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[188006_s_at]

Description ZC395.3 /REP_DB=WormBase Gene ID /WP=CE15207 /GEN=toc-1 /TR=Q23281 /GB=AAG00040.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 50      4grp_highest_8_8 > 06      4grp_high_8_8 > 30      6grp_highest_nonorm > 74      6grp_total > 60      4grp_total > 25      6grp_highest_sub > 03_04      6grp_cgc > 94      6grp_high1_sub > 03_04      6grp_cgc_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 429
1 2 3 4 range
A 574.5
(-3)
P 		778
(0.4)
P 		734.4
(-0.3)
P 		933.3
(3)
P 		359
B 542.3
(-3)
P 		707.5
(-0.7)
P 		699.3
(-0.8)
P 		971.2
(3)
P 		429
C 543.3
(-3)
P 		711.9
(-0.2)
P 		754.6
(0.6)
P 		900.1
(3)
P 		357
avr 553.4
(-3) 		732.5
(-0.2) 		729.4
(-0.2) 		934.9
(3) 		382

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 331
1 2 3 4 5 6 range
A 683.9
(2)
P 		555.7
(-3)
P 		708.4
(3)
P 		647.3
(0.6)
P 		609.2
(-0.9)
P 		581.2
(-2)
P 		153
B 671.7
(-0.1)
P 		639.9
(-2.5)
P 		711.6
(3)
P 		709.8
(2.9)
P 		633.2
(-3)
P 		638.5
(-2.6)
P 		78
C 597.2
(-1.1)
P 		618.5
(-0.7)
P 		688.8
(0.6)
P 		823.1
(3)
P 		492.2
(-3)
P 		596.5
(-1.1)
P 		331
avr 650.9
(-0.1) 		604.7
(-1.9) 		702.9
(2) 		726.7
(3) 		578.2
(-3) 		605.4
(-1.9) 		149
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 209.2
(3)
P 		168.9
(-0.3)
P 		192
(1.6)
P 		136.6
(-3)
P 		73
B 172.9
(-1.5)
P 		174.7
(-1.3)
P 		162
(-3)
P 		207
(3)
P 		45
C 179.9
(-1.5)
P 		204.7
(-0.1)
P 		263
(3)
P 		151.8
(-3)
P 		111
avr 187.3
(0.3) 		182.8
(-0.4) 		205.7
(3) 		165.1
(-3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 126.1
(-3)
P 		197.6
(3)
P 		154.3
(-0.6)
P 		164.2
(0.2)
P 		183.8
(1.8)
P 		141.5
(-1.7)
P 		72
B 233.7
(1.3)
P 		213
(-0.1)
P 		172.8
(-3)
P 		188
(-1.9)
P 		257.3
(3)
P 		238.1
(1.6)
P 		85
C 221.4
(3)
P 		188.7
(-0.2)
P 		175.3
(-1.5)
P 		165.5
(-2.4)
P 		159.3
(-3)
P 		199.7
(0.9)
P 		62
avr 193.7
(1.8) 		199.8
(2.9) 		167.5
(-3) 		172.6
(-2.1) 		200.1
(3) 		193.1
(1.7) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188006_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7630
Sequence Type Exemplar sequence
Representative Public ID ZC395.3 NCBI
Target Description ZC395.3 /REP_DB=WormBase Gene ID /WP=CE15207 /GEN=toc-1 /TR=Q23281 /GB=AAG00040.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065726(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065726 NCBI Caenorhabditis elegans similar to Transporter Of divalent Cations family member (toc-1) (toc-1) mRNA, complete cds. 11/11 None
SNAP00000016752 ENSEMBL cdna:SNAP chromosome:CEL140:III:5275372:5277399:1 11/11 None
GENEFINDER00000016759 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5275372:5278000:1 11/11 None
ZC395.3.2 ENSEMBL cdna:known chromosome:CEL140:III:5275208:5277754:1 gene:ZC395.3 11/11 None
ZC395.3.3 ENSEMBL cdna:known chromosome:CEL140:III:5275245:5277754:1 gene:ZC395.3 11/11 None
ZC395.3.1 ENSEMBL cdna:known chromosome:CEL140:III:5275372:5277754:1 gene:ZC395.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5275370-5277398(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZC395.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG00040 Similar to transporter of divalent cations protein 1, isoform a [Caenorhabditis elegans] ref|NP_498127.1| similar to Transporter Of divalent Cations family member (toc-1) [Caenorhabditis elegans] 1.0E-174
blast AAZ32791 Similar to transporter of divalent cations protein 1, isoform b [Caenorhabditis elegans] 1.0E-174
Protein Domains
Database ID Description E-Value
Pfam IPR002524 EMBL-EBI Cation efflux protein 1.7E-43
Pfam IPR004916 EMBL-EBI Ubiquinone biosyntheis protein COQ7 1.9E-20
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498127.1 3 20-39,43-65,78-100
ZC395.3.2 3 20-39,43-65,78-100
ZC395.3.3 3 20-39,43-65,78-100
ZC395.3.1 3 20-39,43-65,78-100
SNAP00000016752 3 20-39,43-65,78-100
GENEFINDER00000016759 3 20-39,43-65,78-100
Sequence
Target Sequence 
>C. ELEGANS:188006_S_AT
gacttcgcatgggctgatccagtatgctgtctgctgctctctgttgctgtgttctcaacg
atgtatccactatcaacatatactggaatgattctacttcaaacaactcctccgcacttg
attaatcaaattgatcggtgtatttctgaagcttctcacatagacggtgttcttgagttg
aagagtggacgtttctggcaactggattttaactcgctggttggcactgttgatgttcgt
gtacgacgagatgcggatgagcagaatgttttggctcatgtaaccgaaaagttttcatct
gtaatcacagtgctgactgttcaagttgtcaaagacgctgcatggtctgcaggagagcaa
gttccatattcgaatggacatattcataagagtgaaggaaatcattcacatgataatggg
catggacattctcatgatcataatgatcatggacacagtcatggacatgatgatcatggc
cacgactctcatggacatagtcatgatcataacgaacatgatcatggtcacagtcatgga
ggaaataatgataatcatggacacagtcattctgcaggatcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTTCGCATGGGCTGATCCAGTAT 184 371 673 Antisense
TGATCCAGTATGCTGTCTGCTGCTC 41 567 687 Antisense
CTCTCTGTTGCTGTGTTCTCAACGA 218 231 709 Antisense
GTTCTCAACGATGTATCCACTATCA 328 443 723 Antisense
GATCGGTGTATTTCTGAAGCTTCTC 381 417 805 Antisense
GAAGCTTCTCACATAGACGGTGTTC 649 341 820 Antisense
AGAGTGGACGTTTCTGGCAACTGGA 616 63 854 Antisense
GGCAACTGGATTTTAACTCGCTGGT 261 535 869 Antisense
CTCGCTGGTTGGCACTGTTGATGTT 304 233 885 Antisense
TCATGGCCACGACTCTCATGGACAT 412 623 1146 Antisense
GGACACAGTCATTCTGCAGGATCAG 221 527 1231 Antisense
Sequence Source Affymetrix Proprietary Database