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[188026_at]

Description ZC155.1 /REP_DB=WormBase Gene ID /WP=CE01431 /GEN=nex-1 /TR=Q27864 /GB=AAB52702.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 50      4grp_highest_8_8 > 32      4grp_high_8_8 > 51      6grp_highest_nonorm > 26      6grp_total > 51      4grp_total > 60      6grp_highest_sub > 03_04      6grp_cgc > 60      6grp_high1_sub > 03_04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2802
1 2 3 4 range
A 3979.4
(3)
P 		3412.4
(1.8)
P 		3803.1
(2.6)
P 		1210.3
(-3)
P 		2769
B 3903.5
(2.9)
P 		3682
(2.4)
P 		3935.5
(3)
P 		1177.6
(-3)
P 		2758
C 3795.8
(3)
P 		3659.4
(2.7)
P 		3400.9
(2.1)
P 		1183.1
(-3)
P 		2613
avr 3892.9
(3) 		3584.6
(2.3) 		3713.2
(2.6) 		1190.3
(-3) 		2703

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2695
1 2 3 4 5 6 range
A 6839.4
(3)
P 		5196.3
(-2.6)
P 		6712.7
(2.6)
P 		6497.3
(1.8)
P 		5823.2
(-0.5)
P 		5075
(-3)
P 		1764
B 6135.1
(1.1)
P 		5590.8
(-0.5)
P 		6779.3
(3)
P 		5972.6
(0.6)
P 		5798.1
(0.1)
P 		4768.4
(-3)
P 		2011
C 5925.6
(0.7)
P 		5411.8
(-0.5)
P 		6979.7
(3)
P 		6931.5
(2.9)
P 		6010.2
(0.8)
P 		4284.4
(-3)
P 		2695
avr 6300
(1.5) 		5399.6
(-1) 		6823.9
(3) 		6467.1
(2) 		5877.2
(0.3) 		4709.3
(-3) 		2115
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1272
1 2 3 4 range
A 1448.9
(3)
P 		740.7
(-0.3)
P 		994.2
(0.9)
P 		177.2
(-3)
P 		1272
B 1244.8
(3)
P 		909.4
(1)
P 		911.9
(1)
P 		250.9
(-3)
P 		994
C 1256.5
(3)
P 		1052.1
(1.8)
P 		1185.3
(2.6)
P 		199.5
(-3)
P 		1057
avr 1316.7
(3) 		900.7
(0.7) 		1030.5
(1.4) 		209.2
(-3) 		1108

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1120
1 2 3 4 5 6 range
A 1260.8
(-2.8)
P 		1848.1
(3)
P 		1461.9
(-0.8)
P 		1647.9
(1)
P 		1757.2
(2.1)
P 		1235.6
(-3)
P 		613
B 2134.7
(1.3)
P 		1860.9
(-0.8)
P 		1646.2
(-2.5)
P 		1581.9
(-3)
P 		2355.7
(3)
P 		1778.1
(-1.5)
P 		774
C 2196.4
(3)
P 		1651.3
(-1.1)
P 		1776.5
(-0.1)
P 		1393.9
(-3)
P 		1944.8
(1.1)
P 		1434.2
(-2.7)
P 		803
avr 1864
(1.3) 		1786.8
(0.4) 		1628.2
(-1.4) 		1541.2
(-2.3) 		2019.2
(3) 		1482.6
(-3) 		537
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188026_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7694
Sequence Type Exemplar sequence
Representative Public ID ZC155.1 NCBI
Target Description ZC155.1 /REP_DB=WormBase Gene ID /WP=CE01431 /GEN=nex-1 /TR=Q27864 /GB=AAB52702.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065708(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065708 NCBI Caenorhabditis elegans anNEXin family member (nex-1) (nex-1) mRNA, complete cds. 11/11 A 
SNAP00000027528 ENSEMBL cdna:SNAP chromosome:CEL140:III:5225581:5226602:1 11/11 None
GENEFINDER00000027536 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5225581:5226602:1 11/11 None
ZC155.1.3 ENSEMBL cdna:known chromosome:CEL140:III:5225578:5226733:1 gene:ZC155.1 11/11 None
ZC155.1.1 ENSEMBL cdna:known chromosome:CEL140:III:5225580:5226757:1 gene:ZC155.1 11/11 A 
ZC155.1.2 ENSEMBL cdna:known chromosome:CEL140:III:5225580:5226900:1 gene:ZC155.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5225579-5226601(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nex-1
Ensembl ZC155.1
Entrez Gene 175714 Entrez gene
SwissProt Q27864 EMBL-EBI
WormBase CE01431 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1586411_AT annexin A13 cfa
CANINE_2:CFA.3796.1.A1_S_AT annexin A13 cfa
CHICKEN:GGA.9643.1.S1_AT annexin A13 gga
HG-U95AV2:32320_AT annexin A13 hs
HG-U133_PLUS_2:208323_S_AT annexin A13 hs
HG-U133A:208323_S_AT annexin A13 hs
HG-FOCUS:208323_S_AT annexin A13 hs
HG-U133A_2:208323_S_AT annexin A13 hs
HUGENEFL:Z11502_AT annexin A13 hs
U133_X3P:G4757753_3P_A_AT annexin A13 hs
MG-U74BV2:163746_AT annexin A13 mm
MOE430A:1424959_AT annexin A13 mm
MOUSE430A_2:1424959_AT annexin A13 mm
MOUSE430_2:1424959_AT annexin A13 mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
50819 negative regulation of coagulation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
5544 calcium-dependent phospholipid binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52702 Annexin family protein 1 [Caenorhabditis elegans] ref|NP_498109.1| anNEXin family member (nex-1) [Caenorhabditis elegans] gb|AAA99775.1| NEX1 annexin 1.0E-169
blast CAE73660 Hypothetical protein CBG21168 [Caenorhabditis briggsae] 1.0E-159
Protein Domains
Database ID Description E-Value
Pfam IPR001464 EMBL-EBI Annexin 3.8E-17
Pfam IPR001464 EMBL-EBI Annexin 1.2E-32
Pfam IPR001464 EMBL-EBI Annexin 1.9E-13
Pfam IPR001464 EMBL-EBI Annexin 1.0E-30
Sequence
Target Sequence 
>C. ELEGANS:188026_AT
gaattccgtgatcttttggtcagcttggtcactggatccaaagacggatctcatgatacc
aacgacgctcaggccaaggatgatgctgttcgcctgttcgccgacggaaaagccaagctt
gccaagaaggacggaacacacttccttcacatcctcgccactcaaaatcaataccagctc
cgcaaggtcttcgcctacttccaggaactcgctggtggctctatcgagaaaagcatcgag
aaggagttttccggagatctccagaaatcctacctcaccattgtccgtgccgcttccgac
aagcagaagttcttcgctcagcagctccacgcttccatgaaaggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTCCGTGATCTTTTGGTCAGCT 134 323 445 Antisense
TTGGTCAGCTTGGTCACTGGATCCA 312 709 460 Antisense
GATCTCATGATACCAACGACGCTCA 593 419 491 Antisense
GGCCAAGGATGATGCTGTTCGCCTG 181 545 516 Antisense
TTCGCCTGTTCGCCGACGGAAAAGC 544 695 533 Antisense
TCCTCGCCACTCAAAATCAATACCA 429 593 596 Antisense
GCCTACTTCCAGGAACTCGCTGGTG 311 281 637 Antisense
GGAGATCTCCAGAAATCCTACCTCA 278 519 697 Antisense
GTGCCGCTTCCGACAAGCAGAAGTT 6 479 731 Antisense
AGCAGAAGTTCTTCGCTCAGCAGCT 274 77 746 Antisense
CAGCTCCACGCTTCCATGAAAGGAC 524 203 766 Antisense
Sequence Source Affymetrix Proprietary Database