatgctcaactggaccatatcaacagtcaagtgatccatgtg
caatcccgatgtatcacgcaacaactgtcccatttgtgtttgccgaatacttaacatgga
gccaacaagataattatctcgatttcgaaggagctgaaattaatcaaggaactcatgatg
gagccgctgcttttggaactccattggcatattccacaaatgacaaaacggcaattgaat
accagccatacaacaaatatgggccaggatattggattgccgttttaaggatggactgct
caaaagctgaacagggatggtttgaggttaagggatatgaaacaccggacattggatggg
aaggagatatcaagcagggatcatgcagtggatctgttggtggatctgctccattctctt
ctatcaaccacgtggcaaaatgcggagccgttaatgtattcacttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGACTCTAAACTCACTCCTTATAC 274 547 1535 Antisense
CCTCCATCCGGTTACAGTACCACGG 3 267 1570 Antisense
GTACCACGGTGATTCTTCTCAAGAA 25 459 1586 Antisense
GATCCATGTGCAATCCCGATGTATC 516 415 1705 Antisense
CGATGTATCACGCAACAACTGTCCC 36 249 1721 Antisense
GTCCCATTTGTGTTTGCCGAATACT 455 475 1741 Antisense
GAACTCATGATGGAGCCGCTGCTTT 250 349 1823 Antisense
GGAACTCCATTGGCATATTCCACAA 576 529 1849 Antisense
GATCTGTTGGTGGATCTGCTCCATT 387 419 2045 Antisense
CCATTCTCTTCTATCAACCACGTGG 493 269 2065 Antisense
GCGGAGCCGTTAATGTATTCACTTG 285 293 2096 Antisense
Sequence Source Affymetrix Proprietary Database