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[188014_s_at]

Description F32B6.7 /REP_DB=WormBase Gene ID /WP=CE09862 /GEN=ssp-32 /TR=O45433 /GB=CAB03041.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 89      4grp_total > 27      6grp_cgc > 59      6grp_cgc_sub > Da      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 56.1
(-2.3)
A 		44.7
(-3)
A 		58.3
(-2.2)
A 		141.5
(3)
A 		97
B 117.8
(1.2)
A 		134.2
(3)
P 		88.9
(-1.9)
A 		79
(-3)
A 		55
C 55.6
(-2.3)
A 		118.4
(1.7)
A 		45.2
(-3)
A 		138
(3)
A 		93
avr 76.5
(-1.7) 		99.1
(0.8) 		64.1
(-3) 		119.5
(3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 175
1 2 3 4 5 6 range
A 51.4
(-3)
A 		86.5
(-1.5)
A 		113.4
(-0.3)
A 		65.9
(-2.4)
A 		135.6
(0.7)
A 		188.1
(3)
P 		137
B 93.1
(-1.1)
A 		71.2
(-2)
A 		49.6
(-3)
A 		56.3
(-2.7)
A 		124.3
(0.3)
P 		185.2
(3)
P 		136
C 32.4
(-2.2)
A 		97.9
(0.5)
A 		13.3
(-3)
A 		93.7
(0.3)
A 		140.2
(2.3)
P 		157.9
(3)
P 		145
avr 59
(-3) 		85.2
(-1.7) 		58.8
(-3) 		72
(-2.3) 		133.4
(0.8) 		177.1
(3) 		118
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 20.4
(2.8)
A 		9.7
(-3)
A 		15.3
(0.1)
A 		20.7
(3)
A 		11
B 37.6
(3)
A 		33.1
(1.7)
P 		20.6
(-1.9)
A 		16.8
(-3)
A 		21
C 18.4
(-2.1)
A 		34.1
(3)
A 		15.8
(-3)
A 		23.3
(-0.5)
A 		18
avr 25.5
(2.9) 		25.6
(3) 		17.2
(-3) 		20.3
(-0.8) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 9.5
(-3)
A 		30.8
(0.5)
A 		24.7
(-0.5)
A 		16.7
(-1.8)
A 		40.9
(2.2)
A 		45.8
(3)
P 		36
B 32.4
(-0.9)
A 		23.7
(-1.8)
A 		12
(-3)
A 		14.9
(-2.7)
A 		50.5
(1)
P 		69.1
(3)
P 		57
C 12
(-2)
A 		29.9
(0.2)
A 		3.4
(-3)
A 		18.8
(-1.1)
A 		45.4
(2.1)
P 		52.8
(3)
P 		49
avr 18
(-2.4) 		28.1
(-0.9) 		13.4
(-3) 		16.8
(-2.5) 		45.6
(1.5) 		55.9
(3) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188014_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8588
Sequence Type Exemplar sequence
Representative Public ID F32B6.7 NCBI
Target Description F32B6.7 /REP_DB=WormBase Gene ID /WP=CE09862 /GEN=ssp-32 /TR=O45433 /GB=CAB03041.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069381(11), NM_069338(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069381 NCBI Caenorhabditis elegans Sperm-Specific family, class P family member (ssp-32) (ssp-32) mRNA, complete cds. 11/11 None
NM_069338 NCBI Caenorhabditis elegans T13F2.12 (T13F2.12) mRNA, complete cds. 11/11 None
SNAP00000041534 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9895390:9896022:1 11/11 None
SNAP00000043471 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9765680:9766311:-1 11/11 None
T13F2.12 ENSEMBL cdna:known chromosome:CEL140:IV:9765680:9766311:-1 gene:T13F2.12 11/11 None
F32B6.7 ENSEMBL cdna:known chromosome:CEL140:IV:9895390:9896022:1 gene:F32B6.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000043634 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9895395-9896028(+) 100.0 100.0
chrIV:9765685-9766317(-) 98.77 98.77
Public Domain and Genome References
Gene Title

Gene Symbol ssp-32
Ensembl T13F2.12
F32B6.7
Entrez Gene 177811 Entrez gene
191963 Entrez gene
SwissProt O45433 EMBL-EBI
Q94054 EMBL-EBI
WormBase CE09862 Wormbase
CE13640 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03041 Hypothetical protein F32B6.7 [Caenorhabditis elegans] ref|NP_501782.1| Sperm-Specific family, class P family member (ssp-32) [Caenorhabditis elegans] 5.0E-55
blast CAB03363 Hypothetical protein T13F2.12 [Caenorhabditis elegans] ref|NP_501739.1| T13F2.12 [Caenorhabditis elegans] 6.0E-55
blast CAB03363 Hypothetical protein T13F2.12 [Caenorhabditis elegans] ref|NP_501739.1| T13F2.12 [Caenorhabditis elegans] 2.0E-54
blast CAB03041 Hypothetical protein F32B6.7 [Caenorhabditis elegans] ref|NP_501782.1| Sperm-Specific family, class P family member (ssp-32) [Caenorhabditis elegans] 2.0E-54
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 8.6E-40
Sequence
Target Sequence 
>C. ELEGANS:188014_S_AT
tgccacatttccagcgtcaggaggaagttccacgcacacaattacgtcagtgaacgaatc
tcgaatggcgtttaaggtgaagtcatcaaataatgagcactaccgagttcgtcccgtgta
tggatttgtcgaggcaaggggaaaaatgaaatttgagataattcgcctcgagggtcctgt
gaaagatgataaaattatgctacaatatgctgaagttccagctgatgagacagatgctca
ggcaccattcaaagctggtgctcaacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCCACATTTCCAGCGTCAGGAGGA 461 585 36 Antisense
GTCAGGAGGAAGTTCCACGCACACA 281 467 51 Antisense
GTTCCACGCACACAATTACGTCAGT 594 439 62 Antisense
ATTACGTCAGTGAACGAATCTCGAA 676 11 76 Antisense
GAATCTCGAATGGCGTTTAAGGTGA 277 329 91 Antisense
AATAATGAGCACTACCGAGTTCGTC 5 173 124 Antisense
CGAGTTCGTCCCGTGTATGGATTTG 231 251 139 Antisense
CCCGTGTATGGATTTGTCGAGGCAA 283 259 148 Antisense
TGAGATAATTCGCCTCGAGGGTCCT 563 567 189 Antisense
TTCGCCTCGAGGGTCCTGTGAAAGA 146 697 197 Antisense
ACCATTCAAAGCTGGTGCTCAACAA 611 85 279 Antisense
Sequence Source Affymetrix Proprietary Database