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[187999_at]

Description K08H10.1 /REP_DB=WormBase Gene ID /WP=CE18875 /GEN=lea-1 /TR=O16527 /GB=CAB05543.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 98      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 49      6grp_total > 97      4grp_total > 02      6grp_highest_sub > In      6grp_cgc > 69      6grp_high1_sub > In      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6328
1 2 3 4 range
A 574
(-3)
P 		792.8
(-2.8)
P 		755.6
(-2.8)
P 		6870
(3)
P 		6296
B 592.9
(-3)
P 		666.9
(-2.9)
P 		825.4
(-2.8)
P 		6421.6
(3)
P 		5829
C 632.2
(-2.9)
P 		542.3
(-3)
P 		811.2
(-2.7)
P 		5842.2
(3)
P 		5300
avr 599.7
(-3) 		667.3
(-2.9) 		797.4
(-2.8) 		6377.9
(3) 		5778

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8956
1 2 3 4 5 6 range
A 777.1
(-3)
P 		1241.5
(-2.7)
P 		1494.4
(-2.5)
P 		3011
(-1.5)
P 		6002.4
(0.5)
P 		9683.3
(3)
P 		8906
B 822.2
(-3)
P 		851.2
(-3)
P 		1639.3
(-2.4)
P 		3014.6
(-1.3)
P 		6059
(1.1)
P 		8473.3
(3)
P 		7651
C 1128.5
(-2.7)
P 		727.4
(-3)
P 		1950.6
(-2)
P 		2301.4
(-1.8)
P 		7765.4
(2.5)
P 		8403.6
(3)
P 		7676
avr 909.3
(-3) 		940
(-3) 		1694.8
(-2.4) 		2775.7
(-1.6) 		6608.9
(1.3) 		8853.4
(3) 		7944
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1213
1 2 3 4 range
A 209
(-2.7)
P 		172.1
(-3)
P 		197.5
(-2.8)
P 		1005.6
(3)
P 		834
B 189.1
(-2.9)
P 		164.7
(-3)
P 		191.3
(-2.9)
P 		1368.4
(3)
P 		1204
C 209.3
(-2.6)
P 		155.9
(-3)
P 		282.7
(-2.1)
P 		985.1
(3)
P 		829
avr 202.5
(-2.8) 		164.2
(-3) 		223.8
(-2.6) 		1119.7
(3) 		956

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3017
1 2 3 4 5 6 range
A 143.2
(-3)
P 		441.5
(-2.2)
P 		325.4
(-2.5)
P 		763.7
(-1.3)
P 		1811.3
(1.5)
P 		2357.6
(3)
P 		2214
B 286.1
(-3)
P 		283.3
(-3)
P 		398.1
(-2.8)
P 		798.5
(-1.9)
P 		2461.7
(1.5)
P 		3159.7
(3)
P 		2876
C 418.3
(-2.5)
P 		222
(-3)
P 		496.5
(-2.4)
P 		462.8
(-2.4)
P 		2512.8
(2.3)
P 		2813.2
(3)
P 		2591
avr 282.5
(-3) 		315.6
(-2.9) 		406.7
(-2.7) 		675
(-2.1) 		2261.9
(1.8) 		2776.8
(3) 		2494
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187999_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11612
Sequence Type Exemplar sequence
Representative Public ID K08H10.1 NCBI
Target Description K08H10.1 /REP_DB=WormBase Gene ID /WP=CE18875 /GEN=lea-1 /TR=O16527 /GB=CAB05543.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028871(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028871 NCBI Caenorhabditis elegans plant Late Embryo Abundant (LEA) related family member (lea-1) (lea-1) mRNA, complete cds. 11/11 None
SNAP00000005156 ENSEMBL cdna:SNAP chromosome:CEL140:V:10023145:10027646:1 11/11 None
GENEFINDER00000005158 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10021973:10028042:1 11/11 None
K08H10.1.1 ENSEMBL cdna:known chromosome:CEL140:V:10023125:10025818:1 gene:K08H10.1 11/11 None
K08H10.1.2 ENSEMBL cdna:known chromosome:CEL140:V:10023127:10025816:1 gene:K08H10.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_073174 1/11 Cross Hyb Matching Probes A 
K08H10.2a 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10023144-10025675(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol lea-1
Ensembl K08H10.1
Entrez Gene 3564838 Entrez gene
SwissProt O16527 EMBL-EBI
WormBase CE18875 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:265379_AT late embryogenesis abundant domain-containing protein / LEA domain-containing protein at
Protein Similarities
Method ID Description E-Value
blast CAB05543 Hypothetical protein K08H10.1 [Caenorhabditis elegans] ref|NP_001024042.1| plant Late Embryo Abundant (LEA) related family member (lea-1) [Caenorhabditis elegans] gb|AAB69446.1| Ce-LEA [Caenorhabditis elegans] 0.0
blast CAA98278 Hypothetical protein K08H10.2b [Caenorhabditis elegans] emb|CAI46598.1| Hypothetical protein K08H10.2b [Caenorhabditis elegans] ref|NP_001024044.1| K08H10.2b [Caenorhabditis elegans] 0.0
blast CAA98279 Hypothetical protein K08H10.2a [Caenorhabditis elegans] emb|CAB05548.2| Hypothetical protein K08H10.2a [Caenorhabditis elegans] ref|NP_505575.2| K08H10.2a [Caenorhabditis elegans] 0.0
blast CAE61064 Hypothetical protein CBG04813 [Caenorhabditis briggsae] 1.0E-180
Protein Domains
Database ID Description E-Value
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 5.8E-11
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 8.7E-19
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 5.1E-21
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 2.0E-18
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 3.4E-10
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 1.3E-15
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 3.0E-7
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 1.9E-20
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 2.7E-12
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 2.8E-22
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 3.2E-7
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 2.3E-14
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 1.6E-14
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 8.0E-9
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 2.0E-17
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 6.0E-9
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 9.6E-11
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 5.8E-11
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 8.7E-19
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 5.1E-21
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 2.0E-18
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 3.4E-10
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 1.3E-15
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 3.0E-7
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 1.9E-20
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 2.7E-12
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 2.8E-22
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 3.2E-7
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 2.3E-14
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 1.6E-14
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 8.0E-9
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 2.0E-17
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 6.0E-9
Pfam IPR004238 EMBL-EBI Late embryogenesis abundant protein 9.6E-11
Sequence
Target Sequence 
>C. ELEGANS:187999_AT
gatgcttgggactccacaaaggaccacgccgctgatgccaaggataaggcttccgatgcc
gcaggagacgccaaggacaagtccaagtctctaaccgaaaaggctggtgatgctatttct
ggagcttacgattctgtcaaagaaaaggcttccgacatcgcggactccttcaaggcccac
tcaaccaactccaaggataacgttgagaacaaagcgtctgacgcttataactctgccaag
gataaggcttccgacgcctgggacaaaaccaaggacaaggctggcgaggccaaggacaag
gctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGCTTGGGACTCCACAAAGGACC 565 407 1591 Antisense
CCACGCCGCTGATGCCAAGGATAAG 276 273 1614 Antisense
TGCCGCAGGAGACGCCAAGGACAAG 102 585 1647 Antisense
GGACAAGTCCAAGTCTCTAACCGAA 690 525 1665 Antisense
AAAGGCTGGTGATGCTATTTCTGGA 442 121 1689 Antisense
ATTTCTGGAGCTTACGATTCTGTCA 260 17 1705 Antisense
CCCACTCAACCAACTCCAAGGATAA 116 259 1766 Antisense
CGTCTGACGCTTATAACTCTGCCAA 121 247 1805 Antisense
CTGCCAAGGATAAGGCTTCCGACGC 243 237 1823 Antisense
TTCCGACGCCTGGGACAAAACCAAG 14 699 1839 Antisense
GCTGGCGAGGCCAAGGACAAGGCTG 298 293 1870 Antisense
Sequence Source Affymetrix Proprietary Database