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[188120_at]

Description ZK1248.3 /REP_DB=WormBase Gene ID /WP=CE02903 /GEN=ehs-1 /TR=Q23418 /GB=AAC71084.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 03      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 41      6grp_total > 25      4grp_total > 54      6grp_highest_sub > De      6grp_cgc > 33      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 649
1 2 3 4 range
A 1183.8
(3)
P 		699.8
(-1.8)
P 		656.6
(-2.2)
P 		576.3
(-3)
P 		608
B 1068.4
(3)
P 		971.8
(1.4)
P 		772
(-1.9)
P 		703.5
(-3)
P 		365
C 1108
(3)
P 		716.5
(-1.1)
P 		799.7
(-0.2)
P 		534.7
(-3)
P 		573
avr 1120.1
(3) 		796
(-0.8) 		742.8
(-1.4) 		604.8
(-3) 		515

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 574
1 2 3 4 5 6 range
A 946.9
(1.7)
P 		1063.4
(3)
P 		639
(-1.9)
P 		540.3
(-3)
P 		573.2
(-2.6)
P 		609.2
(-2.2)
P 		523
B 1084
(3)
P 		779.4
(-0.3)
P 		735.1
(-0.8)
P 		527.5
(-3)
P 		538.1
(-2.9)
P 		587.1
(-2.4)
P 		557
C 980.4
(3)
P 		916.6
(2.2)
P 		894.5
(1.9)
P 		525.1
(-2.8)
P 		624.9
(-1.5)
P 		510.5
(-3)
P 		470
avr 1003.8
(3) 		919.8
(1.9) 		756.2
(-0.1) 		531
(-3) 		578.7
(-2.4) 		568.9
(-2.5) 		473
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 347
1 2 3 4 range
A 431
(3)
P 		151.9
(-1.8)
P 		171.6
(-1.5)
P 		84.4
(-3)
P 		347
B 340.7
(3)
P 		240
(-0.2)
P 		178.9
(-2.1)
P 		149.9
(-3)
P 		191
C 366.8
(3)
P 		206
(-0.5)
P 		278.7
(1.1)
P 		90.2
(-3)
P 		277
avr 379.5
(3) 		199.3
(-1) 		209.7
(-0.8) 		108.2
(-3) 		271

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 273
1 2 3 4 5 6 range
A 174.5
(-2.1)
P 		378.2
(3)
P 		139.2
(-2.9)
P 		137
(-3)
P 		173
(-2.1)
P 		148.3
(-2.7)
P 		241
B 377.2
(3)
P 		259.4
(0)
P 		178.5
(-2)
P 		139.7
(-3)
P 		218.6
(-1)
P 		218.9
(-1)
P 		238
C 363.4
(3)
P 		279.7
(1.1)
P 		227.7
(-0.2)
P 		105.6
(-3)
P 		202.2
(-0.8)
P 		170.9
(-1.5)
P 		258
avr 305
(3) 		305.8
(3) 		181.8
(-1.2) 		127.4
(-3) 		197.9
(-0.6) 		179.4
(-1.3) 		178
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188120_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4107
Sequence Type Exemplar sequence
Representative Public ID ZK1248.3 NCBI
Target Description ZK1248.3 /REP_DB=WormBase Gene ID /WP=CE02903 /GEN=ehs-1 /TR=Q23418 /GB=AAC71084.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027328(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027328 NCBI Caenorhabditis elegans Eps15 (endocytosis protein) Homologous Sequence family member (ehs-1) (ehs-1) mRNA, complete cds. 11/11 None
SNAP00000041375 ENSEMBL cdna:SNAP chromosome:CEL140:II:5820684:5823563:1 11/11 None
GENEFINDER00000041393 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5820684:5823563:1 11/11 None
ZK1248.3a ENSEMBL cdna:known chromosome:CEL140:II:5820676:5823827:1 gene:ZK1248.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062754 2/11 Cross Hyb Matching Probes None
ZK1248.3b 2/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173985 2 1 NM_001027328 188120_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5820863-5823560(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZK1248.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC71084 Eps15 (endocytosis protein) homologous sequence protein 1, isoform a [Caenorhabditis elegans] ref|NP_001022499.1| Eps15 (endocytosis protein) Homologous Sequence family member (ehs-1) [Caenorhabditis elegans] 0.0
blast AAK13051 EHS-1 [Caenorhabditis elegans] 0.0
blast AAN60507 Eps15 (endocytosis protein) homologous sequence protein 1, isoform b [Caenorhabditis elegans] ref|NP_495155.2| Eps15 (endocytosis protein) Homologous Sequence family member (ehs-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.071
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.071
Sequence
Target Sequence 
>C. ELEGANS:188120_AT
aaaggtggccgaattgacaactctagaacgaaaagaagcaagagatcagatacaaatgga
aaagctggatgctgcaatcgagaacacgacaaaactaacagaacaagtatcagatgcagt
cgaaaaatccgaggaggaaatggttcaaattctgaggtcgcaacaacgattattgtctac
tgtaatcgatcaatcgttgctatccgatgacactgtttatggagaaacagctggaacatc
atctcaaaatcatgttcaacaaccacctgatccgtttgcatccgccagagcaaatcctgc
tgctgatccattcgcccaagtagatcagttcggctcatctggacattttgatgccgcttt
cccaactgatccatttgctcaaggaggcttcccatcggattctggcttcgcacaatcggc
tcccgcaaaacctgctccacctcgcccagctcctccaaaatctgctcgcgaaacacctgt
caatgatccttttgccccatcacaaggacaatcaactcaaccggctggatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGGTGGCCGAATTGACAACTCTA 565 119 1701 Antisense
GTTGCTATCCGATGACACTGTTTAT 694 439 1896 Antisense
TCCGTTTGCATCCGCCAGAGCAAAT 406 595 1971 Antisense
AGAGCAAATCCTGCTGCTGATCCAT 402 69 1987 Antisense
GCTGATCCATTCGCCCAAGTAGATC 570 295 2002 Antisense
GTAGATCAGTTCGGCTCATCTGGAC 3 457 2020 Antisense
ATCTGGACATTTTGATGCCGCTTTC 111 35 2037 Antisense
CAAAATCTGCTCGCGAAACACCTGT 97 191 2156 Antisense
AACACCTGTCAATGATCCTTTTGCC 24 137 2172 Antisense
CTTTTGCCCCATCACAAGGACAATC 507 221 2189 Antisense
GACAATCAACTCAACCGGCTGGATT 283 365 2207 Antisense
Sequence Source Affymetrix Proprietary Database