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[188016_at]

Description W03B1.4 /REP_DB=WormBase Gene ID /WP=CE25136 /GEN=srs-1 /TR=O45887 /GB=AAC24441.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 74      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 86      6grp_total > 64      4grp_total > 57      6grp_cgc > 56      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 540
1 2 3 4 range
A 805.2
(-2.7)
P 		1010
(0.7)
P 		1148.9
(3)
P 		785.9
(-3)
P 		363
B 907.5
(-1)
P 		1114
(1.3)
P 		1268.5
(3)
P 		728.2
(-3)
P 		540
C 825.1
(-2.6)
P 		1155.8
(3)
P 		1063.9
(1.4)
P 		804.3
(-3)
P 		352
avr 845.9
(-1.9) 		1093.3
(2) 		1160.4
(3) 		772.8
(-3) 		388

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 331
1 2 3 4 5 6 range
A 969.1
(1.3)
P 		911.2
(0)
P 		1046.7
(3)
P 		959.7
(1.1)
P 		773.6
(-3)
P 		960.2
(1.1)
P 		273
B 894.8
(-2.1)
P 		858.9
(-3)
P 		1085.6
(2.5)
P 		960.7
(-0.5)
P 		1039.5
(1.4)
P 		1104.8
(3)
P 		246
C 821.7
(-3)
P 		833.6
(-2.7)
P 		1079.1
(3)
P 		1022.3
(1.7)
P 		938.6
(-0.3)
P 		1033.6
(1.9)
P 		257
avr 895.2
(-2.2) 		867.9
(-3) 		1070.5
(3) 		980.9
(0.3) 		917.2
(-1.5) 		1032.9
(1.9) 		203
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 256
1 2 3 4 range
A 293.2
(2.8)
P 		219.3
(0.4)
P 		300.3
(3)
P 		115
(-3)
P 		185
B 289.4
(2.8)
P 		275.1
(2.2)
P 		293.9
(3)
P 		155.2
(-3)
P 		139
C 273.1
(0.5)
P 		332.3
(2)
P 		370.8
(3)
P 		135.6
(-3)
P 		235
avr 285.2
(1.8) 		275.6
(1.5) 		321.7
(3) 		135.3
(-3) 		186

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 244
1 2 3 4 5 6 range
A 178.6
(-3)
P 		324
(3)
P 		228
(-1)
P 		243.4
(-0.3)
P 		233.4
(-0.7)
P 		233.8
(-0.7)
P 		145
B 311.3
(-1)
P 		285.9
(-1.9)
P 		263.6
(-2.7)
P 		254.5
(-3)
P 		422.3
(3)
P 		412
(2.6)
P 		168
C 304.6
(1.2)
P 		254.4
(-0.9)
P 		274.7
(-0)
P 		205.6
(-3)
P 		303.7
(1.2)
P 		346
(3)
P 		140
avr 264.8
(-1.1) 		288.1
(0.3) 		255.4
(-1.7) 		234.5
(-3) 		319.8
(2.3) 		330.6
(3) 		96
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188016_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10964
Sequence Type Exemplar sequence
Representative Public ID W03B1.4 NCBI
Target Description W03B1.4 /REP_DB=WormBase Gene ID /WP=CE25136 /GEN=srs-1 /TR=O45887 /GB=AAC24441.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068148(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068148 NCBI Caenorhabditis elegans Seryl tRNA Synthetase family member (srs-1) (srs-1) mRNA, complete cds. 11/11 None
SNAP00000015138 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4358657:4359868:1 11/11 None
GENEFINDER00000015175 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4358329:4359868:1 11/11 None
W03B1.4 ENSEMBL cdna:known chromosome:CEL140:IV:4357629:4359925:1 gene:W03B1.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4357639-4359874(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srs-1
Ensembl W03B1.4
Entrez Gene 177200 Entrez gene
SwissProt O45887 EMBL-EBI
WormBase CE25136 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264350_AT seryl-tRNA synthetase, putative / serine--tRNA ligase, putative at
ATGENOME1:12486_AT seryl-tRNA synthetase, putative / serine--tRNA ligase, putative at
CANINE_2:CFAAFFX.9380.1.S1_S_AT similar to Seryl-tRNA synthetase, mitochondrial precursor (Serine--tRNA ligase) (SerRSmt) cfa
DROSOPHILA_2:1636797_AT Seryl-tRNA synthetase dm
DROSGENOME1:143948_AT Seryl-tRNA synthetase dm
HG-U133_PLUS_2:218702_AT seryl-tRNA synthetase 2 hs
HG-U133A_2:218702_AT seryl-tRNA synthetase 2 hs
HG-U133A:218702_AT seryl-tRNA synthetase 2 hs
U133_X3P:G8923420_3P_AT seryl-tRNA synthetase 2 hs
HG-U95D:75337_S_AT seryl-tRNA synthetase 2 hs
HG-U95E:90392_AT seryl-tRNA synthetase 2 hs
HG-U95E:90394_G_AT seryl-tRNA synthetase 2 hs
HG-U95E:90396_AT seryl-tRNA synthetase 2 hs
MU11KSUBA:AA289655_AT seryl-aminoacyl-tRNA synthetase 2 mm
MU11KSUBB:MSA.22192.0_S_AT seryl-aminoacyl-tRNA synthetase 2 mm
MU11KSUBB:MSA.20073.0_S_AT seryl-aminoacyl-tRNA synthetase 2 mm
MG-U74AV2:95630_AT seryl-aminoacyl-tRNA synthetase 2 mm
MG-U74AV2:161681_I_AT seryl-aminoacyl-tRNA synthetase 2 mm
MG-U74AV2:161339_F_AT seryl-aminoacyl-tRNA synthetase 2 mm
MOE430A:1448522_AT seryl-aminoacyl-tRNA synthetase 2 mm
MOUSE430A_2:1448522_AT seryl-aminoacyl-tRNA synthetase 2 mm
MOUSE430_2:1448522_AT seryl-aminoacyl-tRNA synthetase 2 mm
MU19KSUBA:TC15250_AT Seryl-aminoacyl-tRNA synthetase 2 (Sars2), mRNA mm
MU19KSUBC:TC38655_AT Seryl-aminoacyl-tRNA synthetase 2 (Sars2), mRNA mm
RG-U34C:RC_AI236915_AT seryl-tRNA synthetase 2 (predicted) rn
RAE230B:1383252_AT seryl-tRNA synthetase 2 (predicted) rn
RAT230_2:1383252_AT seryl-tRNA synthetase 2 (predicted) rn
RG-U34B:RC_AA850115_AT Seryl-tRNA synthetase 2 (predicted) rn
YEAST_2:1770696_AT Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6418 tRNA aminoacylation for protein translation inferred from electronic annotation QuickGO AmiGO
6434 seryl-tRNA aminoacylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4812 tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
4828 serine-tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC24441 Seryl trna synthetase protein 1 [Caenorhabditis elegans] ref|NP_500549.1| Seryl tRNA Synthetase family member (srs-1) [Caenorhabditis elegans] dbj|BAA74732.1| Seryl-tRNA synthetase [Caenorhabditis elegans] 0.0
blast CAE58518 Hypothetical protein CBG01669 [Caenorhabditis briggsae] 0.0
blast AAC24441 Seryl trna synthetase protein 1 [Caenorhabditis elegans] ref|NP_500549.1| Seryl tRNA Synthetase family member (srs-1) [Caenorhabditis elegans] dbj|BAA74732.1| Seryl-tRNA synthetase [Caenorhabditis elegans] 1.0E-153
blast CAE58518 Hypothetical protein CBG01669 [Caenorhabditis briggsae] 1.0E-144
blast AAH55610 Seryl-tRNA synthetase 2 [Danio rerio] ref|NP_957473.1| seryl-tRNA synthetase 2 [Danio rerio] 1.0E-64
Protein Domains
Database ID Description E-Value
Pfam IPR002314 EMBL-EBI tRNA synthetases, class-II (G, H, P and S) 1.1E-31
Pfam IPR002314 EMBL-EBI tRNA synthetases, class-II (G, H, P and S) 1.1E-31
Pfam IPR002314 EMBL-EBI tRNA synthetases, class-II (G, H, P and S) 1.1E-31
Sequence
Target Sequence 
>C. ELEGANS:188016_AT
ctgaaatgggaatcgccgcgtttttacggggacgaactttcaaccaagatcaacttccga
ttcgattggtctcactgagtcgatgcttccgaactgagatcagcaagtcggcgagcgagg
cgaagttgtacagagttcatgagttcagcaaggttgagatgttcgtggtgagcacgccgg
agcaaagtgcggcagagctggattatttggtggaggtgcagaagggaacattccaggcgt
tgggtgttcattgcagacaacttgaaatgccatccgaagagcttggagcctctgccgccc
ggaaattcgacattgaagcttggatgccggggcgaaaactctacggagaggtttcatcgg
ccagtaattgcacggatttccagtctcgacgcctcggaatcaagtataaaactgctgatg
gaacgacgaaatacgctcatacgtgtaatggaaccgctcttgcgtcgactagagctctca
tttctgtgctggaaacttttcagaatgacaaaaaaggactcggcgagcttccagaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGAAATGGGAATCGCCGCGTTTTT 184 225 734 Antisense
CCGATTCGATTGGTCTCACTGAGTC 119 265 790 Antisense
GAGTCGATGCTTCCGAACTGAGATC 105 401 810 Antisense
CAGGCGTTGGGTGTTCATTGCAGAC 258 207 967 Antisense
TGCCGCCCGGAAATTCGACATTGAA 682 581 1026 Antisense
TACGGAGAGGTTTCATCGGCCAGTA 313 647 1075 Antisense
AATTGCACGGATTTCCAGTCTCGAC 664 177 1099 Antisense
GTCTCGACGCCTCGGAATCAAGTAT 102 471 1116 Antisense
ACCGCTCTTGCGTCGACTAGAGCTC 12 91 1186 Antisense
TAGAGCTCTCATTTCTGTGCTGGAA 247 653 1203 Antisense
AAAGGACTCGGCGAGCTTCCAGAAC 363 119 1246 Antisense
Sequence Source Affymetrix Proprietary Database