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[187983_at]

Description F09E8.2 /REP_DB=WormBase Gene ID /WP=CE05579 /GEN=msh-5 /TR=Q19267 /GB=CAA98055.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 44      4grp_highest_8_8 > 18      4grp_high_8_8 > 62      6grp_highest_nonorm > 71      6grp_total > 20      4grp_total > 56      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 321
1 2 3 4 range
A 335.6
(-3)
P 		436.3
(1)
P 		486.9
(3)
P 		345.7
(-2.6)
P 		151
B 344.1
(-0.4)
P 		450.5
(1.6)
P 		519.9
(3)
P 		212
(-3)
P 		308
C 395.2
(-1.4)
P 		423.4
(-0.5)
P 		532.7
(3)
P 		344.8
(-3)
P 		188
avr 358.3
(-1.4) 		436.7
(0.8) 		513.2
(3) 		300.8
(-3) 		212

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 204
1 2 3 4 5 6 range
A 290.5
(-1.3)
P 		417
(3)
P 		398.4
(2.4)
P 		354
(0.9)
P 		239.9
(-3)
P 		325.9
(-0.1)
P 		177
B 339.1
(-1.1)
P 		427.9
(3)
P 		316.2
(-2.1)
P 		375.4
(0.6)
P 		299.5
(-2.9)
P 		297.3
(-3)
P 		131
C 352
(0.2)
P 		443.4
(3)
P 		378.1
(1)
P 		339.4
(-0.2)
P 		245.7
(-3)
P 		370.4
(0.8)
P 		198
avr 327.2
(-0.7) 		429.4
(3) 		364.2
(0.7) 		356.3
(0.4) 		261.7
(-3) 		331.2
(-0.5) 		168
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 141
1 2 3 4 range
A 122.2
(2.6)
P 		94.7
(0.4)
P 		127.3
(3)
P 		50.6
(-3)
P 		77
B 109.7
(2.1)
P 		111.3
(2.3)
P 		120.5
(3)
P 		45.2
(-3)
P 		75
C 130.8
(0.4)
P 		121.7
(-0)
P 		185.7
(3)
P 		58.1
(-3)
P 		128
avr 120.9
(1.5) 		109.2
(0.7) 		144.5
(3) 		51.3
(-3) 		93

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 53.6
(-3)
P 		148.3
(3)
P 		86.8
(-0.9)
P 		89.8
(-0.7)
P 		72.4
(-1.8)
P 		79.3
(-1.4)
P 		95
B 118
(0.8)
P 		142.4
(3)
P 		76.8
(-3)
P 		99.4
(-0.9)
P 		121.7
(1.1)
P 		110.8
(0.1)
P 		66
C 130.5
(2.6)
P 		135.3
(3)
P 		96.2
(-0.5)
P 		68.3
(-3)
P 		79.5
(-2)
P 		124
(2)
P 		67
avr 100.7
(-1.4) 		142
(3) 		86.6
(-2.9) 		85.8
(-3) 		91.2
(-2.4) 		104.7
(-1) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187983_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8830
Sequence Type Exemplar sequence
Representative Public ID F09E8.2 NCBI
Target Description F09E8.2 /REP_DB=WormBase Gene ID /WP=CE05579 /GEN=msh-5 /TR=Q19267 /GB=CAA98055.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070129(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070129 NCBI Caenorhabditis elegans F09E8.2 (F09E8.2) mRNA, complete cds. 11/11 None
F09E8.2 ENSEMBL cdna:known chromosome:CEL140:IV:13150866:13154418:-1 gene:F09E8.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000017198 4/11 Cross Hyb Matching Probes None
GENEFINDER00000017208 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13150871-13154305(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F09E8.2
Entrez Gene 178267 Entrez gene
SwissProt Q19267 EMBL-EBI
WormBase CE05579 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1599655_S_AT similar to cysteine-rich with EGF-like domains 1 isoform 2 cfa
CANINE_2:CFA.20559.1.S1_S_AT similar to cysteine-rich with EGF-like domains 1 isoform 2 cfa
CANINE_2:CFA.2900.1.A1_AT similar to cysteine-rich with EGF-like domains 1 isoform 2 cfa
DROSGENOME1:145510_AT dm
DROSOPHILA_2:1633661_AT dm
HG-U95AV2:39115_AT cysteine-rich with EGF-like domains 1 hs
HU35KSUBA:RC_AA424398_AT cysteine-rich with EGF-like domains 1 hs
HG-U133A:203368_AT cysteine-rich with EGF-like domains 1 hs
HG-U133_PLUS_2:203368_AT cysteine-rich with EGF-like domains 1 hs
HG-U133A_2:203368_AT cysteine-rich with EGF-like domains 1 hs
U133_X3P:G7661643_3P_AT cysteine-rich with EGF-like domains 1 hs
MG-U74BV2:113172_AT cysteine-rich with EGF-like domains 1 mm
MOE430A:1416537_AT cysteine-rich with EGF-like domains 1 mm
MOUSE430A_2:1416537_AT cysteine-rich with EGF-like domains 1 mm
MOUSE430_2:1416537_AT cysteine-rich with EGF-like domains 1 mm
MU19KSUBC:TC37680_AT Cysteine-rich with EGF-like domains 1, mRNA (cDNA clone MGC:36401 IMAGE:5310111) mm
MU19KSUBC:TC40930_AT Cysteine-rich with EGF-like domains 1, mRNA (cDNA clone MGC:36401 IMAGE:5310111) mm
RG-U34C:RC_AI175044_AT cysteine-rich with EGF-like domains 1 (predicted) rn
RAE230A:1388594_AT cysteine-rich with EGF-like domains 1 (predicted) rn
RAT230_2:1388594_AT cysteine-rich with EGF-like domains 1 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98055 Hypothetical protein F09E8.2 [Caenorhabditis elegans] ref|NP_502530.1| F09E8.2 [Caenorhabditis elegans] 0.0
blast CAE58648 Hypothetical protein CBG01816 [Caenorhabditis briggsae] 1.0E-178
blast CAI20713 novel protein similar to vertebrate cysteine-rich with EGF-like domains 1 (CRELD1) (zgc:66383) [Danio rerio] 2.0E-56
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502530.1 2 300-317,322-341
F09E8.2 2 300-317,322-341
Sequence
Target Sequence 
>C. ELEGANS:187983_AT
gaaatctatgccgatactgtgatatcgagtatttcgaagaatctcggacagtgcagggtg
tcgtttgtaaaaaatgccacgagggctgccttggagtttgctcatcggagagctcaaaag
gatgctcaaaatgtaaaaatgggtggaaattgacagaagaagggtgcgcagacgtcaacg
aatgccaaaacgagtccgcatgcacaaaagagcacgaaatttgtgtgaacactgtcggat
cgttcaagtgtgaatgtaaggaaggatataagaaggatgatgagcaaaattgtcaatttg
acgtcgaagcatctcctgatcgcccgttcatgccaatcgaccagcaactcaaattgattg
ccttctcctctctcatcatcatcatcactttcgtcgtttggcacggatccccagtgctct
atgttttaacgggaatcaccatcgtcgccctgattcttgtcgatctttatgttaatcctg
acacaattccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAATCTATGCCGATACTGTGATAT 341 363 563 Antisense
GAAGAATCTCGGACAGTGCAGGGTG 644 339 598 Antisense
ATGCCACGAGGGCTGCCTTGGAGTT 152 41 636 Antisense
CTGCCTTGGAGTTTGCTCATCGGAG 59 237 648 Antisense
AATTTGACGTCGAAGCATCTCCTGA 226 173 857 Antisense
GTTCATGCCAATCGACCAGCAACTC 340 445 888 Antisense
ATCACTTTCGTCGTTTGGCACGGAT 118 27 946 Antisense
TCCCCAGTGCTCTATGTTTTAACGG 455 595 970 Antisense
TTAACGGGAATCACCATCGTCGCCC 606 637 988 Antisense
GCCCTGATTCTTGTCGATCTTTATG 120 287 1009 Antisense
TTATGTTAATCCTGACACAATTCCC 561 673 1029 Antisense
Sequence Source Affymetrix Proprietary Database