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[188017_s_at]

Description F54C1.3 /REP_DB=WormBase Gene ID /WP=CE11046 /GEN=mes-3 /TR=P91324 /GB=AAK21393.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 56      4grp_high_8_8 > 13      6grp_total > 47      4grp_total > 03      6grp_cgc > 27      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 284
1 2 3 4 range
A 170.7
(-0.2)
P 		88
(-3)
A 		126.6
(-1.7)
A 		264.7
(3)
P 		177
B 133.8
(-3)
P 		167.3
(-1.4)
P 		154.8
(-2)
P 		257.4
(3)
P 		124
C 181.4
(-1.9)
P 		160.3
(-2.4)
P 		136.9
(-3)
P 		371.9
(3)
P 		235
avr 162
(-2.1) 		138.5
(-3) 		139.4
(-3) 		298
(3) 		160

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 167
1 2 3 4 5 6 range
A 139.7
(0)
P 		130.2
(-0.7)
P 		102.3
(-2.7)
P 		119.6
(-1.5)
P 		98.4
(-3)
A 		180.9
(3)
P 		83
B 204.5
(2.4)
P 		150.5
(-0.2)
P 		148.1
(-0.3)
P 		148.4
(-0.2)
P 		89.9
(-3)
P 		217.5
(3)
P 		128
C 205
(0.9)
P 		187.3
(0.2)
P 		109
(-3)
P 		120.2
(-2.5)
P 		113.3
(-2.8)
P 		256.7
(3)
P 		148
avr 183.1
(1.2) 		156
(-0.2) 		119.8
(-2) 		129.4
(-1.5) 		100.5
(-3) 		218.4
(3) 		118
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 62.1
(3)
P 		19.1
(-3)
A 		33.1
(-1)
A 		38.7
(-0.3)
P 		43
B 42.7
(-0.8)
P 		41.3
(-1.3)
P 		35.9
(-3)
P 		54.8
(3)
P 		19
C 60.1
(2.1)
P 		46.1
(-3)
P 		47.7
(-2.4)
P 		62.7
(3)
P 		17
avr 55
(3) 		35.5
(-3) 		38.9
(-2) 		52.1
(2.1) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 25.8
(-2.1)
P 		46.3
(3)
P 		22.3
(-3)
P 		30.3
(-1)
P 		29.7
(-1.2)
A 		44
(2.4)
P 		24
B 71.1
(1.7)
P 		50.1
(-1.1)
P 		36
(-3)
P 		39.3
(-2.6)
P 		36.5
(-2.9)
P 		81.1
(3)
P 		45
C 76
(2)
P 		57.1
(0.2)
P 		27.8
(-2.6)
P 		24.2
(-3)
P 		36.7
(-1.8)
P 		85.9
(3)
P 		62
avr 57.6
(1.2) 		51.2
(0.2) 		28.7
(-3) 		31.3
(-2.6) 		34.3
(-2.2) 		70.3
(3) 		42
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188017_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2443
Sequence Type Exemplar sequence
Representative Public ID F54C1.3 NCBI
Target Description F54C1.3 /REP_DB=WormBase Gene ID /WP=CE11046 /GEN=mes-3 /TR=P91324 /GB=AAK21393.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026321(11), NM_001026322(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026321 NCBI Caenorhabditis elegans Maternal Effect Sterile family member (mes-3) (mes-3) mRNA, complete cds. 11/11 None
NM_001026322 NCBI Caenorhabditis elegans Maternal Effect Sterile family member (mes-3) (mes-3) mRNA, complete cds. 9/11 None
SNAP00000015269 ENSEMBL cdna:SNAP chromosome:CEL140:I:4996845:5002351:1 11/11 None
GENEFINDER00000015277 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4996845:5002853:1 10/11 None
F54C1.3a ENSEMBL cdna:known chromosome:CEL140:I:4996842:5003161:1 gene:F54C1.3 11/11 A 
F54C1.3b ENSEMBL cdna:known chromosome:CEL140:I:4996845:5002351:1 gene:F54C1.3 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4996844-5002351(+) 100.0 100.0
Public Domain and Genome References
Ensembl F54C1.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK21393 Maternal effect sterile protein 3, isoform a [Caenorhabditis elegans] ref|NP_001021492.1| Maternal Effect Sterile family member (mes-3) [Caenorhabditis elegans] sp|Q10665|MES3_CAEEL Polycomb protein mes-3 (Maternal-effect sterile 3 protein) pir||G87767 protein mes-3 [imported] - Caenorhabditis elegans 0.0
blast AAB01719 MES-3 0.0
blast AAR30207 Maternal effect sterile protein 3, isoform b [Caenorhabditis elegans] ref|NP_001021493.1| Maternal Effect Sterile family member (mes-3) [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:188017_S_AT
gtgccccagctgaatggcgaagaaaatggaaaaatcgaaggtcttatggaagcatatccg
atgaataatgctcacatgtattcagatattgagttcctgagaaaagaaaaattgaatctt
ccgaagaatgctcaaaaatatggcgaaatgacaattcttgactatccactaacgaagacg
gaaacggacgaatataataaaaagatgtctgtgatgaatgtcaaagattttgcggttaaa
cccagaaaacctattccaacagtcggaaataccactttaagtttggatttggaaggatgt
cctatatcgacattttcagaaatattcttcccaagtggaaaaactgaactattcaatatt
ctatgcagacatttctactctgaagctggacaacgccctggatgcttctcagcacaatgg
aaatctagagttttgactggcttcatcaacaaataccatcagtacattttcgacatgaaa
ttgaattctttattcgagaaacaaataaatctcatcgctatggtcagccctgactggaaa
ttagaagattttgaattgcccatggttcgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCCCCAGCTGAATGGCGAAGAAA 318 479 1648 Antisense
AATGCTCACATGTATTCAGATATTG 609 165 1714 Antisense
GACAATTCTTGACTATCCACTAACG 201 365 1797 Antisense
CCAGAAAACCTATTCCAACAGTCGG 376 273 1889 Antisense
TATGCAGACATTTCTACTCTGAAGC 532 655 2009 Antisense
CCCTGGATGCTTCTCAGCACAATGG 567 259 2043 Antisense
GAGTTTTGACTGGCTTCATCAACAA 184 399 2075 Antisense
ATACCATCAGTACATTTTCGACATG 701 21 2100 Antisense
TAAATCTCATCGCTATGGTCAGCCC 199 641 2153 Antisense
GGTCAGCCCTGACTGGAAATTAGAA 539 503 2169 Antisense
GATTTTGAATTGCCCATGGTTCGAT 650 429 2194 Antisense
Sequence Source Affymetrix Proprietary Database