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[187980_at]

Description R12E2.3 /REP_DB=WormBase Gene ID /WP=CE18135 /GEN=rpn-8 /TR=O61792 /GB=AAC17024.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 83      4grp_highest_8_8 > 13      4grp_high_8_8 > 31      6grp_highest_nonorm > 57      6grp_total > 74      4grp_total > 24      6grp_cgc > 66      6grp_high1_sub > 04      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1497
1 2 3 4 range
A 1602.4
(-3)
P 		2341.1
(0.5)
P 		2478.1
(1.2)
P 		2862.2
(3)
P 		1260
B 1478.4
(-3)
P 		2108.3
(-0.1)
P 		2664.9
(2.5)
P 		2763.1
(3)
P 		1285
C 1620.3
(-3)
P 		2080
(-1)
P 		2536.8
(1.1)
P 		2975.3
(3)
P 		1355
avr 1567
(-3) 		2176.5
(-0.2) 		2559.9
(1.6) 		2866.9
(3) 		1300

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1701
1 2 3 4 5 6 range
A 1889.8
(-2.9)
P 		1867.6
(-3)
P 		2461.6
(-0.1)
P 		3100.5
(3)
P 		2161.8
(-1.6)
P 		2817
(1.6)
P 		1233
B 1697.8
(-1.8)
P 		1399.7
(-3)
P 		2099.3
(-0.1)
P 		2381.6
(1)
P 		2369.1
(1)
P 		2856.5
(3)
P 		1457
C 1734.2
(-3)
P 		1728
(-3)
P 		2686.7
(1.8)
P 		2915.2
(3)
P 		2160.5
(-0.8)
P 		2344.5
(0.1)
P 		1187
avr 1773.9
(-2.4) 		1665.1
(-3) 		2415.9
(1) 		2799.1
(3) 		2230.5
(-0) 		2672.7
(2.3) 		1134
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 465
1 2 3 4 range
A 583.4
(1.3)
P 		508.2
(-0.7)
P 		647.8
(3)
P 		419
(-3)
P 		229
B 471.4
(-3)
P 		520.7
(-1)
P 		617.5
(3)
P 		588.8
(1.8)
P 		146
C 536.4
(-2.5)
P 		598
(-1.5)
P 		884.2
(3)
P 		501.7
(-3)
P 		383
avr 530.4
(-2.2) 		542.3
(-1.9) 		716.5
(3) 		503.2
(-3) 		213

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 717
1 2 3 4 5 6 range
A 348.4
(-3)
P 		664.2
(1.3)
P 		536.1
(-0.4)
P 		786.3
(3)
P 		652.3
(1.2)
P 		685.9
(1.6)
P 		438
B 590.8
(-1.7)
P 		465.9
(-3)
P 		509.8
(-2.6)
P 		630.8
(-1.3)
P 		962.5
(2)
P 		1065.2
(3)
P 		599
C 642.8
(-0.3)
P 		527.3
(-3)
P 		683.8
(0.6)
P 		586.2
(-1.6)
P 		699.1
(1)
P 		784.8
(3)
P 		258
avr 527.3
(-3) 		552.5
(-2.5) 		576.6
(-2.1) 		667.8
(-0.3) 		771.3
(1.6) 		845.3
(3) 		318
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187980_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2305
Sequence Type Exemplar sequence
Representative Public ID R12E2.3 NCBI
Target Description R12E2.3 /REP_DB=WormBase Gene ID /WP=CE18135 /GEN=rpn-8 /TR=O61792 /GB=AAC17024.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058918(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058918 NCBI Caenorhabditis elegans proteasome Regulatory Particle, Non-ATPase-like family member (rpn-8) (rpn-8) mRNA, complete cds. 11/11 None
SNAP00000012684 ENSEMBL cdna:SNAP chromosome:CEL140:I:4175324:4177131:1 11/11 None
GENEFINDER00000012696 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4175324:4177131:1 11/11 None
R12E2.3.1 ENSEMBL cdna:known chromosome:CEL140:I:4175318:4177252:1 gene:R12E2.3 11/11 None
R12E2.3.2 ENSEMBL cdna:known chromosome:CEL140:I:4175320:4177252:1 gene:R12E2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4175323-4177131(+) 91.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rpn-8
Ensembl R12E2.3
Entrez Gene 172009 Entrez gene
SwissProt O61792 EMBL-EBI
WormBase CE18135 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250749_AT 26S proteasome non-ATPase regulatory subunit 7, putative / 26S proteasome regulatory subunit S12, putative / MOV34 protein, putative at
CANINE:1584170_AT similar to 26S proteasome non-ATPase regulatory subunit 7 (26S proteasome regulatory subunit rpn8) (26S proteasome regulatory subunit S12) (Proteasome subunit p40) (Mov34 protein) cfa
CANINE_2:CFA.20079.1.S1_S_AT similar to 26S proteasome non-ATPase regulatory subunit 7 (26S proteasome regulatory subunit rpn8) (26S proteasome regulatory subunit S12) (Proteasome subunit p40) (Mov34 protein) cfa
CANINE_2:CFA.9159.1.A1_AT similar to 26S proteasome non-ATPase regulatory subunit 7 (26S proteasome regulatory subunit rpn8) (26S proteasome regulatory subunit S12) (Proteasome subunit p40) (Mov34 protein) cfa
DROSOPHILA_2:1635259_AT Mov34 dm
DROSGENOME1:143270_AT Mov34 dm
CHICKEN:GGAAFFX.11938.1.S1_S_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) gga
HG-U95AV2:945_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HC-G110:945_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HG-U133_PLUS_2:201705_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HG-U133A:201705_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HG-FOCUS:201705_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HG-U133A_2:201705_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HU35KSUBA:D50063_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HUGENEFL:D50063_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
U133_X3P:G4506230_3P_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HG-U95AV2:40276_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HG-U133_PLUS_2:238738_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HG-U133B:238738_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HG-U133_PLUS_2:244515_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HG-U133B:244515_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HG-U95C:63133_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HG-U95D:83443_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HG-U95E:86457_F_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HG-U95E:86459_R_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
HU35KSUBC:RC_AA160805_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
U133_X3P:HS.177337.0.A1_3P_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
U133_X3P:HS.199832.0.A1_3P_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) hs
MU11KSUBA:M64640_S_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 mm
MG-U74AV2:103350_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 mm
MOE430A:1451056_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 mm
MOUSE430A_2:1451056_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 mm
MOUSE430_2:1451056_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 mm
MOE430A:1432820_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 mm
MOUSE430A_2:1432820_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 mm
MOUSE430_2:1432820_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 mm
MOE430A:1454368_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 mm
MOUSE430A_2:1454368_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 mm
MOUSE430_2:1454368_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 mm
RAE230A:1389245_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (predicted) rn
RAT230_2:1389245_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (predicted) rn
RAE230B:1379322_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (predicted) rn
RAT230_2:1379322_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (predicted) rn
RG-U34C:RC_AI070459_AT Proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (predicted) rn
YEAST_2:1774707_AT Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Sc
Protein Similarities
Method ID Description E-Value
blast NP_491319 proteasome Regulatory Particle, Non-ATPase-like family member (rpn-8) [Caenorhabditis elegans] gb|AAC17024.1| Proteasome regulatory particle, non-atpase-like protein 8 [Caenorhabditis elegans] 0.0
blast CAE66740 Hypothetical protein CBG12090 [Caenorhabditis briggsae] 1.0E-177
Protein Domains
Database ID Description E-Value
Pfam IPR000555 EMBL-EBI Peptidase M67, Mov34 1.5E-46
Sequence
Target Sequence 
>C. ELEGANS:187980_AT
gaactccaccaatcaaaaccttcgagcatgtgccttcagatatcggagccgaagaagctg
aggaagttggagtcgagcatcttttgagagatatcaaggaccaaactgctggaactcttt
cacagcgtatcaccgatcaacttatgggcttgagaggactgcaatcacagcttgaatcca
ttgagaagtatcttcatgatattgtgcgcggaactcttccagtcaaccatcatgtcattt
actatgttcaagaagttctcaatctcctgcctgacgtcactcatccagactacattgttt
cacagaatgtgcaaactaatgatcagctcatgtgtgtgtatatgggatctcttgtacgat
cggtggtcgctttgcacaacttaattgataacaagatctctctccagaaggctga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACTCCACCAATCAAAACCTTCGA 24 345 554 Antisense
AAAACCTTCGAGCATGTGCCTTCAG 557 131 568 Antisense
ATGTGCCTTCAGATATCGGAGCCGA 285 49 581 Antisense
ACCAAACTGCTGGAACTCTTTCACA 246 85 653 Antisense
TCTTTCACAGCGTATCACCGATCAA 1 617 669 Antisense
GGACTGCAATCACAGCTTGAATCCA 473 523 709 Antisense
TGATATTGTGCGCGGAACTCTTCCA 394 563 750 Antisense
TCAAGAAGTTCTCAATCTCCTGCCT 7 633 801 Antisense
TGACGTCACTCATCCAGACTACATT 701 569 825 Antisense
TTGTACGATCGGTGGTCGCTTTGCA 161 693 905 Antisense
ACAAGATCTCTCTCCAGAAGGCTGA 587 113 944 Antisense
Sequence Source Affymetrix Proprietary Database