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[187977_at]

Description T24D8.4 /REP_DB=WormBase Gene ID /WP=CE05008 /GEN=nlp-2 /TR=Q22738 /GB=AAA81445.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 21      4grp_total > 41      6grp_cgc > 63      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 11
(-3)
A 		17.6
(1.6)
A 		19.7
(3)
A 		14.3
(-0.7)
A 		9
B 7.2
(-3)
A 		20
(3)
A 		8
(-2.6)
A 		8.1
(-2.6)
A 		13
C 7.3
(-3)
A 		31.2
(1.9)
A 		36.7
(3)
A 		23.2
(0.2)
A 		29
avr 8.5
(-3) 		22.9
(3) 		21.5
(2.4) 		15.2
(-0.2) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 21.7
(-0.8)
A 		33.2
(1.5)
A 		21.8
(-0.8)
A 		10.7
(-3)
A 		25.4
(-0.1)
A 		40.6
(3)
A 		30
B 11.7
(-1.7)
A 		36.3
(3)
M 		18.5
(-0.4)
A 		5.6
(-2.9)
A 		19.9
(-0.1)
A 		4.9
(-3)
A 		31
C 34.1
(0.9)
A 		37.3
(1.2)
A 		49.4
(2.7)
A 		52.2
(3)
A 		28.1
(0.1)
A 		1.6
(-3)
A 		51
avr 22.5
(-0.9) 		35.6
(3) 		29.9
(1.3) 		22.8
(-0.9) 		24.5
(-0.3) 		15.7
(-3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 4
(0.8)
A 		3.8
(0.4)
A 		5.1
(3)
A 		2.1
(-3)
A 		3
B 2.3
(-1.9)
A 		4.9
(3)
A 		1.9
(-2.6)
A 		1.7
(-3)
A 		3
C 2.4
(-3)
A 		9
(0.8)
A 		12.8
(3)
A 		3.9
(-2.1)
A 		10
avr 2.9
(-2.6) 		5.9
(2) 		6.6
(3) 		2.6
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 4
(-2.1)
A 		11.8
(3)
A 		4.7
(-1.7)
A 		2.7
(-3)
A 		7.7
(0.3)
A 		9.9
(1.7)
A 		9
B 4.1
(-1.5)
A 		12.1
(3)
M 		4.5
(-1.3)
A 		1.5
(-3)
A 		8.1
(0.7)
A 		1.8
(-2.8)
A 		11
C 12.6
(3)
A 		11.4
(2.4)
A 		12.6
(3)
A 		10.5
(2)
A 		9.1
(1.3)
A 		0.5
(-3)
A 		12
avr 6.9
(-0.8) 		11.8
(3) 		7.3
(-0.5) 		4.9
(-2.4) 		8.3
(0.3) 		4.1
(-3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187977_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18224
Sequence Type Exemplar sequence
Representative Public ID T24D8.4 NCBI
Target Description T24D8.4 /REP_DB=WormBase Gene ID /WP=CE05008 /GEN=nlp-2 /TR=Q22738 /GB=AAA81445.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076024(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076024 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-23) (nlp-23) mRNA, complete cds. 11/11 None
SNAP00000017734 ENSEMBL cdna:SNAP chromosome:CEL140:X:2342451:2342931:-1 11/11 None
GENEFINDER00000017743 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2342451:2342931:-1 11/11 None
T24D8.4 ENSEMBL cdna:known chromosome:CEL140:X:2342451:2342931:-1 gene:T24D8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2342450-2342931(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-23
Ensembl T24D8.4
Entrez Gene 188856 Entrez gene
SwissProt Q22738 EMBL-EBI
WormBase CE05008 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81445 Neuropeptide-like protein protein 23 [Caenorhabditis elegans] ref|NP_508425.1| Neuropeptide-Like Protein family member (nlp-23) [Caenorhabditis elegans] 2.0E-63
blast CAE58891 Hypothetical protein CBG02133 [Caenorhabditis briggsae] 3.0E-11
Sequence
Target Sequence 
>C. ELEGANS:187977_AT
ctcacctcgtcatttggatggcacttcttggagtctcagctcatgctttggagctgggtt
tttactcggaacatccaacgtctaaagatgattcctacgaatcccaacttttagattcta
atgacctagaccaagatgatgctgtcgacttggcgacccctatgattgttatcccaaacg
acgaagatgagatctatgccgatgaagaaccggagccactaactatggagaagcgtcttt
atatcagtcgtcaaggattccggccagcaaagcgatcaatggcaattggcagagctggaa
tgcgcccagggaagcgtgcatttgcagctggatggaaccgcggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCACCTCGTCATTTGGATGGCACT 283 227 20 Antisense
TGGATGGCACTTCTTGGAGTCTCAG 252 549 34 Antisense
TGGAGTCTCAGCTCATGCTTTGGAG 410 547 48 Antisense
GCTGGGTTTTTACTCGGAACATCCA 187 293 72 Antisense
GATTCCTACGAATCCCAACTTTTAG 290 393 109 Antisense
GATGATGCTGTCGACTTGGCGACCC 481 411 154 Antisense
GACCCCTATGATTGTTATCCCAAAC 708 381 174 Antisense
GAAGCGTCTTTATATCAGTCGTCAA 513 343 249 Antisense
AATTGGCAGAGCTGGAATGCGCCCA 205 175 303 Antisense
GCCCAGGGAAGCGTGCATTTGCAGC 345 287 323 Antisense
TTTGCAGCTGGATGGAACCGCGGAA 372 667 340 Antisense
Sequence Source Affymetrix Proprietary Database