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[188058_at]

Description K09F5.3 /REP_DB=WormBase Gene ID /WP=CE12022 /GEN=uvt-1 /TR=O17943 /GB=AAB52676.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 12      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 06      6grp_total > 23      4grp_total > 53      6grp_highest_sub > 01_02_03      6grp_cgc > 61      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21313
1 2 3 4 range
A 22434.5
(3)
P 		11337.2
(-0.1)
P 		11839.3
(0)
P 		1170.2
(-3)
P 		21264
B 17605.7
(3)
P 		16643.3
(2.6)
P 		10977.1
(0.6)
P 		1170.7
(-3)
P 		16435
C 18125.1
(2.3)
P 		20515.4
(3)
P 		13911.4
(1)
P 		1121.8
(-3)
P 		19394
avr 19388.4
(3) 		16165.3
(1.9) 		12242.6
(0.6) 		1154.2
(-3) 		18234

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12975
1 2 3 4 5 6 range
A 8106.9
(0.1)
P 		10111
(1.2)
P 		13573.4
(3)
P 		5548.5
(-1.2)
P 		4347.7
(-1.8)
P 		2070.7
(-3)
P 		11503
B 13831.5
(2.5)
P 		14600.8
(2.8)
P 		14969.7
(3)
P 		5708.4
(-1.3)
P 		4256.4
(-2)
P 		2074.3
(-3)
P 		12895
C 15045.4
(3)
P 		12401
(1.8)
P 		7306.6
(-0.6)
P 		8037.2
(-0.3)
P 		3859.6
(-2.3)
P 		2322.8
(-3)
P 		12723
avr 12327.9
(3) 		12370.9
(3) 		11949.9
(2.8) 		6431.4
(-0.5) 		4154.6
(-1.8) 		2155.9
(-3) 		10215
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7997
1 2 3 4 range
A 8168.4
(3)
P 		2461
(-1.3)
P 		3095
(-0.8)
P 		171.3
(-3)
P 		7997
B 5614.3
(3)
P 		4110.5
(1.3)
P 		2543.7
(-0.4)
P 		249.5
(-3)
P 		5365
C 5999.9
(3)
P 		5898.3
(2.9)
P 		4848.5
(1.8)
P 		189.2
(-3)
P 		5811
avr 6594.2
(3) 		4156.6
(0.7) 		3495.7
(0.1) 		203.3
(-3) 		6391

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5073
1 2 3 4 5 6 range
A 1494.4
(-1.1)
P 		3596
(3)
P 		2956
(1.8)
P 		1407.2
(-1.2)
P 		1311.9
(-1.4)
P 		504.2
(-3)
P 		3092
B 4812.7
(2.9)
P 		4860
(3)
P 		3635.1
(1.2)
P 		1512
(-1.9)
P 		1729.3
(-1.6)
P 		773.5
(-3)
P 		4087
C 5576.7
(3)
P 		3783.9
(0.8)
P 		1859.7
(-1.6)
P 		1616.3
(-2)
P 		1248.9
(-2.4)
P 		777.6
(-3)
P 		4799
avr 3961.3
(2.8) 		4080
(3) 		2816.9
(0.8) 		1511.8
(-1.5) 		1430
(-1.7) 		685.1
(-3) 		3395
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188058_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17142
Sequence Type Exemplar sequence
Representative Public ID K09F5.3 NCBI
Target Description K09F5.3 /REP_DB=WormBase Gene ID /WP=CE12022 /GEN=uvt-1 /TR=O17943 /GB=AAB52676.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076902(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076902 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-14) (spp-14) mRNA, complete cds. 11/11 None
K09F5.3a ENSEMBL cdna:known chromosome:CEL140:X:7719211:7726333:1 gene:K09F5.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029610 5/11 Cross Hyb Matching Probes None
SNAP00000026490 5/11 Cross Hyb Matching Probes None
GENEFINDER00000026499 5/11 Cross Hyb Matching Probes None
SNAP00000026491 5/11 Cross Hyb Matching Probes None
GENEFINDER00000026496 5/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
181032 3 1 NM_076902 188058_at (11)
NM_076903 175222_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7719420-7726278(+) 100.0 100.0
Public Domain and Genome References
Ensembl K09F5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_509303 SaPosin-like Protein family member (spp-14) [Caenorhabditis elegans] gb|AAB52676.1| Saposin-like protein family protein 14, isoform a [Caenorhabditis elegans] 0.0
blast CAE70262 Hypothetical protein CBG16766 [Caenorhabditis briggsae] 0.0
blast NP_509304 SaPosin-like Protein family member (spp-14) [Caenorhabditis elegans] gb|AAL08045.2| Saposin-like protein family protein 14, isoform b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 1.3E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509303.1 7 12-34,54-76,219-238,248-270,302-324,380-402,409-431
K09F5.3a 7 12-34,54-76,219-238,248-270,302-324,380-402,409-431
Sequence
Target Sequence 
>C. ELEGANS:188058_AT
ttaccccgacttcaacactaacaagtcacggaagtaactacactccaacacctggatctc
cacaaggatccaatccgatctattcccgaatcaatggagcatcttccacatcgtcaaatc
tttacggagttgcttccaaagaaagtcaatatgctgacaggacacttcaaaaagcaccga
tcaaatctacaacagtgacgactggaaatacaatacttcgttccgtgaacggaacagatg
acactgtatcaagcttttctcgtccacccactggagcacttaaccaaaatgactttgcta
ccagcattgtaatgaagaccaccttctgccttctggcccttgtcgccgtcgctaccgctt
tggctctcccaagccaacaggacaatgccatgagctgcctcatgtgcgaggtcggagtcc
gtgctgctgaaaacccagctgacagagaagctcacaccgttgaggacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTACCCCGACTTCAACACTAACAAG 390 663 2459 Antisense
GTCACGGAAGTAACTACACTCCAAC 557 463 2483 Antisense
TCCAACACCTGGATCTCCACAAGGA 572 587 2502 Antisense
AGGATCCAATCCGATCTATTCCCGA 275 53 2523 Antisense
ACACTGTATCAAGCTTTTCTCGTCC 80 109 2699 Antisense
GTAATGAAGACCACCTTCTGCCTTC 390 461 2767 Antisense
CTTTGGCTCTCCCAAGCCAACAGGA 489 221 2816 Antisense
CAGGACAATGCCATGAGCTGCCTCA 629 207 2836 Antisense
TCATGTGCGAGGTCGGAGTCCGTGC 547 621 2858 Antisense
AGTCCGTGCTGCTGAAAACCCAGCT 510 59 2874 Antisense
GAGAAGCTCACACCGTTGAGGACAA 395 391 2903 Antisense
Sequence Source Affymetrix Proprietary Database