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[187990_s_at]

Description Y48B6A.8 /REP_DB=WormBase Gene ID /WP=CE28131 /GEN=ace-3 /TR=Q9U295 /GB=CAB54453.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 07      4grp_total > 46      6grp_cgc > 12      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 292
1 2 3 4 range
A 309.5
(3)
P 		100.8
(-1.3)
A 		19
(-3)
A 		65.2
(-2)
A 		291
B 266.4
(3)
P 		100.4
(-1.9)
A 		99
(-2)
A 		63.8
(-3)
A 		203
C 257
(3)
P 		77.6
(-1.5)
A 		100.7
(-0.9)
A 		17.6
(-3)
A 		239
avr 277.6
(3) 		92.9
(-1.8) 		72.9
(-2.4) 		48.9
(-3) 		229

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 340
1 2 3 4 5 6 range
A 357.8
(3)
P 		176
(-0.3)
P 		39.2
(-2.7)
A 		45.8
(-2.6)
A 		23.1
(-3)
A 		77.1
(-2)
A 		335
B 311.1
(3)
P 		185
(0.4)
P 		19.6
(-3)
A 		129.7
(-0.7)
A 		91
(-1.5)
A 		41.1
(-2.6)
A 		292
C 360
(3)
P 		96.5
(-1.7)
A 		109.4
(-1.5)
A 		23.6
(-3)
A 		43.9
(-2.6)
A 		67.4
(-2.2)
A 		336
avr 343
(3) 		152.5
(-0.9) 		56.1
(-2.9) 		66.4
(-2.7) 		52.7
(-3) 		61.9
(-2.8) 		290
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 110
1 2 3 4 range
A 112.7
(3)
P 		21.9
(-2.1)
A 		5
(-3)
A 		9.5
(-2.7)
A 		108
B 85
(3)
P 		24.8
(-2.1)
A 		22.9
(-2.2)
A 		13.6
(-3)
A 		71
C 85.1
(3)
P 		22.3
(-1.6)
A 		35.1
(-0.7)
A 		3
(-3)
A 		82
avr 94.3
(3) 		23
(-2) 		21
(-2.1) 		8.7
(-3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 66
(3)
P 		62.6
(2.7)
P 		8.5
(-2.8)
A 		11.6
(-2.5)
A 		7
(-3)
A 		18.8
(-1.8)
A 		59
B 108.3
(3)
P 		61.6
(0.3)
P 		4.8
(-3)
A 		34.3
(-1.3)
A 		37
(-1.1)
A 		15.3
(-2.4)
A 		104
C 133.4
(3)
P 		29.4
(-1.8)
A 		27.8
(-1.9)
A 		4.7
(-3)
A 		14.2
(-2.6)
A 		22.6
(-2.2)
A 		129
avr 102.6
(3) 		51.2
(-0.5) 		13.7
(-3) 		16.9
(-2.8) 		19.4
(-2.6) 		18.9
(-2.6) 		89
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187990_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3788
Sequence Type Exemplar sequence
Representative Public ID Y48B6A.8 NCBI
Target Description Y48B6A.8 /REP_DB=WormBase Gene ID /WP=CE28131 /GEN=ace-3 /TR=Q9U295 /GB=CAB54453.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064562(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064562 NCBI Caenorhabditis elegans abnormal ACEtylcholinesterase family member (ace-3) (ace-3) mRNA, complete cds. 11/11 None
SNAP00000022964 ENSEMBL cdna:SNAP chromosome:CEL140:II:14205573:14210680:1 11/11 None
GENEFINDER00000022984 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14205573:14209776:1 11/11 None
Y48B6A.8 ENSEMBL cdna:known chromosome:CEL140:II:14205524:14210071:1 gene:Y48B6A.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14205569-14209023(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ace-3
Ensembl Y48B6A.8
Entrez Gene 175076 Entrez gene
SwissProt Q9U295 EMBL-EBI
WormBase CE28131 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6581 acetylcholine catabolism inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4104 cholinesterase activity inferred from electronic annotation QuickGO AmiGO
3990 acetylcholinesterase activity inferred from direct assay QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB54453 Hypothetical protein Y48B6A.8 [Caenorhabditis elegans] ref|NP_496963.1| abnormal ACEtylcholinesterase family member (ace-3) [Caenorhabditis elegans] 0.0
blast AAC14022 acetylcholinesterase [Caenorhabditis elegans] 0.0
blast AAF80377 class C acetylcholinesterase [Caenorhabditis briggsae] 0.0
blast CAE59452 Hypothetical protein CBG02828 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002018 EMBL-EBI Carboxylesterase, type B 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:187990_S_AT
gaatgtgtggacactgcgttcgcagttcagccggaattagtccgaacagctgccaagtat
gtgtactcggatccaaagtgtacggacccgaagaagaaaaccgatttctatacggaacag
gtgaaccagatggtcggtgactacttcttcacgtgtgacagtatatggtttgcgcacaac
tatccgaaaatggccgggaatcaatcgaatgtttttgtctactattttgatcagccatcc
agtgcaaatccctggccaaaatggacgggagtaatgcatggatatgaaatcgagtatgtt
ttcggagtacctcttcataacacaactgctggctacacaaaagaggagatggatgtttcc
gagaaagtcatcgatttctggaccacatttgccaatacaggtgtaccctctcttcggaag
cgagcagtaggaacaacacaaaagatcaaatgggatcggtacgacggaaccgatcacacg
ac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATGTGTGGACACTGCGTTCGCAG 212 333 1153 Antisense
GAATTAGTCCGAACAGCTGCCAAGT 449 325 1186 Antisense
AGATGGTCGGTGACTACTTCTTCAC 629 67 1280 Antisense
TATGGTTTGCGCACAACTATCCGAA 363 655 1316 Antisense
TGTCTACTATTTTGATCAGCCATCC 99 559 1368 Antisense
CAGCCATCCAGTGCAAATCCCTGGC 297 203 1384 Antisense
GAGTATGTTTTCGGAGTACCTCTTC 349 399 1444 Antisense
TTCATAACACAACTGCTGGCTACAC 165 689 1466 Antisense
ATCGATTTCTGGACCACATTTGCCA 559 35 1522 Antisense
TACCCTCTCTTCGGAAGCGAGCAGT 633 647 1556 Antisense
GGTACGACGGAACCGATCACACGAC 337 499 1610 Antisense
Sequence Source Affymetrix Proprietary Database