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[188096_s_at]

Description W03D2.5 /REP_DB=WormBase Gene ID /WP=CE14514 /GEN=wrt-5 /TR=O02116 /GB=AAC48258.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 91      4grp_total > 25      6grp_cgc > 97      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 249
1 2 3 4 range
A 223.5
(-3)
A 		407
(3)
A 		288.4
(-0.9)
A 		398.9
(2.7)
A 		184
B 173.6
(-3)
A 		290
(-0.2)
A 		309.7
(0.3)
A 		422.6
(3)
A 		249
C 217.3
(-3)
A 		296.5
(-0.1)
A 		286.1
(-0.5)
A 		381.8
(3)
A 		165
avr 204.8
(-3) 		331.2
(0.9) 		294.7
(-0.3) 		401.1
(3) 		196

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 282
1 2 3 4 5 6 range
A 329.3
(0.6)
A 		230.1
(-3)
A 		316.6
(0.2)
P 		349.2
(1.3)
A 		394.4
(3)
P 		274.3
(-1.4)
A 		164
B 269.6
(-3)
A 		411.4
(1)
P 		318.9
(-1.6)
P 		308.3
(-1.9)
P 		480.8
(3)
P 		321.3
(-1.5)
P 		211
C 303.7
(-0.4)
A 		319.7
(-0)
P 		198.7
(-3)
A 		444.8
(3)
P 		273.8
(-1.2)
A 		305.1
(-0.4)
P 		246
avr 300.9
(-1.7) 		320.4
(-0.6) 		278.1
(-3) 		367.4
(2.1) 		383
(3) 		300.2
(-1.7) 		105
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 81.4
(1.6)
A 		88.4
(3)
A 		75.4
(0.4)
A 		58.4
(-3)
A 		30
B 55.4
(-3)
A 		71.6
(-0.2)
A 		71.8
(-0.2)
A 		90.1
(3)
A 		35
C 71.9
(-1.7)
A 		85.2
(0.5)
A 		99.7
(3)
A 		64.4
(-3)
A 		35
avr 69.6
(-3) 		81.7
(2.7) 		82.3
(3) 		71
(-2.3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 145
1 2 3 4 5 6 range
A 60.7
(-3)
A 		81.8
(-0.8)
A 		69
(-2.1)
P 		88.6
(-0.1)
A 		119
(3)
P 		66.8
(-2.4)
A 		58
B 93.8
(-2.2)
A 		136.9
(0)
P 		77.4
(-3)
P 		81.7
(-2.8)
P 		195.3
(3)
P 		119.8
(-0.8)
P 		118
C 112.6
(3)
A 		97.6
(1.5)
P 		50.6
(-3)
A 		89.4
(0.8)
P 		88.6
(0.7)
A 		102.1
(2)
P 		62
avr 89
(-1) 		105.4
(0.5) 		65.7
(-3) 		86.6
(-1.2) 		134.3
(3) 		96.2
(-0.3) 		69
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188096_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11112
Sequence Type Exemplar sequence
Representative Public ID W03D2.5 NCBI
Target Description W03D2.5 /REP_DB=WormBase Gene ID /WP=CE14514 /GEN=wrt-5 /TR=O02116 /GB=AAC48258.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068066(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068066 NCBI Caenorhabditis elegans WaRThog (hedgehog-like family) family member (wrt-5) (wrt-5) mRNA, complete cds. 11/11 None
SNAP00000027037 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4048116:4051611:-1 11/11 None
GENEFINDER00000027049 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4050186:4051611:-1 11/11 None
W03D2.5 ENSEMBL cdna:known chromosome:CEL140:IV:4050097:4051624:-1 gene:W03D2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4050191-4051617(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol wrt-5
Ensembl W03D2.5
Entrez Gene 177162 Entrez gene
SwissProt O02116 EMBL-EBI
WormBase CE14514 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7154 cell communication inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5102 receptor binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAE67843 Hypothetical protein CBG13430 [Caenorhabditis briggsae] 1.0E-143
blast AAC48257 Pcna (proliferating cell nuclear antigen) homolog protein 1 [Caenorhabditis elegans] ref|NP_500466.2| W03D2.4 [Caenorhabditis elegans] sp|O02115|PCNA_CAEEL Proliferating cell nuclear antigen (PCNA) 1.0E-128
blast AAC48258 Warthog (hedgehog-like family) protein 5 [Caenorhabditis elegans] ref|NP_500467.1| WaRThog (hedgehog-like family) family member (wrt-5) [Caenorhabditis elegans] 1.0E-106
blast AAD33830 warthog protein WRT-5 [Caenorhabditis elegans] 1.0E-106
blast AAC48258 Warthog (hedgehog-like family) protein 5 [Caenorhabditis elegans] ref|NP_500467.1| WaRThog (hedgehog-like family) family member (wrt-5) [Caenorhabditis elegans] 1.0E-102
blast CAE73893 Hypothetical protein CBG21493 [Caenorhabditis briggsae] 7.0E-79
Protein Domains
Database ID Description E-Value
Pfam IPR000730 EMBL-EBI Proliferating cell nuclear antigen, PCNA 8.7E-61
Pfam IPR000730 EMBL-EBI Proliferating cell nuclear antigen, PCNA 7.2E-63
Sequence
Target Sequence 
>C. ELEGANS:188096_S_AT
gtgctccatgtggctcatggcttcatggctcatggcttttgtggccggctccactctcgc
agactactgtggggatcacaaggtgccatttggcatggaggtgcacaaaaatggcaatgt
gaacattttgtgctcgcggcccagttgtcacgagaagaagtatgcagaatgcccggaacg
agcgacttcaacgacatgctccaccaattcctcgtgggtcgggggagtaactcaacattc
tgacggaagtctgcggcttatgtgttgtgaatatgaccttcttcccacatattcaacgat
tcagtatgagaaacttactatccgcactggcgagtatttcgagggcgatgagcaaatgga
aggggatgttgtgacggcttttgacctgattggcaatattgagcaagtcaaagagcccga
tggaaaatactcctacaatctcctcatctaccgctatcattgcggaaatattcccgacac
accgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCTCCATGTGGCTCATGGCTTCA 513 479 15 Antisense
CATGGCTCATGGCTTTTGTGGCCGG 641 209 38 Antisense
CTCCACTCTCGCAGACTACTGTGGG 3 237 63 Antisense
GGAACGAGCGACTTCAACGACATGC 59 531 189 Antisense
GACGGAAGTCTGCGGCTTATGTGTT 48 377 256 Antisense
GTTGTGAATATGACCTTCTTCCCAC 446 437 278 Antisense
CTTCTTCCCACATATTCAACGATTC 459 221 292 Antisense
TACTATCCGCACTGGCGAGTATTTC 686 643 330 Antisense
GTGACGGCTTTTGACCTGATTGGCA 586 479 385 Antisense
GGAAAATACTCCTACAATCTCCTCA 22 529 436 Antisense
GCGGAAATATTCCCGACACACCGCC 535 293 476 Antisense
Sequence Source Affymetrix Proprietary Database