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[188046_s_at]

Description F57F4.3 /REP_DB=WormBase Gene ID /WP=CE11342 /GEN=gfi-1 /TR=Q94246 /GB=AAB09166.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 03      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 04      6grp_total > 24      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5228
1 2 3 4 range
A 5554.1
(3)
P 		4834.2
(2.2)
P 		3487.3
(0.6)
P 		344
(-3)
P 		5210
B 5571.9
(3)
P 		4745
(2)
P 		3782.3
(0.9)
P 		498
(-3)
P 		5074
C 5106.9
(3)
P 		4606.2
(2.4)
P 		3365.9
(0.8)
P 		444.3
(-3)
P 		4663
avr 5411
(3) 		4728.5
(2.2) 		3545.2
(0.8) 		428.8
(-3) 		4982

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5623
1 2 3 4 5 6 range
A 7254.8
(3)
P 		5677.3
(1.2)
P 		3643.8
(-1.1)
P 		2560.9
(-2.3)
P 		2291.9
(-2.6)
P 		1907.3
(-3)
P 		5348
B 6325.8
(3)
P 		5677.7
(2.2)
P 		4978.1
(1.3)
P 		3034.9
(-1.2)
P 		1796.4
(-2.8)
P 		1679.8
(-3)
P 		4646
C 5906.2
(3)
P 		5427.7
(2.3)
P 		4940.6
(1.6)
P 		2954.6
(-1.1)
P 		2204.6
(-2.2)
P 		1632.3
(-3)
P 		4274
avr 6495.6
(3) 		5594.2
(1.9) 		4520.8
(0.5) 		2850.1
(-1.6) 		2097.6
(-2.5) 		1739.8
(-3) 		4756
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1972
1 2 3 4 range
A 2022.2
(3)
P 		1049.4
(0)
P 		911.6
(-0.4)
P 		50.3
(-3)
P 		1972
B 1776.8
(3)
P 		1171.9
(0.8)
P 		876.5
(-0.2)
P 		106.1
(-3)
P 		1671
C 1690.5
(3)
P 		1324.3
(1.6)
P 		1173.1
(1.1)
P 		74.9
(-3)
P 		1616
avr 1829.8
(3) 		1181.9
(0.8) 		987.1
(0.1) 		77.1
(-3) 		1753

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1737
1 2 3 4 5 6 range
A 1337.4
(0.4)
P 		2019.1
(3)
P 		793.5
(-1.7)
P 		649.5
(-2.3)
P 		691.6
(-2.1)
P 		464.4
(-3)
P 		1555
B 2201.1
(3)
P 		1889.9
(1.8)
P 		1208.8
(-0.8)
P 		803.8
(-2.3)
P 		729.9
(-2.6)
P 		626.4
(-3)
P 		1575
C 2189.2
(3)
P 		1656.1
(1.1)
P 		1257.5
(-0.4)
P 		594.2
(-2.8)
P 		713.4
(-2.4)
P 		546.4
(-3)
P 		1643
avr 1909.2
(3) 		1855
(2.8) 		1086.6
(-0.6) 		682.5
(-2.4) 		711.6
(-2.3) 		545.7
(-3) 		1364
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188046_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11428
Sequence Type Exemplar sequence
Representative Public ID F57F4.3 NCBI
Target Description F57F4.3 /REP_DB=WormBase Gene ID /WP=CE11342 /GEN=gfi-1 /TR=Q94246 /GB=AAB09166.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072225(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072225 NCBI Caenorhabditis elegans GEI-4(Four) Interacting protein family member (gfi-1) (gfi-1) mRNA, complete cds. 11/11 None
SNAP00000021868 ENSEMBL cdna:SNAP chromosome:CEL140:V:6395292:6402515:-1 11/11 None
GENEFINDER00000021872 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6395292:6402515:-1 11/11 None
F57F4.3 ENSEMBL cdna:known chromosome:CEL140:V:6395130:6402515:-1 gene:F57F4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6395291-6402515(-) 100.0 100.0
chrV:6404124-6411377(-) 95.31 95.31
Public Domain and Genome References
Gene Title
Gene Symbol gfi-1
Ensembl F57F4.3
Entrez Gene 179022 Entrez gene
SwissProt Q94246 EMBL-EBI
WormBase CE11342 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB09166 Gei-4(four) interacting protein protein 1 [Caenorhabditis elegans] ref|NP_504626.1| GEI-4(Four) Interacting protein family member (gfi-1) [Caenorhabditis elegans] 0.0
blast AAB09167 Hypothetical protein F57F4.4 [Caenorhabditis elegans] ref|NP_504627.1| F57F4.4 [Caenorhabditis elegans] 0.0
blast CAE71872 Hypothetical protein CBG18927 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 3.3E-21
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 4.4E-26
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 1.0E-35
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 2.8E-29
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 3.6E-41
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 3.2E-37
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 3.8E-41
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 2.5E-37
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 3.3E-40
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 2.9E-37
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 3.3E-42
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 5.7E-41
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 6.1E-44
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 1.2E-38
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 1.3E-42
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 1.3E-36
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 1.7E-44
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 4.4E-38
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 1.2E-38
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 1.2E-33
Pfam IPR002603 EMBL-EBI Protein of unknown function ET 3.5E-36
Sequence
Target Sequence 
>C. ELEGANS:188046_S_AT
ataacatcgctctgacagctgacgcctacagtgtatgcgctggatcatgtgcgtctattt
catacactggcccatacaacggaggaagtcagacactgacattgtacacatgtgatccag
ttactgtctgtacctccttgaacttggccaactcgtgtgcttccattgacggtggcctct
ctggatgctgttgtagcactaataactgcgttggtccaaacgtgctgccaagcgccggca
ccacacagccatcaactgtttccacattgtcaccaattgtttccacattgtcaccaattg
tttccacattgtcaccaattgtttccacattgtcaccaattgtttccacattgtcaccaa
ctgtttccactctgtcatcaaatgctacctcgctgacaccaagtaatcctgtattggtat
caactgctactacacagtcgccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATAACATCGCTCTGACAGCTGACGC 101 25 5945 Antisense
AGCTGACGCCTACAGTGTATGCGCT 94 77 5961 Antisense
GTGCGTCTATTTCATACACTGGCCC 279 477 5993 Antisense
TACACTGGCCCATACAACGGAGGAA 532 641 6007 Antisense
GCACCACACAGCCATCAACTGTTTC 412 315 6182 Antisense
AACTGTTTCCACATTGTCACCAATT 402 143 6198 Antisense
ATTGTTTCCACATTGTCACCAATTG 521 3 6220 Antisense
TCCACATTGTCACCAATTGTTTCCA 311 589 6268 Antisense
TCCACATTGTCACCAACTGTTTCCA 310 589 6289 Antisense
CTACCTCGCTGACACCAAGTAATCC 522 225 6329 Antisense
TCAACTGCTACTACACAGTCGCCAG 411 631 6364 Antisense
Sequence Source Affymetrix Proprietary Database