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[188095_at]

Description T14A8.1 /REP_DB=WormBase Gene ID /WP=CE27448 /GEN=ric-3 /TR=Q22472 /GB=AAA93437.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 35      4grp_highest_8_8 > 62      4grp_high_8_8 > 27      6grp_highest_nonorm > 70      6grp_total > 35      4grp_total > 37      6grp_highest_sub > 02      6grp_cgc > 44      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Cup      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 207
1 2 3 4 range
A 288.1
(0.2)
P 		336.9
(2.5)
P 		222.3
(-3)
P 		347.4
(3)
P 		125
B 401.5
(3)
P 		368.3
(2)
P 		209.4
(-3)
P 		242.2
(-2)
P 		192
C 416.3
(3)
P 		290
(-3)
P 		305
(-2.3)
P 		352.2
(-0)
P 		126
avr 368.6
(3) 		331.7
(1.2) 		245.6
(-3) 		313.9
(0.3) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 267
1 2 3 4 5 6 range
A 248.9
(-0.6)
P 		343.9
(1.9)
P 		384.8
(3)
P 		221
(-1.3)
P 		155.3
(-3)
P 		156.9
(-3)
P 		230
B 348.2
(1)
P 		412.9
(3)
P 		316.4
(0)
P 		218.8
(-3)
P 		263.1
(-1.6)
P 		283.9
(-1)
P 		194
C 362.5
(1.3)
P 		422.2
(3)
P 		297.2
(-0.5)
P 		266.8
(-1.4)
P 		209.1
(-3)
P 		281.9
(-1)
P 		213
avr 319.9
(0.6) 		393
(3) 		332.8
(1) 		235.5
(-2.1) 		209.2
(-3) 		240.9
(-2) 		184
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 104.9
(3)
P 		73.1
(-0.5)
P 		58.1
(-2.2)
P 		50.9
(-3)
P 		54
B 128
(3)
P 		91
(0.2)
P 		48.5
(-3)
P 		51.6
(-2.8)
P 		80
C 137.8
(3)
P 		83.4
(-1.2)
P 		106.3
(0.6)
P 		59.4
(-3)
P 		78
avr 123.6
(3) 		82.5
(-0.5) 		71
(-1.5) 		54
(-3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 45.9
(-2.5)
P 		122.3
(3)
P 		83.8
(0.3)
P 		56.1
(-1.7)
P 		46.9
(-2.4)
P 		38.2
(-3)
P 		84
B 121.2
(1.8)
P 		137.4
(3)
P 		76.8
(-1.6)
P 		58
(-3)
P 		106.9
(0.7)
P 		105.9
(0.6)
P 		79
C 134.4
(3)
P 		128.8
(2.6)
P 		75.6
(-1.4)
P 		53.7
(-3)
P 		67.7
(-2)
P 		94.4
(0)
P 		81
avr 100.5
(0.6) 		129.5
(3) 		78.7
(-1.1) 		55.9
(-3) 		73.8
(-1.5) 		79.5
(-1.1) 		74
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188095_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9679
Sequence Type Exemplar sequence
Representative Public ID T14A8.1 NCBI
Target Description T14A8.1 /REP_DB=WormBase Gene ID /WP=CE27448 /GEN=ric-3 /TR=Q22472 /GB=AAA93437.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068898(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068898 NCBI Caenorhabditis elegans Resistance to Inhibitors of Cholinesterase family member (ric-3) (ric-3) mRNA, complete cds. 11/11 None
SNAP00000022672 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7818246:7820729:1 11/11 None
GENEFINDER00000022674 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7818246:7820729:1 11/11 None
T14A8.1 ENSEMBL cdna:known chromosome:CEL140:IV:7818234:7821144:1 gene:T14A8.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7819191-7820735(+) 97.33 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ric-3
Ensembl T14A8.1
Entrez Gene 177570 Entrez gene
SwissProt Q22472 EMBL-EBI
WormBase CE27448 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA93437 Resistance to inhibitors of cholinesterase protein 3 [Caenorhabditis elegans] ref|NP_501299.1| Resistance to Inhibitors of Cholinesterase family member (ric-3) [Caenorhabditis elegans] 1.0E-104
blast CAE70892 Hypothetical protein CBG17683 [Caenorhabditis briggsae] 7.0E-81
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501299.1 2 44-66,125-147
T14A8.1 2 44-66,125-147
SNAP00000022672 2 44-66,125-147
GENEFINDER00000022674 2 44-66,125-147
Sequence
Target Sequence 
>C. ELEGANS:188095_AT
aggacttcagaaaaggcttcgagagactgaaagtgcaatgtctaagattttagaacaact
tgaatcagtacaagctggcgctaatcctgtcgacttagatgctgccgataagaggtcgga
acaacttgaagaagatccatcagttaaagaagcagttggattaactgaaaccaatgaaca
atacataaaagatcttgaggttgcactgaaagagtttcagtccttgtcaaaagaatatga
taaagcgaaaatgaagaaactgaaaagaaaagattcttcgagtgacgaagatgaagaaga
cgaagaagagaatagctctgaattgtcagaaatcgaagaggaagaagaggaagttaaacc
agtgaaaaagtcaaaatcatcctcccaatcagttggaaaaaggaagaatcgaccaaaaag
cacatcagaagaagaagatgaaggagaagaagaatcacgaaaagttgcagaagatgctga
agaagaaggaattgacattgattctgagatccgagagcacgcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGACTTCAGAAAAGGCTTCGAGAG 110 53 486 Antisense
AAGGCTTCGAGAGACTGAAAGTGCA 231 161 498 Antisense
TACAAGCTGGCGCTAATCCTGTCGA 354 641 554 Antisense
ATCCTGTCGACTTAGATGCTGCCGA 450 39 569 Antisense
GATGCTGCCGATAAGAGGTCGGAAC 161 415 583 Antisense
AAAAGATCTTGAGGTTGCACTGAAA 231 133 672 Antisense
AAAGAGTTTCAGTCCTTGTCAAAAG 97 121 694 Antisense
AAAAGTCAAAATCATCCTCCCAATC 402 131 851 Antisense
AATCATCCTCCCAATCAGTTGGAAA 581 169 860 Antisense
GAATCGACCAAAAAGCACATCAGAA 652 329 891 Antisense
TGATTCTGAGATCCGAGAGCACGCG 661 563 984 Antisense
Sequence Source Affymetrix Proprietary Database