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[188078_at] lipase

Description F46B6.8 /REP_DB=WormBase Gene ID /WP=CE05874 /TR=Q20449 /GB=CAA94824.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=lipase
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 42      4grp_highest_8_8 > 60      4grp_high_8_8 > 25      6grp_highest_nonorm > 43      6grp_total > 52      4grp_total > 39      6grp_highest_sub > 03      6grp_high1_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 433
1 2 3 4 range
A 799
(3)
P 		459.8
(-2.3)
P 		411.9
(-3)
P 		420.5
(-2.9)
P 		387
B 727
(3)
P 		495.5
(-0.8)
P 		366.2
(-3)
P 		611.2
(1.1)
P 		361
C 757.4
(3)
P 		501.4
(-3)
P 		512.1
(-2.7)
P 		599
(-0.7)
P 		256
avr 761.1
(3) 		485.6
(-2) 		430.1
(-3) 		543.6
(-0.9) 		331

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1929
1 2 3 4 5 6 range
A 681.7
(-2.2)
P 		1360.9
(-0.1)
P 		2357.4
(3)
P 		1204
(-0.6)
P 		745
(-2)
P 		428.3
(-3)
P 		1929
B 629.7
(-2.2)
P 		1366
(0.9)
P 		1883.9
(3)
P 		950.4
(-0.9)
P 		828.2
(-1.4)
P 		434.2
(-3)
P 		1450
C 688.9
(-2.4)
P 		1434.2
(1.4)
P 		1741
(3)
P 		965.3
(-1)
P 		714.5
(-2.2)
P 		564.7
(-3)
P 		1176
avr 666.8
(-2.2) 		1387
(0.6) 		1994.1
(3) 		1039.9
(-0.8) 		762.6
(-1.9) 		475.7
(-3) 		1518
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 229
1 2 3 4 range
A 290.9
(3)
P 		99.8
(-2)
P 		107.7
(-1.8)
P 		61.5
(-3)
P 		229
B 231.8
(3)
P 		122.4
(-1.5)
P 		84.8
(-3)
P 		130.2
(-1.1)
P 		147
C 250.7
(3)
P 		144.2
(-1.3)
P 		178.5
(0.1)
P 		101
(-3)
P 		150
avr 257.8
(3) 		122.1
(-2.1) 		123.7
(-2) 		97.6
(-3) 		160

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 409
1 2 3 4 5 6 range
A 125.7
(-2.7)
P 		484
(2.6)
P 		513.4
(3)
P 		305.4
(-0.1)
P 		224.8
(-1.2)
P 		104.3
(-3)
P 		409
B 219.1
(-1.8)
P 		454.7
(2.9)
P 		457.5
(3)
P 		251.7
(-1.2)
P 		336.5
(0.5)
P 		161.9
(-3)
P 		296
C 255.3
(-1.4)
P 		437.6
(2.9)
P 		443.1
(3)
P 		194.1
(-2.9)
P 		231.2
(-2)
P 		189
(-3)
P 		254
avr 200
(-2.1) 		458.8
(2.8) 		471.3
(3) 		250.4
(-1.1) 		264.2
(-0.9) 		151.7
(-3) 		320
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188078_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11877
Sequence Type Exemplar sequence
Representative Public ID F46B6.8 NCBI
Target Description F46B6.8 /REP_DB=WormBase Gene ID /WP=CE05874 /TR=Q20449 /GB=CAA94824.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=lipase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073126(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073126 NCBI Caenorhabditis elegans F46B6.8 (lipase) mRNA, complete cds. 11/11 None
SNAP00000041200 ENSEMBL cdna:SNAP chromosome:CEL140:V:9794170:9795803:1 11/11 None
GENEFINDER00000041211 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9794170:9797688:1 11/11 None
F46B6.8 ENSEMBL cdna:known chromosome:CEL140:V:9794170:9795803:1 gene:F46B6.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9794169-9795803(+) 99.19 100.0
Public Domain and Genome References
Gene Title lipase
Ensembl F46B6.8
Entrez Gene 185840 Entrez gene
SwissProt Q20449 EMBL-EBI
WormBase CE05874 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.3733.1.S1_S_AT lipase, gastric cfa
DROSOPHILA_2:1636583_AT dm
DROSGENOME1:141426_AT dm
HG-U95AV2:35395_AT lipase, gastric hs
HUGENEFL:X05997_AT lipase, gastric hs
HG-U133A:206334_AT lipase, gastric hs
HG-U133_PLUS_2:206334_AT lipase, gastric hs
HG-FOCUS:206334_AT lipase, gastric hs
HG-U133A_2:206334_AT lipase, gastric hs
U133_X3P:G4758675_3P_AT lipase, gastric hs
MOE430A:1416040_AT lipase, gastric mm
MOUSE430A_2:1416040_AT lipase, gastric mm
MOUSE430_2:1416040_AT lipase, gastric mm
MG-U74BV2:162544_I_AT lipase, gastric mm
RG-U34A:A01157CDS_S_AT lipase, gastric rn
RG-U34A:X02309_AT lipase, gastric rn
RAE230A:1387245_AT lipase, gastric rn
RAT230_2:1387245_AT lipase, gastric rn
YEAST_2:1769848_AT Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6629 lipid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
16789 carboxylic ester hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94824 Hypothetical protein F46B6.8 [Caenorhabditis elegans] ref|NP_505527.1| F46B6.8 [Caenorhabditis elegans] 0.0
blast CAE72256 Hypothetical protein CBG19375 [Caenorhabditis briggsae] 0.0
blast AAG45574 Hypothetical protein ZK6.7a [Caenorhabditis elegans] ref|NP_503184.1| ZK6.7a [Caenorhabditis elegans] 1.0E-161
Protein Domains
Database ID Description E-Value
Pfam IPR006693 EMBL-EBI Ab-hydrolase associated lipase region 1.6E-46
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000041211 1 423-445
Sequence
Target Sequence 
>C. ELEGANS:188078_AT
caaggggttcttgtcctattttgcgcacaagttctctcccgaatttgatggttggtacga
gctgttcggttccaaagacttccttcctgacaattggattacaaaaatggcagcaaaaga
catttgtggagcatcagaaaaagaggctgaactctgtgataatgaactctttctaattgg
tggaccagagagtgatcagtggaatgcatcgagaactgcaatttacagttcacaggatcc
agcaggaacttctactcaaaatattgtacactggatgcagatggttcgcaatggaagagt
tccagcatttgattggggaaagaaaataaataagaagaagtacggacaagatacaccacc
agaatatgatttcggagccatcaagggcaccaaaattcatttgtattggagtgacgatga
ttggcttggagatccaacagatattcatgatttccttttgaaggaactgaatccggctgt
aattgctgaaaatgtcaatttgaaagaatacaatcacttggatttcacatggggact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGGGGTTCTTGTCCTATTTTGCG 13 157 642 Antisense
TCCTATTTTGCGCACAAGTTCTCTC 507 591 655 Antisense
AAGTTCTCTCCCGAATTTGATGGTT 105 157 670 Antisense
GGTTGGTACGAGCTGTTCGGTTCCA 251 355 691 Antisense
TTCCAAAGACTTCCTTCCTGACAAT 274 699 711 Antisense
GAACTCTTTCTAATTGGTGGACCAG 537 349 805 Antisense
ATTTACAGTTCACAGGATCCAGCAG 317 15 862 Antisense
GTACGGACAAGATACACCACCAGAA 640 455 981 Antisense
GATTTCGGAGCCATCAAGGGCACCA 67 427 1009 Antisense
GGAACTGAATCCGGCTGTAATTGCT 691 529 1104 Antisense
ATCACTTGGATTTCACATGGGGACT 566 27 1154 Antisense
Sequence Source Affymetrix Proprietary Database