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[187973_s_at]

Description F22E12.4 /REP_DB=WormBase Gene ID /WP=CE27755 /GEN=egl-9 /TR=O45918 /GB=CAA16314.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 92      4grp_highest_8_8 > 63      4grp_high_8_8 > 28      6grp_highest_nonorm > 64      6grp_total > 94      4grp_total > 35      6grp_highest_sub > 04_05      6grp_cgc > 87      6grp_high1_sub > 04_05      6grp_cgc_sub > 05      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 500
1 2 3 4 range
A 859.7
(-2.1)
P 		1222.4
(3)
P 		793.9
(-3)
P 		1075.5
(0.9)
P 		429
B 969.4
(-1.2)
P 		1110.6
(0.7)
P 		826
(-3)
P 		1293.4
(3)
P 		467
C 948.6
(-1.3)
P 		1210.3
(3)
P 		842.8
(-3)
P 		930
(-1.6)
P 		368
avr 925.9
(-1.3) 		1181.1
(3) 		820.9
(-3) 		1099.6
(1.6) 		360

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 629
1 2 3 4 5 6 range
A 632.3
(-1.8)
P 		538.7
(-2.8)
P 		517.3
(-3)
P 		870.5
(0.6)
P 		1104.9
(3)
P 		847
(0.4)
P 		588
B 597.2
(-2.9)
P 		594.2
(-3)
P 		623.8
(-2.5)
P 		910.9
(2.4)
P 		945.2
(3)
P 		754.9
(-0.3)
P 		351
C 476.4
(-3)
P 		610.5
(-1.5)
P 		717.9
(-0.2)
P 		997.1
(3)
P 		923.7
(2.2)
P 		769.8
(0.4)
P 		521
avr 568.6
(-3) 		581.1
(-2.8) 		619.7
(-2.3) 		926.2
(2.1) 		991.3
(3) 		790.6
(0.2) 		423
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 191
1 2 3 4 range
A 313
(3)
P 		265.3
(1.2)
P 		207.5
(-1.1)
P 		157.4
(-3)
P 		156
B 309.1
(3)
P 		274.3
(1.2)
P 		191.4
(-3)
P 		275.6
(1.3)
P 		118
C 314
(1.9)
P 		348
(3)
P 		293.7
(1.3)
P 		156.8
(-3)
P 		191
avr 312
(3) 		295.9
(2.2) 		230.9
(-1.2) 		196.6
(-3) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 271
1 2 3 4 5 6 range
A 116.6
(-2.9)
P 		191.6
(-0.9)
P 		112.7
(-3)
P 		220.8
(-0.1)
P 		333.4
(3)
P 		206.2
(-0.5)
P 		221
B 207.8
(-1.5)
P 		197.8
(-1.8)
P 		151.5
(-3)
P 		241.3
(-0.7)
P 		384
(3)
P 		281.5
(0.4)
P 		233
C 176.6
(-3)
P 		186.3
(-2.5)
P 		182.7
(-2.7)
P 		200.5
(-1.8)
P 		298.9
(3)
P 		257.7
(1)
P 		122
avr 167
(-2.4) 		191.9
(-1.6) 		149
(-3) 		220.9
(-0.7) 		338.8
(3) 		248.5
(0.1) 		190
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187973_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11752
Sequence Type Exemplar sequence
Representative Public ID F22E12.4 NCBI
Target Description F22E12.4 /REP_DB=WormBase Gene ID /WP=CE27755 /GEN=egl-9 /TR=O45918 /GB=CAA16314.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028661(11), NM_171533(11), NM_001028662(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028661 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-9) (egl-9) mRNA, complete cds. 11/11 None
NM_171533 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-9) (egl-9) mRNA, complete cds. 11/11 None
NM_001028662 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-9) (egl-9) mRNA, complete cds. 11/11 None
SNAP00000025292 ENSEMBL cdna:SNAP chromosome:CEL140:V:10475919:10479045:1 11/11 None
GENEFINDER00000025297 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10475919:10479045:1 11/11 None
F22E12.4a ENSEMBL cdna:known chromosome:CEL140:V:10471097:10479534:1 gene:F22E12.4 11/11 None
F22E12.4b.1 ENSEMBL cdna:known chromosome:CEL140:V:10471099:10479523:1 gene:F22E12.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001028663 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179461 4 3 NM_001028661 187973_s_at (11)
NM_001028662 187973_s_at (11)
NM_171533 187973_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10471106-10479045(+) 100.0 100.0
Public Domain and Genome References
Ensembl F22E12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94893 Hypothetical protein F22E12.4a [Caenorhabditis elegans] emb|CAA16314.2| Hypothetical protein F22E12.4a [Caenorhabditis elegans] ref|NP_741621.1| EGg Laying defective family member (egl-9) [Caenorhabditis elegans] gb|AAD56365.1| EGL-9 [Caenorhabditis elegans] 0.0
blast CAI79160 Hypothetical protein F22E12.4d [Caenorhabditis elegans] emb|CAI79248.1| Hypothetical protein F22E12.4d [Caenorhabditis elegans] ref|NP_001023834.1| EGg Laying defective family member (egl-9) [Caenorhabditis elegans] 0.0
blast CAE64809 Hypothetical protein CBG09602 [Caenorhabditis briggsae] 0.0
blast CAI79247 Hypothetical protein F22E12.4c [Caenorhabditis elegans] ref|NP_001023833.1| EGg Laying defective family member (egl-9) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002893 EMBL-EBI Zn-finger, MYND type 3.4E-23
Pfam IPR005123 EMBL-EBI 2OG-Fe(II) oxygenase superfamily 1.9E-13
Pfam IPR005123 EMBL-EBI 2OG-Fe(II) oxygenase superfamily 1.9E-13
Pfam IPR005123 EMBL-EBI 2OG-Fe(II) oxygenase superfamily 1.9E-13
Pfam IPR005123 EMBL-EBI 2OG-Fe(II) oxygenase superfamily 1.9E-13
Sequence
Target Sequence 
>C. ELEGANS:187973_S_AT
tgatcgtctggtattcttctggtccgatcgtcgcaatcctcatgaagtcatgccagtctt
ccgtcatcgtttcgcaattactatttggtatatggataaatccgaaagagataaggcttt
ggcaaaaggaaaagagtcagatgcggcatgtgcttcaaagaaagagaatgatccaacaag
ctcttcactaaattcccttattggatcacttttgagaccacggaaaaatccaagtactca
cgatttatcaaaacttgaccttcgactcttcccgtccacatcatccgatccagctctggt
atctgcagcagatgaagatagagttgacatctctgccgactttcaatccacttcaagtct
ggctcatccggaatctactgactcgggagtatctctctccaccttcaatgtcgctcataa
tcacatggaacgtactaccagtctccagtcgatctccgatcatttccgttccgaaagatc
acacgaacgtcgcagctcaacaagcagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATCGTCTGGTATTCTTCTGGTCC 55 567 1821 Antisense
ATCGTCGCAATCCTCATGAAGTCAT 533 35 1847 Antisense
CATGAAGTCATGCCAGTCTTCCGTC 24 211 1861 Antisense
CAAGCTCTTCACTAAATTCCCTTAT 85 187 1997 Antisense
CATCCGATCCAGCTCTGGTATCTGC 304 211 2102 Antisense
TAGAGTTGACATCTCTGCCGACTTT 424 653 2139 Antisense
TCAAGTCTGGCTCATCCGGAATCTA 358 631 2173 Antisense
CTGACTCGGGAGTATCTCTCTCCAC 254 239 2198 Antisense
CCACCTTCAATGTCGCTCATAATCA 657 273 2219 Antisense
TGGAACGTACTACCAGTCTCCAGTC 68 545 2246 Antisense
CGAACGTCGCAGCTCAACAAGCAGC 422 253 2304 Antisense
Sequence Source Affymetrix Proprietary Database