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[188067_at]

Description C37C3.4 /REP_DB=WormBase Gene ID /WP=CE06929 /GEN=grd-8 /TR=Q22914 /GB=AAC25861.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 26      6grp_cgc > 88      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 10.8
(-3)
A 		58.2
(1.9)
A 		14.6
(-2.6)
A 		68.4
(3)
A 		58
B 14
(0.4)
A 		8.2
(-3)
A 		12.6
(-0.4)
A 		18.4
(3)
A 		10
C 33.5
(2.3)
A 		22.3
(-1.4)
A 		35.6
(3)
A 		17.3
(-3)
A 		18
avr 19.4
(-3) 		29.6
(1) 		20.9
(-2.4) 		34.7
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 113
1 2 3 4 5 6 range
A 35
(-1.6)
A 		23.4
(-2.2)
A 		8.5
(-3)
A 		37.6
(-1.4)
A 		120.9
(3)
A 		39.3
(-1.4)
A 		112
B 10.5
(-2.8)
A 		9.6
(-2.9)
A 		21.4
(-1.8)
A 		11.8
(-2.7)
A 		72.4
(3)
A 		8.4
(-3)
A 		64
C 9.9
(-3)
A 		10.3
(-3)
A 		12.9
(-2.8)
A 		24.5
(-1.8)
A 		85.7
(3)
A 		36.4
(-0.9)
A 		76
avr 18.5
(-2.7) 		14.4
(-3) 		14.3
(-3) 		24.6
(-2.2) 		93
(3) 		28
(-2) 		79
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 3.9
(-2.9)
A 		12.6
(3)
A 		3.8
(-3)
A 		10
(1.2)
A 		9
B 4.5
(3)
A 		2
(-3)
A 		2.9
(-0.8)
A 		3.9
(1.6)
A 		3
C 11.1
(2.2)
A 		6.4
(-0.8)
A 		12.4
(3)
A 		2.9
(-3)
A 		10
avr 6.5
(0.9) 		7
(3) 		6.4
(0.4) 		5.6
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 6.4
(-2.2)
A 		8.3
(-1.9)
A 		1.9
(-3)
A 		9.5
(-1.7)
A 		36.5
(3)
A 		9.6
(-1.7)
A 		35
B 3.7
(-2.9)
A 		3.2
(-3)
A 		5.2
(-2.5)
A 		3.1
(-3)
A 		29.4
(3)
A 		3.1
(-3)
A 		26
C 3.7
(-2.9)
A 		3.1
(-3)
A 		3.3
(-3)
A 		4.9
(-2.6)
A 		27.7
(3)
A 		12.2
(-0.8)
A 		25
avr 4.6
(-2.8) 		4.9
(-2.7) 		3.5
(-3) 		5.8
(-2.5) 		31.2
(3) 		8.3
(-2) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188067_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11660
Sequence Type Exemplar sequence
Representative Public ID C37C3.4 NCBI
Target Description C37C3.4 /REP_DB=WormBase Gene ID /WP=CE06929 /GEN=grd-8 /TR=Q22914 /GB=AAC25861.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072619(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072619 NCBI Caenorhabditis elegans GRounDhog (hedgehog-like family) family member (grd-8) (grd-8) mRNA, complete cds. 11/11 None
SNAP00000006077 ENSEMBL cdna:SNAP chromosome:CEL140:V:7845253:7846891:1 11/11 None
GENEFINDER00000006096 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7843029:7846891:1 11/11 None
C37C3.4 ENSEMBL cdna:known chromosome:CEL140:V:7845135:7846891:1 gene:C37C3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7845810-7846892(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grd-8
Ensembl C37C3.4
Entrez Gene 191662 Entrez gene
SwissProt Q22914 EMBL-EBI
WormBase CE34270 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC25868 Hypothetical protein C37C3.6b [Caenorhabditis elegans] ref|NP_505017.1| PaPiliN (Drosophila ECM protein) homolog family member (ppn-1) [Caenorhabditis elegans] 0.0
blast CAE73170 Hypothetical protein CBG20566 [Caenorhabditis briggsae] 1.0E-161
blast AAC25861 Groundhog (hedgehog-like family) protein 8 [Caenorhabditis elegans] ref|NP_505020.2| GRounDhog (hedgehog-like family) family member (grd-8) [Caenorhabditis elegans] 1.0E-155
blast AAC25861 Groundhog (hedgehog-like family) protein 8 [Caenorhabditis elegans] ref|NP_505020.2| GRounDhog (hedgehog-like family) family member (grd-8) [Caenorhabditis elegans] 1.0E-124
blast CAE73169 Hypothetical protein CBG20565 [Caenorhabditis briggsae] 1.0E-62
blast CAE73169 Hypothetical protein CBG20565 [Caenorhabditis briggsae] 2.0E-62
blast AAA83334 Groundhog (hedgehog-like family) protein 6 [Caenorhabditis elegans] ref|NP_505389.3| GRounDhog (hedgehog-like family) family member (grd-6) [Caenorhabditis elegans] 4.0E-20
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 6.1E-28
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 6.1E-28
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 6.1E-28
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 8.1E-20
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 1.8E-28
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 2.5E-27
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 7.3E-8
Sequence
Target Sequence 
>C. ELEGANS:188067_AT
aaaggtcatacctatacggctctgggcggtggacaattctatcaaagtttacttggaggg
aaaggaggccccggaggattctccccactctcgttctttctaaacggcggtctaggaggt
actggtggtggtggtaacaatggattcttcgttccggtgcctgtagtcattccgcctcca
ccgccaccgccaccaggaccaaactgtttcacgaacccgtcgggattcctttgctgtaac
gtgacacttgagaaaactatggaagacgcgtacctggccgcaaaagcagatggtgcatca
ctgtgcaatgtacagaaaatggcaactgcagtgcaagcgcaagccgaaaaaaaattcgga
acaactttcgaatcagtcgctgctcattcggacttcgtcgcaaaaattaattttgccggt
gacctgaactgtaaaatagaaatcgatgggaaattcatactagcgtacgcaactccaatc
gccgagcaagaggtgaacattgtcgatgctagctcattcttctcgggagctgctgata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGGTCATACCTATACGGCTCTGG 382 119 277 Antisense
CCACTCTCGTTCTTTCTAAACGGCG 302 271 361 Antisense
AACGGCGGTCTAGGAGGTACTGGTG 572 79 379 Antisense
AACAATGGATTCTTCGTTCCGGTGC 144 135 412 Antisense
GCCACCAGGACCAAACTGTTTCACG 318 279 465 Antisense
GGGATTCCTTTGCTGTAACGTGACA 557 495 498 Antisense
AACTTTCGAATCAGTCGCTGCTCAT 647 139 639 Antisense
TGCTCATTCGGACTTCGTCGCAAAA 439 581 657 Antisense
GCAACTCCAATCGCCGAGCAAGAGG 63 319 745 Antisense
ATTGTCGATGCTAGCTCATTCTTCT 337 5 775 Antisense
TCATTCTTCTCGGGAGCTGCTGATA 538 619 790 Antisense
Sequence Source Affymetrix Proprietary Database