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[188100_s_at]

Description F25H8.5A /REP_DB=WormBase Gene ID /WP=CE20722 /GEN=dur-1 /TR=Q19790 /GB=CAA93285.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 88      6grp_total > 97      4grp_total > 03      6grp_cgc > 69      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 888
1 2 3 4 range
A 110.9
(-2.6)
P 		97.2
(-2.7)
A 		56.1
(-3)
A 		918.9
(3)
P 		863
B 191
(-1.9)
P 		60.6
(-2.8)
A 		30.9
(-3)
A 		901.4
(3)
P 		871
C 234.7
(-1.3)
P 		50.4
(-2.9)
A 		40.5
(-3)
A 		707
(3)
P 		667
avr 178.9
(-2) 		69.4
(-2.8) 		42.5
(-3) 		842.4
(3) 		800

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1688
1 2 3 4 5 6 range
A 200.2
(-2.4)
P 		87.3
(-3)
A 		139.9
(-2.7)
P 		372.7
(-1.6)
P 		771.2
(0.4)
P 		1286.1
(3)
P 		1199
B 161.3
(-2.7)
P 		89.8
(-3)
P 		118.1
(-2.9)
P 		323.1
(-2.2)
P 		1062.1
(0.5)
P 		1756.6
(3)
P 		1667
C 133.4
(-2.7)
P 		143
(-2.7)
P 		68.6
(-3)
M 		270.6
(-2.1)
P 		999.6
(1)
P 		1481.1
(3)
P 		1413
avr 165
(-2.8) 		106.7
(-3) 		108.9
(-3) 		322.1
(-2.1) 		944.3
(0.6) 		1507.9
(3) 		1401
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 185
1 2 3 4 range
A 40.4
(-1.7)
P 		21.1
(-2.7)
A 		14.7
(-3)
A 		134.5
(3)
P 		120
B 60.9
(-1.3)
P 		15
(-2.7)
A 		7.2
(-3)
A 		192.1
(3)
P 		185
C 77.7
(0.6)
P 		14.5
(-3)
A 		14.1
(-3)
A 		119.2
(3)
P 		105
avr 59.7
(-0.9) 		16.9
(-2.8) 		12
(-3) 		148.6
(3) 		137

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 638
1 2 3 4 5 6 range
A 36.9
(-2.9)
P 		31.1
(-3)
A 		30.5
(-3)
P 		94.5
(-1.6)
P 		232.7
(1.3)
P 		313.1
(3)
P 		283
B 56.1
(-2.7)
P 		29.9
(-3)
P 		28.7
(-3)
P 		85.6
(-2.5)
P 		431.5
(0.9)
P 		655
(3)
P 		626
C 49.4
(-2.6)
P 		43.6
(-2.7)
P 		17.5
(-3)
M 		54.4
(-2.5)
P 		323.4
(0.8)
P 		495.8
(3)
P 		478
avr 47.5
(-2.7) 		34.9
(-2.9) 		25.6
(-3) 		78.2
(-2.3) 		329.2
(0.9) 		488
(3) 		462
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188100_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9358
Sequence Type Exemplar sequence
Representative Public ID F25H8.5A NCBI
Target Description F25H8.5A /REP_DB=WormBase Gene ID /WP=CE20722 /GEN=dur-1 /TR=Q19790 /GB=CAA93285.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069385(11), NM_001027974(11), NM_069386(11), U52071(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069385 NCBI Caenorhabditis elegans Dauer Up-Regulated family member (dur-1) (dur-1) mRNA, complete cds. 11/11 A 
NM_001027974 NCBI Caenorhabditis elegans Dauer Up-Regulated family member (dur-1) (dur-1) mRNA, complete cds. 11/11 None
NM_069386 NCBI Caenorhabditis elegans Dauer Up-Regulated family member (dur-1) (dur-1) mRNA, complete cds. 11/11 A 
U52071 NCBI Caenorhabditis elegans dauer-upregulated gene 135 (dur135) mRNA, partial cds. 10/11 None
SNAP00000013888 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9922023:9923717:-1 11/11 None
GENEFINDER00000013893 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9922023:9928194:-1 11/11 None
F25H8.5a ENSEMBL cdna:known chromosome:CEL140:IV:9921977:9928194:-1 gene:F25H8.5 11/11 A 
F25H8.5d ENSEMBL cdna:known chromosome:CEL140:IV:9922023:9928194:-1 gene:F25H8.5 11/11 A 
F25H8.5c ENSEMBL cdna:known chromosome:CEL140:IV:9922218:9928194:-1 gene:F25H8.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177846 4 3 NM_001027974 174446_s_at (11), 188100_s_at (11)
NM_069385 174446_s_at (11), 188100_s_at (11), 188104_s_at (11)
NM_069386 188100_s_at (11), 188104_s_at (11)
NM_069387 188104_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9922028-9928200(-) 100.0 100.0
Public Domain and Genome References
Ensembl F25H8.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA93285 Hypothetical protein F25H8.5a [Caenorhabditis elegans] ref|NP_501786.2| Dauer Up-Regulated family member (dur-1) [Caenorhabditis elegans] 0.0
blast CAC42291 Hypothetical protein F25H8.5c [Caenorhabditis elegans] ref|NP_501787.2| Dauer Up-Regulated family member (dur-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 4.4E-13
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 4.4E-13
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 4.4E-13
Sequence
Target Sequence 
>C. ELEGANS:188100_S_AT
gagcaggaaaggtagctgacggagcaaaatccgctggacaatcaattgccgatggagctg
ttgccgctaaagatgcagctgtcgaaggagcaaccacagccggtaacaagaccgtggaag
gaatgagatttgttggtgaaaatgtagctcacggagccggaaaggttgcagatggagcaa
aagctgctggagatacagtagttgaaggcgggaaagccgctgccgaaaaaactgaaagtg
gaattgcattcgtagggcaaaaaattgcggaaggcgctggagctgtagctggtgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCAGGAAAGGTAGCTGACGGAGC 131 389 1790 Antisense
ATCCGCTGGACAATCAATTGCCGAT 77 39 1818 Antisense
CCGATGGAGCTGTTGCCGCTAAAGA 22 265 1838 Antisense
GTTGCCGCTAAAGATGCAGCTGTCG 440 439 1849 Antisense
CCACAGCCGGTAACAAGACCGTGGA 422 271 1883 Antisense
GGTGAAAATGTAGCTCACGGAGCCG 286 501 1924 Antisense
CACGGAGCCGGAAAGGTTGCAGATG 274 199 1939 Antisense
TGAAGGCGGGAAAGCCGCTGCCGAA 393 573 1992 Antisense
AAGCCGCTGCCGAAAAAACTGAAAG 340 149 2003 Antisense
TGCGGAAGGCGCTGGAGCTGTAGCT 88 583 2055 Antisense
AGGCGCTGGAGCTGTAGCTGGTGGA 259 53 2061 Antisense
Sequence Source Affymetrix Proprietary Database