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[187974_s_at]

Description ZK381.4 /REP_DB=WormBase Gene ID /WP=CE25689 /GEN=pgl-1 /TR=Q9TZQ3 /GB=AAG00057.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 98      4grp_highest_8_8 > 12      4grp_high_8_8 > 39      6grp_total > 96      4grp_total > 40      6grp_cgc > 68      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 324
1 2 3 4 range
A 109
(-3)
P 		323.9
(2.3)
P 		351.2
(3)
P 		339.2
(2.7)
P 		242
B 168.8
(-3)
P 		303.5
(0.7)
P 		388.9
(3)
P 		292.2
(0.4)
P 		220
C 156.4
(-3)
P 		300.2
(0.1)
P 		433.1
(3)
P 		245.6
(-1.1)
P 		277
avr 144.7
(-3) 		309.2
(1) 		391.1
(3) 		292.3
(0.6) 		246

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1553
1 2 3 4 5 6 range
A 95.5
(-3)
A 		123.8
(-2.9)
P 		208.6
(-2.6)
P 		546.4
(-1.3)
P 		1137.1
(1)
P 		1648.8
(3)
P 		1553
B 100.6
(-3)
P 		160.8
(-2.7)
P 		257.4
(-2.2)
P 		516.3
(-0.9)
P 		962.8
(1.3)
P 		1300.9
(3)
P 		1200
C 109.6
(-3)
P 		165.1
(-2.7)
P 		331.8
(-1.9)
P 		492.9
(-1.2)
P 		1147.2
(2)
P 		1366.6
(3)
P 		1257
avr 101.9
(-3) 		149.9
(-2.8) 		265.9
(-2.3) 		518.5
(-1.1) 		1082.4
(1.4) 		1438.8
(3) 		1337
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 39.7
(-3)
P 		70.3
(0.5)
P 		91.8
(3)
P 		49.7
(-1.8)
P 		52
B 53.8
(-3)
P 		75
(0.5)
P 		90.1
(3)
P 		62.3
(-1.6)
P 		36
C 51.8
(-2.4)
P 		86.3
(-0.5)
P 		150.9
(3)
P 		41.4
(-3)
P 		110
avr 48.4
(-3) 		77.2
(-0.2) 		110.9
(3) 		51.1
(-2.7) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 468
1 2 3 4 5 6 range
A 17.6
(-3)
A 		44
(-2.6)
P 		45.4
(-2.6)
P 		138.6
(-1.1)
P 		343.1
(2.1)
P 		401.4
(3)
P 		384
B 35
(-3)
P 		53.5
(-2.8)
P 		62.5
(-2.6)
P 		136.8
(-1.6)
P 		391.2
(1.7)
P 		485.1
(3)
P 		450
C 40.6
(-3)
P 		50.4
(-2.9)
P 		84.5
(-2.4)
P 		99.1
(-2.2)
P 		371.2
(1.8)
P 		457.5
(3)
P 		417
avr 31.1
(-3) 		49.3
(-2.7) 		64.1
(-2.5) 		124.8
(-1.7) 		368.5
(1.9) 		448
(3) 		417
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187974_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9019
Sequence Type Exemplar sequence
Representative Public ID ZK381.4 NCBI
Target Description ZK381.4 /REP_DB=WormBase Gene ID /WP=CE25689 /GEN=pgl-1 /TR=Q9TZQ3 /GB=AAG00057.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068676(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068676 NCBI Caenorhabditis elegans P GranuLe abnormality family member (pgl-1) (pgl-1) mRNA, complete cds. 11/11 None
SNAP00000022642 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6974601:6980572:-1 11/11 None
GENEFINDER00000022648 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6974601:6977112:-1 11/11 None
ZK381.4.1 ENSEMBL cdna:known chromosome:CEL140:IV:6974426:6977127:-1 gene:ZK381.4 11/11 None
ZK381.4.3 ENSEMBL cdna:known chromosome:CEL140:IV:6974436:6977129:-1 gene:ZK381.4 11/11 None
ZK381.4.2 ENSEMBL cdna:known chromosome:CEL140:IV:6974436:6977233:-1 gene:ZK381.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6974606-6977118(-) 92.48 100.0
Public Domain and Genome References
Gene Title
Gene Symbol pgl-1
Ensembl ZK381.4
Entrez Gene 177461 Entrez gene
SwissProt Q9TZQ3 EMBL-EBI
WormBase CE25689 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
43186 P granule inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAG00057 P granule abnormality protein 1 [Caenorhabditis elegans] ref|NP_501077.1| P GranuLe abnormality family member (pgl-1) [Caenorhabditis elegans] sp|Q9TZQ3|PGL1_CAEEL P granule abnormality protein 1 gb|AAC36100.1| PGL-1 [Caenorhabditis elegans] 0.0
blast T29177 hypothetical protein ZK381.4 - Caenorhabditis elegans 0.0
blast AAC17755 Hypothetical protein C18G1.4a [Caenorhabditis elegans] ref|NP_504278.1| C18G1.4a [Caenorhabditis elegans] dbj|BAC87886.1| PGL-3 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001781 EMBL-EBI Zn-binding protein, LIM 1.3E-8
Sequence
Target Sequence 
>C. ELEGANS:187974_S_AT
ggattcttcgactttgctaagctcatcaagaaacattggagaaggtgttactgtaaaagc
ggtcgatcctgtcccagaaaaggttaacgatgcacaacaacagcaaacagttaacgagat
cgagatggcttcggatgccaatcaagatacttcttcaagtgcttcgccagaagtagcgcc
aagtttttccaccgatggctgggattctccaacgaaatcagtagctctgccaccgggcat
gcagcagatagatgaagaggaaaccacagttgctgacaaagattctacaccacaaccaca
agctcgtgctgaaactgcatgggggtcaggcgacgctactccaatgcctcttcctgcccc
gactaatcaatacaaagtcagtggatttggggaagccaaagttgcaaaaggattcggtca
atttgctccca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATTCTTCGACTTTGCTAAGCTCA 611 507 1581 Antisense
CTGTAAAAGCGGTCGATCCTGTCCC 443 225 1631 Antisense
TACTTCTTCAAGTGCTTCGCCAGAA 602 645 1728 Antisense
CCAAGTTTTTCCACCGATGGCTGGG 325 273 1759 Antisense
GATGGCTGGGATTCTCCAACGAAAT 122 407 1774 Antisense
AACGAAATCAGTAGCTCTGCCACCG 135 145 1791 Antisense
TGCCACCGGGCATGCAGCAGATAGA 366 587 1808 Antisense
GACAAAGATTCTACACCACAACCAC 339 365 1855 Antisense
AAACTGCATGGGGGTCAGGCGACGC 619 125 1892 Antisense
TCCTGCCCCGACTAATCAATACAAA 698 593 1932 Antisense
AAAGGATTCGGTCAATTTGCTCCCA 486 119 1987 Antisense
Sequence Source Affymetrix Proprietary Database