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[188035_at]

Description K07A12.6 /REP_DB=WormBase Gene ID /WP=CE11874 /GEN=hot-5 /TR=O17937 /GB=CAB03181.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 14      4grp_highest_8_8 > 40      4grp_high_8_8 > 42      6grp_highest_nonorm > 70      6grp_total > 04      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 41      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 214
1 2 3 4 range
A 311
(3)
P 		199.5
(-0.2)
P 		178
(-0.8)
P 		103.7
(-3)
P 		207
B 273.1
(3)
P 		234
(1.5)
P 		191
(-0.1)
P 		113.9
(-3)
P 		159
C 232.7
(2.5)
P 		245.4
(3)
P 		185.5
(0.6)
P 		97.3
(-3)
P 		148
avr 272.3
(3) 		226.3
(1.4) 		184.8
(-0.1) 		105
(-3) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 318
1 2 3 4 5 6 range
A 245.2
(1.5)
P 		236.2
(1.1)
P 		278.5
(3)
P 		274.6
(2.8)
P 		221.9
(0.4)
P 		146.5
(-3)
P 		132
B 360.6
(3)
P 		313.9
(1.9)
P 		156.9
(-1.8)
P 		205.7
(-0.7)
P 		273.1
(0.9)
P 		106.9
(-3)
P 		254
C 412.8
(3)
P 		325.7
(1.4)
P 		212.4
(-0.8)
P 		247.7
(-0.1)
P 		242.5
(-0.2)
P 		94.8
(-3)
P 		318
avr 339.5
(3) 		291.9
(1.7) 		215.9
(-0.3) 		242.7
(0.4) 		245.8
(0.5) 		116.1
(-3) 		223
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 113.2
(3)
P 		43.3
(-1.3)
P 		46.5
(-1.1)
P 		15.2
(-3)
P 		98
B 87.1
(3)
P 		57.8
(0.2)
P 		44.2
(-1.1)
P 		24.3
(-3)
P 		63
C 77
(3)
P 		70.5
(2.4)
P 		64.6
(1.8)
P 		16.4
(-3)
P 		61
avr 92.4
(3) 		57.2
(0.1) 		51.8
(-0.3) 		18.6
(-3) 		74

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 45.2
(-1.8)
P 		84
(3)
P 		60.7
(0.1)
P 		69.6
(1.2)
P 		67
(0.9)
P 		35.7
(-3)
P 		48
B 125.5
(3)
P 		104.5
(1.6)
P 		38.1
(-3)
P 		54.5
(-1.9)
P 		111
(2)
P 		39.9
(-2.9)
P 		87
C 153
(3)
P 		99.4
(0.3)
P 		54.1
(-1.9)
P 		49.8
(-2.1)
P 		78.5
(-0.7)
P 		31.7
(-3)
P 		121
avr 107.9
(3) 		96
(2) 		51
(-1.7) 		58
(-1.2) 		85.5
(1.1) 		35.8
(-3) 		72
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188035_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2227
Sequence Type Exemplar sequence
Representative Public ID K07A12.6 NCBI
Target Description K07A12.6 /REP_DB=WormBase Gene ID /WP=CE11874 /GEN=hot-5 /TR=O17937 /GB=CAB03181.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059948(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059948 NCBI Caenorhabditis elegans Homolog of Odr-2(Two) family member (hot-5) (hot-5) mRNA, complete cds. 11/11 None
GENEFINDER00000033560 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8690409:8695380:-1 11/11 None
K07A12.6 ENSEMBL cdna:known chromosome:CEL140:I:8691345:8695234:-1 gene:K07A12.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000033548 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8691275-8695146(-) 96.5 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hot-5
Ensembl K07A12.6
Entrez Gene 172671 Entrez gene
SwissProt O17937 EMBL-EBI
WormBase CE11874 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03181 Hypothetical protein K07A12.6 [Caenorhabditis elegans] ref|NP_492349.1| Homolog of Odr-2(Two) family member (hot-5) [Caenorhabditis elegans] gb|AAK01418.1| Ly-6-related protein HOT-5 [Caenorhabditis elegans] 1.0E-101
blast CAB03181 Hypothetical protein K07A12.6 [Caenorhabditis elegans] ref|NP_492349.1| Homolog of Odr-2(Two) family member (hot-5) [Caenorhabditis elegans] gb|AAK01418.1| Ly-6-related protein HOT-5 [Caenorhabditis elegans] 4.0E-95
blast CAE68895 Hypothetical protein CBG14866 [Caenorhabditis briggsae] 6.0E-87
blast CAE68895 Hypothetical protein CBG14866 [Caenorhabditis briggsae] 7.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR010558 EMBL-EBI Caenorhabditis elegans ly-6-related 1.4E-82
Pfam IPR010558 EMBL-EBI Caenorhabditis elegans ly-6-related 1.4E-82
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000033560 3 15-37,221-238,258-280
Sequence
Target Sequence 
>C. ELEGANS:188035_AT
atgaggcagctgccttcaatattactgattcttgtctattttattcgttctgtggaattg
ttaaaatgcttttcctgcgcttcattcgaatacagagtacttttcgataaagacacttcg
ttatcgagaaaagtcagagtccccaaattcgatagattatgtgatttggaagagatggtt
cgtggttatgcaccagttgaagcatgccattcaacatgtgtcactattttcgaaccacag
tattttggaggtttacaatccttgcaacgtccatttctatacatccgtggttgtgctgat
cacatattttcttcaatgaaagatcgcccaattgaagttgaatttcttcatcgttcacca
atttgtgttaaacttcaactatcacaaatctatccacaagttcaagcaaatgaaatagtt
caagtgtgttcgtgtgacaaagacggttgcaattttgactcaatcaatgattcgactact
tccaaatataattcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGAGGCAGCTGCCTTCAATATTAC 273 45 13 Antisense
TAAAATGCTTTTCCTGCGCTTCATT 111 641 74 Antisense
TTTTCCTGCGCTTCATTCGAATACA 336 677 82 Antisense
ACTATTTTCGAACCACAGTATTTTG 494 101 235 Antisense
TTTGGAGGTTTACAATCCTTGCAAC 334 665 256 Antisense
TTCATCGTTCACCAATTTGTGTTAA 491 687 359 Antisense
AACTTCAACTATCACAAATCTATCC 538 137 383 Antisense
AAATCTATCCACAAGTTCAAGCAAA 356 117 398 Antisense
ATAGTTCAAGTGTGTTCGTGTGACA 265 19 427 Antisense
GTTCGTGTGACAAAGACGGTTGCAA 673 439 440 Antisense
TCGACTACTTCCAAATATAATTCCA 648 601 484 Antisense
Sequence Source Affymetrix Proprietary Database