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[188068_at]

Description F37A8.4 /REP_DB=WormBase Gene ID /WP=CE00989 /GEN=nlp-10 /TR=Q20114 /GB=CAA84664.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 26      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 76      6grp_total > 25      4grp_total > 13      6grp_highest_sub > De      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3048
1 2 3 4 range
A 1980
(1.5)
P 		922.5
(-1.6)
P 		458
(-3)
P 		2504.3
(3)
P 		2046
B 2282.3
(1.2)
P 		707.4
(-2.5)
P 		497.6
(-3)
P 		3061.2
(3)
P 		2564
C 2067.4
(0.1)
P 		798.9
(-2.5)
P 		535.6
(-3)
P 		3505.6
(3)
P 		2970
avr 2109.9
(0.8) 		809.6
(-2.3) 		497.1
(-3) 		3023.7
(3) 		2527

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 411
1 2 3 4 5 6 range
A 1201.1
(0.3)
P 		1301.3
(3)
P 		1228.3
(1)
P 		1122.2
(-1.8)
P 		1077.3
(-3)
P 		1181.9
(-0.2)
P 		224
B 1288.7
(1.5)
P 		1351.4
(3)
P 		1280.9
(1.3)
P 		1098
(-3)
P 		1190.9
(-0.8)
P 		1139.6
(-2)
P 		253
C 1405.8
(3)
P 		1199.5
(-0)
P 		994.9
(-3)
P 		1244.8
(0.6)
P 		1023
(-2.6)
P 		1120.3
(-1.2)
P 		411
avr 1298.5
(3) 		1284.1
(2.6) 		1168
(-0.9) 		1155
(-1.3) 		1097.1
(-3) 		1147.3
(-1.5) 		201
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 613
1 2 3 4 range
A 720.9
(3)
P 		200.3
(-2.2)
P 		119.7
(-3)
P 		366.6
(-0.5)
P 		601
B 727.8
(3)
P 		174.7
(-2.4)
P 		115.3
(-3)
P 		652.3
(2.3)
P 		613
C 684.4
(3)
P 		229.7
(-2.5)
P 		186.7
(-3)
P 		591.1
(1.9)
P 		498
avr 711
(3) 		201.6
(-2.4) 		140.6
(-3) 		536.7
(1.2) 		570

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 300
1 2 3 4 5 6 range
A 221.4
(-3)
P 		462.8
(3)
P 		267.5
(-1.9)
P 		284.6
(-1.4)
P 		325.1
(-0.4)
P 		287.8
(-1.3)
P 		241
B 448.4
(1.9)
P 		449.8
(1.9)
P 		311
(-2.4)
P 		290.8
(-3)
P 		483.9
(3)
P 		425
(1.2)
P 		193
C 521.1
(3)
P 		366
(-0.4)
P 		253.2
(-2.9)
P 		250.3
(-3)
P 		331
(-1.2)
P 		375
(-0.2)
P 		271
avr 397
(1.8) 		426.2
(3) 		277.2
(-2.9) 		275.2
(-3) 		380
(1.2) 		362.6
(0.5) 		151
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188068_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7961
Sequence Type Exemplar sequence
Representative Public ID F37A8.4 NCBI
Target Description F37A8.4 /REP_DB=WormBase Gene ID /WP=CE00989 /GEN=nlp-10 /TR=Q20114 /GB=CAA84664.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065394(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065394 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-10) (nlp-10) mRNA, complete cds. 11/11 None
SNAP00000009909 ENSEMBL cdna:SNAP chromosome:CEL140:III:3933755:3935840:-1 11/11 None
GENEFINDER00000009916 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3933755:3935840:-1 11/11 None
F37A8.4 ENSEMBL cdna:known chromosome:CEL140:III:3933600:3935883:-1 gene:F37A8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3933754-3935840(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-10
Ensembl F37A8.4
Entrez Gene 191733 Entrez gene
SwissProt Q20114 EMBL-EBI
WormBase CE00989 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA84664 Hypothetical protein F37A8.4 [Caenorhabditis elegans] ref|NP_497795.1| Neuropeptide-Like Protein family member (nlp-10) [Caenorhabditis elegans] 6.0E-63
blast CAE60111 Hypothetical protein CBG03649 [Caenorhabditis briggsae] 9.0E-34
Sequence
Target Sequence 
>C. ELEGANS:188068_AT
cacctcggtcacagcacagaaagcagacgacgagccaatcgtcttcctggttcgagttcc
cattgatgaaatggacgatgattcaagtcttctcgagtcatactatcatccaagagatat
attatcaaaacgagcgattccattcaacggcggtatgtacggcaaacgatcaacaatgcc
gttctcaggtggaatgtacggaaagagatccggacagatttttgctcaacgaagagccgc
tattccattttctgggggaatgtatggaaagcgttcgttggtgccacaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACCTCGGTCACAGCACAGAAAGCA 425 201 48 Antisense
ACAGAAAGCAGACGACGAGCCAATC 655 105 63 Antisense
TTCCTGGTTCGAGTTCCCATTGATG 54 699 91 Antisense
GGACGATGATTCAAGTCTTCTCGAG 513 523 120 Antisense
GTCTTCTCGAGTCATACTATCATCC 209 469 134 Antisense
AAACGAGCGATTCCATTCAACGGCG 466 123 175 Antisense
AACGGCGGTATGTACGGCAAACGAT 470 135 193 Antisense
ACGATCAACAATGCCGTTCTCAGGT 410 93 213 Antisense
ATCCGGACAGATTTTTGCTCAACGA 523 37 255 Antisense
ACGAAGAGCCGCTATTCCATTTTCT 236 91 276 Antisense
GAAAGCGTTCGTTGGTGCCACAAAG 33 359 314 Antisense
Sequence Source Affymetrix Proprietary Database