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[188044_s_at]

Description K10D3.2 /REP_DB=WormBase Gene ID /WP=CE25055 /GEN=unc-14 /TR=O15940 /GB=CAB61025.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 13      4grp_highest_8_8 > 61      4grp_high_8_8 > 17      6grp_highest_nonorm > 21      6grp_total > 14      4grp_total > 30      6grp_highest_sub > De      6grp_cgc > 30      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1217
1 2 3 4 range
A 1796.5
(3)
P 		1077.2
(-0.9)
P 		694.1
(-3)
P 		1398.5
(0.8)
P 		1102
B 1825.1
(3)
P 		929.3
(-1.4)
P 		608
(-3)
P 		1341.4
(0.6)
P 		1217
C 1742.4
(3)
P 		948.1
(-1.8)
P 		750.7
(-3)
P 		1084.3
(-1)
P 		992
avr 1788
(3) 		984.9
(-1.4) 		684.3
(-3) 		1274.7
(0.2) 		1104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1136
1 2 3 4 5 6 range
A 1747.2
(2.1)
P 		1889.4
(3)
P 		1100.2
(-1.8)
P 		1333.5
(-0.4)
P 		1034.7
(-2.2)
P 		910.8
(-3)
P 		979
B 1742
(3)
P 		1433.4
(1)
P 		1337.2
(0.3)
P 		1268.8
(-0.1)
P 		830.7
(-3)
P 		884.2
(-2.6)
P 		911
C 1751.4
(3)
P 		1501.6
(1.5)
P 		1496.5
(1.5)
P 		1185.4
(-0.4)
P 		994.5
(-1.6)
P 		753.3
(-3)
P 		998
avr 1746.9
(3) 		1608.1
(2.1) 		1311.3
(0.1) 		1262.6
(-0.2) 		953.3
(-2.3) 		849.4
(-3) 		898
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 513
1 2 3 4 range
A 654.1
(3)
P 		233.8
(-2.3)
P 		181.5
(-3)
P 		204.7
(-2.7)
P 		473
B 582
(3)
P 		229.5
(-1.8)
P 		140.9
(-3)
P 		285.9
(-1)
P 		441
C 576.8
(3)
P 		272.6
(-1.6)
P 		261.6
(-1.8)
P 		182.8
(-3)
P 		394
avr 604.3
(3) 		245.3
(-2.3) 		194.7
(-3) 		224.5
(-2.6) 		410

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 450
1 2 3 4 5 6 range
A 322.1
(-1.7)
P 		672
(3)
P 		239.6
(-2.8)
P 		338.2
(-1.4)
P 		312.2
(-1.8)
P 		221.8
(-3)
P 		450
B 606.1
(3)
P 		477.1
(0.2)
P 		324.7
(-3)
P 		336.1
(-2.8)
P 		337.5
(-2.7)
P 		329.7
(-2.9)
P 		281
C 649.2
(3)
P 		458.2
(0.2)
P 		380.9
(-0.9)
P 		238.4
(-3)
P 		321.8
(-1.8)
P 		252.2
(-2.8)
P 		411
avr 525.8
(2.8) 		535.8
(3) 		315.1
(-1.9) 		304.2
(-2.2) 		323.8
(-1.7) 		267.9
(-3) 		268
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188044_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.588
Sequence Type Exemplar sequence
Representative Public ID K10D3.2 NCBI
Target Description K10D3.2 /REP_DB=WormBase Gene ID /WP=CE25055 /GEN=unc-14 /TR=O15940 /GB=CAB61025.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059617(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059617 NCBI Caenorhabditis elegans UNCoordinated family member (unc-14) (unc-14) mRNA, complete cds. 11/11 A 
SNAP00000021172 ENSEMBL cdna:SNAP chromosome:CEL140:I:7131192:7134717:-1 11/11 None
GENEFINDER00000021183 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7131192:7134717:-1 11/11 None
K10D3.2 ENSEMBL cdna:known chromosome:CEL140:I:7130744:7136555:-1 gene:K10D3.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7131122-7136200(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol unc-14
Ensembl K10D3.2
Entrez Gene 172450 Entrez gene
SwissProt O15940 EMBL-EBI
WormBase CE25055 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB61025 Hypothetical protein K10D3.2 [Caenorhabditis elegans] ref|NP_492018.1| UNCoordinated family member (unc-14) [Caenorhabditis elegans] dbj|BAA21715.1| UNC-14 [Caenorhabditis elegans] 0.0
blast CAE74238 Hypothetical protein CBG21923 [Caenorhabditis briggsae] 0.0
blast T23571 hypothetical protein K10D3.2 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004012 EMBL-EBI RUN domain 3.1E-37
Pfam IPR004012 EMBL-EBI RUN domain 3.1E-37
Sequence
Target Sequence 
>C. ELEGANS:188044_S_AT
aaaactcattggactgctggctgtgctatattgtgttgaaagagaaggtgctgaaaacat
tgtattctgaaaatgcattccttctttctgcgagttctgaatacagaactcttctttgga
gaatggtcgacagtttgtctcttttgccagtgatcgaagctcgcagtgattcagttcacc
aacagttcaaatcaatgcaacaatggggcggagcatccagaatagcttctgactcaagag
ttccaaagagttcatcgttcccagcaagattatcaactgctccatcaagaagatcaagga
taccattgtcaacaagtcgcatcagtatcagctcaacaacttcaacacctcgatcagctc
gatcaccttcaacgacatctcgtattcgagttgcatctataatgggtgacttcacacttg
ccaatttctccctctcagacggagaaaaagttagtgttctatcaactcgtggaggacttg
ctcgatgtgtccgtttaacgacttctcattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAACTCATTGGACTGCTGGCTGTG 200 131 1451 Antisense
GCTGGCTGTGCTATATTGTGTTGAA 286 295 1466 Antisense
AAAATGCATTCCTTCTTTCTGCGAG 575 133 1520 Antisense
TTTGTCTCTTTTGCCAGTGATCGAA 340 667 1584 Antisense
TCCAAAGAGTTCATCGTTCCCAGCA 18 589 1692 Antisense
AAGTCGCATCAGTATCAGCTCAACA 658 157 1764 Antisense
TTCAACACCTCGATCAGCTCGATCA 604 685 1791 Antisense
ATGGGTGACTTCACACTTGCCAATT 136 49 1852 Antisense
CCAATTTCTCCCTCTCAGACGGAGA 285 275 1871 Antisense
GAGGACTTGCTCGATGTGTCCGTTT 301 403 1922 Antisense
GTGTCCGTTTAACGACTTCTCATTC 160 483 1937 Antisense
Sequence Source Affymetrix Proprietary Database