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[188037_at]

Description ZK377.1 /REP_DB=WormBase Gene ID /WP=CE15329 /GEN=wrt-6 /TR=P91573 /GB=AAB52656.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 90      6grp_total > 92      4grp_total > 49      6grp_cgc > 64      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 379
1 2 3 4 range
A 95.1
(-3)
A 		423.9
(2.8)
P 		432.5
(3)
P 		101
(-2.9)
A 		337
B 65.5
(-2.8)
A 		378.5
(3)
P 		359.6
(2.7)
P 		54
(-3)
A 		325
C 80.5
(-3)
A 		361.9
(2.1)
P 		413.5
(3)
P 		92.9
(-2.8)
A 		333
avr 80.4
(-3) 		388.1
(2.7) 		401.9
(3) 		82.6
(-3) 		322

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 463
1 2 3 4 5 6 range
A 140.4
(-2.8)
A 		536.5
(3)
P 		196.5
(-2)
P 		341.4
(0.1)
P 		526.7
(2.9)
P 		126.9
(-3)
M 		410
B 157.5
(-2.6)
P 		500.2
(2.2)
P 		198.4
(-2.1)
P 		286.9
(-0.8)
P 		560.8
(3)
P 		130.3
(-3)
P 		431
C 116.8
(-2.9)
P 		475.4
(1.8)
P 		254.5
(-1.1)
P 		292.2
(-0.6)
P 		568.9
(3)
P 		106.3
(-3)
P 		463
avr 138.2
(-2.8) 		504
(2.3) 		216.5
(-1.7) 		306.8
(-0.4) 		552.1
(3) 		121.2
(-3) 		431
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 133
1 2 3 4 range
A 34.6
(-1.8)
A 		92
(1.7)
P 		113.1
(3)
P 		14.8
(-3)
A 		98
B 20.9
(-2.3)
A 		93.5
(3)
P 		83.3
(2.3)
P 		11.5
(-3)
A 		82
C 26.7
(-2.5)
A 		104
(1.1)
P 		144.1
(3)
P 		15.7
(-3)
A 		128
avr 27.4
(-2.2) 		96.5
(2) 		113.5
(3) 		14
(-3) 		100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 202
1 2 3 4 5 6 range
A 25.9
(-3)
A 		190.8
(3)
P 		42.8
(-2.4)
P 		86.6
(-0.8)
P 		158.9
(1.8)
P 		30.9
(-2.8)
M 		165
B 54.8
(-2.8)
P 		166.5
(0.9)
P 		48.2
(-3)
P 		76
(-2.1)
P 		227.9
(3)
P 		48.6
(-3)
P 		180
C 43.3
(-2.7)
P 		145.1
(1.4)
P 		64.8
(-1.8)
P 		58.8
(-2.1)
P 		184.1
(3)
P 		35.6
(-3)
P 		149
avr 41.3
(-2.9) 		167.5
(2.1) 		51.9
(-2.5) 		73.8
(-1.6) 		190.3
(3) 		38.4
(-3) 		152
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188037_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18399
Sequence Type Exemplar sequence
Representative Public ID ZK377.1 NCBI
Target Description ZK377.1 /REP_DB=WormBase Gene ID /WP=CE15329 /GEN=wrt-6 /TR=P91573 /GB=AAB52656.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076196(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076196 NCBI Caenorhabditis elegans WaRThog (hedgehog-like family) family member (wrt-6) (wrt-6) mRNA, complete cds. 11/11 None
GENEFINDER00000036070 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3480241:3483470:1 11/11 None
SNAP00000036068 ENSEMBL cdna:SNAP chromosome:CEL140:X:3481488:3483470:1 11/11 None
ZK377.1 ENSEMBL cdna:known chromosome:CEL140:X:3480238:3484031:1 gene:ZK377.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3480241-3483471(+) 95.67 100.0
Public Domain and Genome References
Gene Title
Gene Symbol wrt-6
Ensembl ZK377.1
Entrez Gene 180638 Entrez gene
SwissProt P91573 EMBL-EBI
WormBase CE37175 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
7154 cell communication inferred from electronic annotation QuickGO AmiGO
7275 development inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8233 peptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52656 Warthog (hedgehog-like family) protein 6 [Caenorhabditis elegans] ref|NP_508597.2| WaRThog (hedgehog-like family) family member (wrt-6) [Caenorhabditis elegans] 0.0
blast CAE68437 Hypothetical protein CBG14222 [Caenorhabditis briggsae] 0.0
blast AAD33831 warthog protein WRT-6 [Caenorhabditis elegans] 1.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR001767 EMBL-EBI Peptidase C46, hedgehog protein, hint region 1.2E-100
Pfam IPR001767 EMBL-EBI Peptidase C46, hedgehog protein, hint region 8.2E-96
Pfam IPR001767 EMBL-EBI Peptidase C46, hedgehog protein, hint region 1.2E-100
Sequence
Target Sequence 
>C. ELEGANS:188037_AT
tggcatgcagtgtttttccggagatatggaggtagagacagaagacggaatcaagatgat
caaggatctcaagattggcgacaaggttctcagcatggacgaggcttttgtgacgtattc
gcccgttatcatgtttttacacaaacgggatgaagagatcgcggaattcaatctcataga
aacagccaatggtcactcaattaaactgaccgataatcatttgatctacgtgtctgactg
ccgtactagatccgacttaaaattggtcgccgcgaaagaagtcaaaatggatgattgcat
tcacgtcaccacagacagcaatgtcgtcatcaagaaaaaggtatccaaaatcagcaaggt
catcgaaactggaatctactctccacttacttccaccggagatattattgtcaaccgagt
tctggcttcttgtcattccaacttggcgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGCATGCAGTGTTTTTCCGGAGAT 460 545 1248 Antisense
GATTGGCGACAAGGTTCTCAGCATG 513 435 1320 Antisense
GAGGCTTTTGTGACGTATTCGCCCG 663 401 1348 Antisense
TATTCGCCCGTTATCATGTTTTTAC 153 663 1363 Antisense
ATTTGATCTACGTGTCTGACTGCCG 38 19 1466 Antisense
CTGCCGTACTAGATCCGACTTAAAA 493 235 1485 Antisense
TGGATGATTGCATTCACGTCACCAC 612 549 1535 Antisense
AACTGGAATCTACTCTCCACTTACT 480 141 1614 Antisense
TCCACTTACTTCCACCGGAGATATT 180 589 1629 Antisense
TATTGTCAACCGAGTTCTGGCTTCT 455 663 1653 Antisense
CTTCTTGTCATTCCAACTTGGCGTT 583 221 1673 Antisense
Sequence Source Affymetrix Proprietary Database