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[188080_s_at]

Description F56A3.4 /REP_DB=WormBase Gene ID /WP=CE11220 /GEN=spd-5 /TR=P91349 /GB=AAB37802.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 96      6grp_highest_nonorm > 96      6grp_total > 86      4grp_total > 60      6grp_highest_sub > In      6grp_cgc > 58      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 498
1 2 3 4 range
A 593
(3)
P 		472.9
(1)
P 		528.3
(1.9)
P 		236.6
(-3)
A 		356
B 501.9
(0.8)
P 		685.4
(3)
P 		665.3
(2.8)
P 		187.5
(-3)
A 		498
C 618.6
(3)
P 		460.6
(0.8)
P 		606.3
(2.8)
P 		192.3
(-3)
A 		426
avr 571.2
(2.6) 		539.6
(2.1) 		600
(3) 		205.5
(-3) 		395

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1272
1 2 3 4 5 6 range
A 476.3
(-2.5)
P 		719.9
(-1.3)
P 		362
(-3)
P 		962.4
(-0.2)
P 		814.2
(-0.9)
P 		1634.4
(3)
P 		1272
B 542.1
(-2.7)
P 		505.1
(-3)
P 		632.1
(-2)
P 		814.3
(-0.5)
P 		855.3
(-0.2)
P 		1249.9
(3)
P 		745
C 488.9
(-3)
P 		687.9
(-1.5)
P 		648.2
(-1.8)
P 		1044.4
(1.2)
P 		725.2
(-1.2)
P 		1285.7
(3)
P 		797
avr 502.4
(-3) 		637.6
(-2.1) 		547.4
(-2.7) 		940.4
(-0) 		798.2
(-1) 		1390
(3) 		888
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 215.9
(3)
P 		102.7
(-0.7)
P 		138.1
(0.4)
P 		34.6
(-3)
A 		181
B 160.1
(2.6)
P 		169.3
(3)
P 		154.2
(2.3)
P 		40
(-3)
A 		129
C 204.8
(2.8)
P 		132.4
(0.4)
P 		211.3
(3)
P 		32.4
(-3)
A 		179
avr 193.6
(3) 		134.8
(0.8) 		167.9
(2) 		35.7
(-3) 		158

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 387
1 2 3 4 5 6 range
A 87.8
(-2.8)
P 		256
(0.3)
P 		78.8
(-3)
P 		244.1
(0.1)
P 		245.7
(0.1)
P 		397.9
(3)
P 		319
B 188.6
(-2.3)
P 		168.1
(-2.7)
P 		153.5
(-3)
P 		215.7
(-1.8)
P 		347.5
(0.7)
P 		466.1
(3)
P 		313
C 181.2
(-2.6)
P 		209.9
(-2)
P 		165
(-3)
P 		210
(-2)
P 		234.7
(-1.4)
P 		430.4
(3)
P 		265
avr 152.5
(-2.6) 		211.3
(-1.4) 		132.4
(-3) 		223.3
(-1.2) 		276
(-0.1) 		431.5
(3) 		299
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188080_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1045
Sequence Type Exemplar sequence
Representative Public ID F56A3.4 NCBI
Target Description F56A3.4 /REP_DB=WormBase Gene ID /WP=CE11220 /GEN=spd-5 /TR=P91349 /GB=AAB37802.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059138(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059138 NCBI Caenorhabditis elegans SPindle Defective family member (spd-5) (spd-5) mRNA, complete cds. 11/11 None
GENEFINDER00000016717 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5171146:5175524:-1 11/11 None
SNAP00000016706 ENSEMBL cdna:SNAP chromosome:CEL140:I:5171497:5173897:-1 11/11 None
F56A3.4 ENSEMBL cdna:known chromosome:CEL140:I:5171286:5175525:-1 gene:F56A3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5171496-5175524(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spd-5
Ensembl F56A3.4
Entrez Gene 172155 Entrez gene
SwissProt P91349 EMBL-EBI
WormBase CE28834 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37802 Spindle defective protein 5 [Caenorhabditis elegans] ref|NP_491539.1| SPindle Defective family member (spd-5) [Caenorhabditis elegans] sp|P91349|SPD5_CAEEL Spindle-defective protein 0.0
blast CAE56131 Hypothetical protein CBG23743 [Caenorhabditis briggsae] 0.0
blast XP_473881 OSJNBa0008M17.5 [Oryza sativa (japonica cultivar-group)] emb|CAE04334.2| OSJNBa0008M17.5 [Oryza sativa (japonica cultivar-group)] 4.0E-30
Sequence
Target Sequence 
>C. ELEGANS:188080_S_AT
tccagggaacgagagcaacagcatgaatacgcgaaagttatgagagacgtcaatgaaaaa
attgaaaagcttcaattagagaaagatgctctcgaacatgagctgaaaatgatgagctct
aataacgagaatgtcccaccagttggaacatcggtcagtggcatgcctactaaaacatcg
aatcaaaagtgtgctcaacctcattacacgagccctactcgacaattgctccacgaatca
actatggctgtcgacgcaattgttcagaaactcaagaagactcacaatatgtcaggaatg
ggtccagagcttaaggagacgattggcaacgtcatcaatgaatccagagttcttcgcgat
ttcctccatcagaagctcattctattcaaaggaatcgatatgtccaattggaagaacgag
accgtcgatcaactgatcacagatctcggacaattgcatcaggataacttgatgcttgag
gagcaaatcaagaagtacaaaaaggaactgaaactgacaaaatccgccatccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCAGGGAACGAGAGCAACAGCATG 305 295 2983 Antisense
ATAACGAGAATGTCCCACCAGTTGG 467 25 3104 Antisense
GTCCCACCAGTTGGAACATCGGTCA 284 475 3115 Antisense
ACATCGGTCAGTGGCATGCCTACTA 297 105 3130 Antisense
ATTACACGAGCCCTACTCGACAATT 584 11 3185 Antisense
CCTACTCGACAATTGCTCCACGAAT 428 265 3196 Antisense
GCTCCACGAATCAACTATGGCTGTC 11 299 3210 Antisense
CTATGGCTGTCGACGCAATTGTTCA 334 225 3224 Antisense
AGACGATTGGCAACGTCATCAATGA 196 71 3299 Antisense
GAACGAGACCGTCGATCAACTGATC 390 345 3396 Antisense
TGAAACTGACAAAATCCGCCATCCC 434 573 3491 Antisense
Sequence Source Affymetrix Proprietary Database