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[188034_s_at]

Description ZK377.2 /REP_DB=WormBase Gene ID /WP=CE25688 /GEN=sax-3 /TR=O01632 /GB=AAB52657.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 26      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 60      6grp_total > 25      4grp_total > 46      6grp_highest_sub > De      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 399
1 2 3 4 range
A 575.9
(3)
P 		399
(-0.8)
P 		299.2
(-3)
P 		311.3
(-2.7)
P 		277
B 621.9
(3)
P 		401.1
(-0.9)
P 		279.5
(-3)
P 		316.3
(-2.4)
P 		342
C 678.8
(3)
P 		376.1
(-2.1)
P 		350.5
(-2.5)
P 		323.5
(-3)
P 		355
avr 625.5
(3) 		392.1
(-1.4) 		309.7
(-3) 		317
(-2.9) 		316

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 325
1 2 3 4 5 6 range
A 436.3
(-0.3)
P 		566.2
(3)
P 		335.8
(-2.9)
P 		333.7
(-2.9)
P 		337.4
(-2.8)
P 		330.1
(-3)
P 		236
B 505.7
(3)
P 		485.7
(2.5)
P 		383.9
(-0.1)
P 		385
(-0.1)
P 		270.9
(-3)
P 		343
(-1.2)
P 		235
C 579.4
(3)
P 		446.1
(0.5)
P 		411.7
(-0.1)
P 		254
(-3)
P 		305.3
(-2.1)
P 		296.5
(-2.2)
P 		325
avr 507.1
(3) 		499.3
(2.8) 		377.1
(-0.8) 		324.2
(-2.4) 		304.5
(-3) 		323.2
(-2.4) 		203
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 179
1 2 3 4 range
A 209.7
(3)
P 		86.6
(-1.5)
P 		78.2
(-1.8)
P 		45.6
(-3)
P 		164
B 198.3
(3)
P 		99.1
(-1.5)
P 		64.8
(-3)
P 		67.4
(-2.9)
P 		134
C 224.7
(3)
P 		108.1
(-1.1)
P 		122.2
(-0.6)
P 		54.5
(-3)
P 		170
avr 210.9
(3) 		97.9
(-1.4) 		88.4
(-1.7) 		55.8
(-3) 		155

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 164
1 2 3 4 5 6 range
A 80.4
(-2.7)
P 		201.4
(3)
P 		73.1
(-3)
P 		84.6
(-2.5)
P 		101.8
(-1.7)
P 		80.4
(-2.7)
P 		128
B 176
(3)
P 		161.7
(2)
P 		93.2
(-3)
P 		102
(-2.4)
P 		110.1
(-1.8)
P 		127.9
(-0.5)
P 		83
C 214.7
(3)
P 		136.1
(0.1)
P 		104.8
(-1)
P 		51.1
(-3)
P 		98.8
(-1.3)
P 		99.3
(-1.2)
P 		164
avr 157
(2.4) 		166.4
(3) 		90.4
(-2.2) 		79.2
(-3) 		103.6
(-1.3) 		102.5
(-1.4) 		87
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188034_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18291
Sequence Type Exemplar sequence
Representative Public ID ZK377.2 NCBI
Target Description ZK377.2 /REP_DB=WormBase Gene ID /WP=CE25688 /GEN=sax-3 /TR=O01632 /GB=AAB52657.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029819(11), NM_171652(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029819 NCBI Caenorhabditis elegans Sensory AXon guidance family member (sax-3) (sax-3) mRNA, complete cds. 11/11 None
NM_171652 NCBI Caenorhabditis elegans Sensory AXon guidance family member (sax-3) (sax-3) mRNA, complete cds. 11/11 A 
SNAP00000036064 ENSEMBL cdna:SNAP chromosome:CEL140:X:3452483:3455366:-1 11/11 None
ZK377.2b ENSEMBL cdna:known chromosome:CEL140:X:3451932:3465829:-1 gene:ZK377.2 11/11 A 
ZK377.2a ENSEMBL cdna:known chromosome:CEL140:X:3452483:3465829:-1 gene:ZK377.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000036069 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3452483-3465830(-) 100.0 100.0
Public Domain and Genome References
Ensembl ZK377.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM69080 Sensory axon guidance protein 3, isoform b [Caenorhabditis elegans] ref|NP_001024990.1| Sensory AXon guidance family member (sax-3) [Caenorhabditis elegans] gb|AAC38848.1| SAX-3 [Caenorhabditis elegans] 0.0
blast AAB52657 Sensory axon guidance protein 3, isoform a [Caenorhabditis elegans] ref|NP_741748.2| Sensory AXon guidance family member (sax-3) [Caenorhabditis elegans] 0.0
blast CAE68436 Hypothetical protein CBG14221 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003961 EMBL-EBI Fibronectin, type III 5.1E-23
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.6E-7
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.6E-12
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.2E-7
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.9E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.6E-8
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.0E-8
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.6E-12
Pfam IPR003961 EMBL-EBI Fibronectin, type III 5.1E-23
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.6E-7
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.6E-12
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.6E-12
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024990.1 1 874-896
NP_741748.2 1 874-896
ZK377.2b 1 874-896
ZK377.2a 1 874-896
SNAP00000036064 1 438-460
Sequence
Target Sequence 
>C. ELEGANS:188034_S_AT
actccatcaccggactagcggaagtcaacggtcggatagtccacctcacacagatgtgag
ctatgttcagcttcactcatccgatggaactggtagtagtaaggaaagaactggggagcg
gagaacaccaccgaataagactctgatggactttattccgccaccaccttccaatccacc
accacctggagggcacgtttatgacacagcaactaggcgtcagttgaatcgtggaagtac
tccacgagaagacacctacgattcggtcagtgacggagcttttgctcgggttgatgtgaa
tgcaaggccaacgagtcggaatcggaatttgggaggaaggccgctgaaagggaaacgaga
cgacgatagtcagcggtcttcgttgatgatggacgatgatggtggatcttctgaagctga
cggggagaactctgaaggagacgttccgcgtggaggtgttagaaaagcagttcctcgaat
gggtatctctgcaagtacgctggctcatagttgttacgggacaaacggcactgctcaacg
attccggtcaattccacgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTCCATCACCGGACTAGCGGAAGT 384 97 3231 Antisense
GAGCTATGTTCAGCTTCACTCATCC 153 389 3288 Antisense
GACTCTGATGGACTTTATTCCGCCA 120 375 3369 Antisense
GAAGACACCTACGATTCGGTCAGTG 402 337 3478 Antisense
TCGGTCAGTGACGGAGCTTTTGCTC 234 607 3493 Antisense
GATAGTCAGCGGTCTTCGTTGATGA 449 425 3595 Antisense
GTTCCTCGAATGGGTATCTCTGCAA 506 441 3700 Antisense
TATCTCTGCAAGTACGCTGGCTCAT 625 657 3714 Antisense
GCTGGCTCATAGTTGTTACGGGACA 279 295 3729 Antisense
GACAAACGGCACTGCTCAACGATTC 708 365 3750 Antisense
CAACGATTCCGGTCAATTCCACGTA 8 187 3766 Antisense
Sequence Source Affymetrix Proprietary Database