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[188081_at]

Description C05H8.1 /REP_DB=WormBase Gene ID /WP=CE01146 /GEN=ckk-1 /TR=Q17682 /GB=AAA19242.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 24      4grp_highest_8_8 > 52      4grp_high_8_8 > 25      6grp_highest_nonorm > 70      6grp_total > 12      4grp_total > 39      6grp_highest_sub > 02      6grp_cgc > 43      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 295
1 2 3 4 range
A 401.5
(3)
P 		154.1
(-3)
P 		207.9
(-1.7)
P 		205
(-1.8)
P 		247
B 431.9
(3)
P 		243.1
(-1.2)
P 		165.2
(-3)
P 		272.3
(-0.6)
P 		267
C 449.3
(3)
P 		229.5
(-2.1)
P 		188.9
(-3)
P 		302.5
(-0.4)
P 		260
avr 427.6
(3) 		208.9
(-2.5) 		187.3
(-3) 		259.9
(-1.2) 		240

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 274
1 2 3 4 5 6 range
A 328.2
(0.6)
P 		418.8
(3)
P 		254.4
(-1.4)
P 		218.5
(-2.3)
P 		235.5
(-1.9)
P 		192.8
(-3)
P 		226
B 314.1
(1)
P 		377.2
(3)
P 		268.5
(-0.4)
P 		258.2
(-0.7)
P 		215.9
(-2)
P 		184.4
(-3)
P 		193
C 406
(2.5)
P 		428.7
(3)
P 		298.8
(0.2)
P 		224.6
(-1.5)
P 		220.1
(-1.6)
P 		154.8
(-3)
P 		274
avr 349.4
(1.5) 		408.2
(3) 		273.9
(-0.5) 		233.8
(-1.5) 		223.8
(-1.8) 		177.3
(-3) 		231
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 146.2
(3)
P 		33.4
(-2.8)
P 		54.4
(-1.7)
P 		30
(-3)
P 		116
B 137.7
(3)
P 		60
(-1.7)
P 		38.3
(-3)
P 		58
(-1.8)
P 		99
C 148.7
(3)
P 		66
(-2.1)
P 		65.8
(-2.1)
P 		51
(-3)
P 		98
avr 144.2
(3) 		53.1
(-2.6) 		52.8
(-2.6) 		46.3
(-3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 60.5
(-2.2)
P 		148.9
(3)
P 		55.4
(-2.5)
P 		55.4
(-2.5)
P 		71.1
(-1.6)
P 		47
(-3)
P 		102
B 109.3
(1.4)
P 		125.6
(3)
P 		65.2
(-3)
P 		68.4
(-2.7)
P 		87.7
(-0.8)
P 		68.8
(-2.6)
P 		60
C 150.5
(3)
P 		130.8
(1.9)
P 		76.1
(-1.2)
P 		45.2
(-3)
P 		71.2
(-1.5)
P 		51.8
(-2.6)
P 		105
avr 106.8
(0.9) 		135.1
(3) 		65.6
(-2.3) 		56.3
(-3) 		76.7
(-1.4) 		55.9
(-3) 		79
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188081_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6929
Sequence Type Exemplar sequence
Representative Public ID C05H8.1 NCBI
Target Description C05H8.1 /REP_DB=WormBase Gene ID /WP=CE01146 /GEN=ckk-1 /TR=Q17682 /GB=AAA19242.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025982(11), NM_001025981(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001025982 NCBI Caenorhabditis elegans CaM Kinase Kinase family member (ckk-1) (ckk-1) mRNA, complete cds. 11/11 None
NM_001025981 NCBI Caenorhabditis elegans CaM Kinase Kinase family member (ckk-1) (ckk-1) mRNA, complete cds. 11/11 None
C05H8.1b ENSEMBL cdna:known chromosome:CEL140:III:5635429:5654485:1 gene:C05H8.1 11/11 None
C05H8.1a ENSEMBL cdna:known chromosome:CEL140:III:5651493:5654508:1 gene:C05H8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5651691-5653559(+) 100.0 100.0
Public Domain and Genome References
Ensembl C05H8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001021153 CaM Kinase Kinase family member (ckk-1) [Caenorhabditis elegans] 0.0
blast AAZ82849 Cam kinase kinase protein 1, isoform b [Caenorhabditis elegans] 0.0
blast AAA19242 Cam kinase kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001021152.1| CaM Kinase Kinase family member (ckk-1) [Caenorhabditis elegans] dbj|BAA77824.4| Ca2+/calmodulin-dependent protein kinase kinase [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 6.0E-60
hanks 2.2.12 CaMK Group; CaMK II KIN1/SNF1/Nim1; YCL24 2.0E-63
hanks 2.2.12 CaMK Group; CaMK II KIN1/SNF1/Nim1; YCL24 2.0E-64
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 5.2E-73
Pfam IPR000719 EMBL-EBI Protein kinase 5.2E-73
Sequence
Target Sequence 
>C. ELEGANS:188081_AT
acatcaagccgtcaaaccttctgctcagcgatataggtcaagtaaaaattgccgatttcg
gagtgtcttgtgaatttgaaggaatcgacgcatttctatcgggaactgccggtacgcctg
ccttcatggctccagaagcattgacggaaggggcaaatcatttttattcgggtcgagcac
aggatatctggtcattaggaatcactttatacgcgtttgtaattggaactgtcccattcg
ttgataattacatcatagccctccacaaaaaaatcaaaaacgatccgattgttttcccgg
aagcaccaattttaagtgaagctctgcaagacattattctaggaatgctcaaaaaagatc
ccgggcatcgattaatgttgcacgaagtgaaagtacacacgtgggtgacacgtgacggta
ccgtaccaatgtcatcagaacaggagaattgtcatctggttactgtaacggaggaagaaa
tcgagaattgcgtcagagtgataccacggttggatactctaattctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACATCAAGCCGTCAAACCTTCTGCT 411 103 512 Antisense
GACGCATTTCTATCGGGAACTGCCG 544 379 598 Antisense
CTGCCTTCATGGCTCCAGAAGCATT 220 237 629 Antisense
ATTCGGGTCGAGCACAGGATATCTG 304 7 677 Antisense
AATTGGAACTGTCCCATTCGTTGAT 577 177 732 Antisense
ATAATTACATCATAGCCCTCCACAA 675 23 755 Antisense
GATCCGATTGTTTTCCCGGAAGCAC 5 417 793 Antisense
AAGATCCCGGGCATCGATTAATGTT 49 155 866 Antisense
GTGGGTGACACGTGACGGTACCGTA 313 487 912 Antisense
CGGTACCGTACCAATGTCATCAGAA 650 243 927 Antisense
ACCACGGTTGGATACTCTAATTCTG 235 85 1014 Antisense
Sequence Source Affymetrix Proprietary Database