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[188082_at]

Description T24D8.3 /REP_DB=WormBase Gene ID /WP=CE05007 /GEN=nlp-2 /TR=Q22737 /GB=AAA81444.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 26      4grp_total > 44      6grp_cgc > 14      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 30.6
(0.6)
A 		10.7
(-3)
A 		29.7
(0.4)
A 		43.9
(3)
A 		33
B 30.7
(-0.2)
A 		63.9
(3)
A 		6
(-2.7)
A 		2.5
(-3)
A 		61
C 14.8
(-2.5)
A 		15.8
(-2.2)
A 		31.7
(3)
A 		13.4
(-3)
A 		18
avr 25.4
(0.2) 		30.1
(3) 		22.5
(-1.5) 		19.9
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 38
1 2 3 4 5 6 range
A 2.7
(-3)
A 		10.2
(-0.7)
A 		15.6
(0.9)
A 		2.9
(-2.9)
A 		5.4
(-2.2)
A 		22.5
(3)
A 		20
B 24.8
(3)
A 		15.2
(0.3)
A 		3.6
(-2.9)
A 		3.1
(-3)
A 		3.6
(-2.9)
A 		3.6
(-2.9)
A 		22
C 41
(3)
A 		40.4
(2.9)
A 		3.9
(-2.9)
A 		32.1
(1.6)
A 		5.7
(-2.6)
A 		3.1
(-3)
A 		38
avr 22.8
(3) 		21.9
(2.7) 		7.7
(-2.1) 		12.7
(-0.4) 		4.9
(-3) 		9.7
(-1.4) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 11.2
(3)
A 		2.3
(-3)
A 		7.8
(0.7)
A 		6.4
(-0.2)
A 		9
B 9.8
(0.6)
A 		15.8
(3)
A 		1.4
(-2.6)
A 		0.5
(-3)
A 		15
C 4.9
(-1.2)
A 		4.6
(-1.4)
A 		11
(3)
A 		2.3
(-3)
A 		9
avr 8.6
(3) 		7.6
(1.9) 		6.7
(0.9) 		3.1
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 0.5
(-3)
A 		3.6
(0.7)
A 		3.4
(0.5)
A 		0.7
(-2.8)
A 		1.6
(-1.7)
A 		5.5
(3)
A 		5
B 8.6
(3)
A 		5
(0.2)
A 		0.9
(-2.9)
A 		0.8
(-3)
A 		1.4
(-2.5)
A 		1.4
(-2.5)
A 		8
C 15.2
(3)
A 		12.3
(1.8)
A 		1
(-3)
A 		6.4
(-0.7)
A 		1.9
(-2.6)
A 		1
(-3)
A 		14
avr 8.1
(3) 		7
(2) 		1.8
(-2.8) 		2.6
(-2.1) 		1.6
(-3) 		2.6
(-2.1) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188082_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18117
Sequence Type Exemplar sequence
Representative Public ID T24D8.3 NCBI
Target Description T24D8.3 /REP_DB=WormBase Gene ID /WP=CE05007 /GEN=nlp-2 /TR=Q22737 /GB=AAA81444.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076023(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076023 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-22) (nlp-22) mRNA, complete cds. 11/11 None
SNAP00000017733 ENSEMBL cdna:SNAP chromosome:CEL140:X:2341352:2342057:-1 11/11 None
GENEFINDER00000017745 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2341352:2342057:-1 11/11 None
T24D8.3 ENSEMBL cdna:known chromosome:CEL140:X:2341352:2342057:-1 gene:T24D8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2341351-2342057(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-22
Ensembl T24D8.3
Entrez Gene 188855 Entrez gene
SwissProt Q22737 EMBL-EBI
WormBase CE05007 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81444 Neuropeptide-like protein protein 22 [Caenorhabditis elegans] ref|NP_508424.1| Neuropeptide-Like Protein family member (nlp-22) [Caenorhabditis elegans] 1.0E-51
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508424.1 1 5-27
T24D8.3 1 5-27
SNAP00000017733 1 5-27
GENEFINDER00000017745 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:188082_AT
tgcgttccataatcgtcttcatcggattgacgatcttcgcgttggacattcttctggtcc
agacctcagctcttgggcttcagggtggaatcgatgtcttccgcggattaggagttgtcg
atcaagtcgatttcaatcagatccttcaccgagcaaactacctgcgcaacactcgtgagg
ggcgcttgcgctattggagactccgaacccttccaatcatgaaaaagtcgattgcgattg
ggcgagccggattcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCGTTCCATAATCGTCTTCATCGG 234 583 14 Antisense
GACGATCTTCGCGTTGGACATTCTT 636 377 42 Antisense
CATTCTTCTGGTCCAGACCTCAGCT 447 215 60 Antisense
CTCAGCTCTTGGGCTTCAGGGTGGA 566 227 78 Antisense
TGGAATCGATGTCTTCCGCGGATTA 459 545 99 Antisense
GTCGATTTCAATCAGATCCTTCACC 580 473 139 Antisense
TTCACCGAGCAAACTACCTGCGCAA 216 685 158 Antisense
GCGCAACACTCGTGAGGGGCGCTTG 645 279 177 Antisense
CGCTTGCGCTATTGGAGACTCCGAA 29 253 196 Antisense
CTCCGAACCCTTCCAATCATGAAAA 150 235 214 Antisense
ATTGCGATTGGGCGAGCCGGATTCC 571 5 244 Antisense
Sequence Source Affymetrix Proprietary Database