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[187971_at]

Description C09G4.5 /REP_DB=WormBase Gene ID /WP=CE25773 /GEN=mes-6 /TR=Q9GYS1 /GB=AAF99868.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 76      4grp_highest_8_8 > 05      4grp_high_8_8 > 30      6grp_highest_nonorm > 93      6grp_total > 67      4grp_total > 25      6grp_highest_sub > Da      6grp_cgc > 48      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 248.9
(-3)
P 		362.1
(2.4)
P 		302.5
(-0.4)
P 		373.8
(3)
P 		125
B 318.5
(-1.9)
P 		334.2
(0.3)
P 		353.5
(3)
P 		310.6
(-3)
P 		43
C 290.3
(-3)
P 		297.6
(-2.7)
P 		332.6
(-1.1)
P 		425.5
(3)
P 		135
avr 285.9
(-3) 		331.3
(0.2) 		329.5
(0.1) 		370
(3) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 577
1 2 3 4 5 6 range
A 348.5
(-2.9)
P 		343.1
(-3)
P 		382.4
(-2.1)
P 		440.2
(-0.9)
P 		385.3
(-2.1)
P 		614.8
(3)
P 		272
B 350.3
(-2.4)
P 		312
(-2.8)
P 		291.7
(-3)
P 		367.8
(-2.2)
P 		395.3
(-1.9)
P 		843.3
(3)
P 		552
C 266.8
(-3)
P 		395.1
(-0.7)
P 		356.8
(-1.4)
P 		387
(-0.9)
P 		307
(-2.3)
P 		602.5
(3)
P 		336
avr 321.9
(-3) 		350.1
(-2.5) 		343.6
(-2.6) 		398.3
(-1.7) 		362.5
(-2.3) 		686.9
(3) 		365
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 90.6
(3)
P 		78.6
(1)
P 		79.1
(1.1)
P 		54.7
(-3)
P 		36
B 101.6
(3)
P 		82.5
(-0.2)
P 		81.9
(-0.3)
P 		66.2
(-3)
P 		35
C 96.1
(0.3)
P 		85.5
(-1.1)
P 		115.9
(3)
P 		71.7
(-3)
P 		44
avr 96.1
(3) 		82.2
(0.4) 		92.3
(2.3) 		64.2
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 250
1 2 3 4 5 6 range
A 64.2
(-3)
P 		122
(1.1)
P 		83.3
(-1.7)
P 		111.6
(0.3)
P 		116.3
(0.7)
P 		149.7
(3)
P 		86
B 121.9
(-1.7)
P 		103.9
(-2.2)
P 		70.8
(-3)
P 		97.4
(-2.3)
P 		160.6
(-0.8)
P 		314.5
(3)
P 		244
C 98.9
(-2)
P 		120.6
(-0.9)
P 		90.8
(-2.4)
P 		77.8
(-3)
P 		99.3
(-2)
P 		201.7
(3)
P 		124
avr 95
(-2.4) 		115.5
(-1.6) 		81.6
(-3) 		95.6
(-2.4) 		125.4
(-1.1) 		222
(3) 		140
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187971_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10200
Sequence Type Exemplar sequence
Representative Public ID C09G4.5 NCBI
Target Description C09G4.5 /REP_DB=WormBase Gene ID /WP=CE25773 /GEN=mes-6 /TR=Q9GYS1 /GB=AAF99868.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026149(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026149 NCBI Caenorhabditis elegans Maternal Effect Sterile family member (mes-6) (mes-6) mRNA, complete cds. 10/11 None
C09G4.5.1 ENSEMBL cdna:known chromosome:CEL140:IV:8510952:8513889:1 gene:C09G4.5 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001026148 3/11 Cross Hyb Matching Probes None
SNAP00000025640 7/11 Cross Hyb Matching Probes None
SNAP00000025642 3/11 Cross Hyb Matching Probes None
GENEFINDER00000025654 7/11 Cross Hyb Matching Probes None
GENEFINDER00000025656 3/11 Cross Hyb Matching Probes None
C09G4.5.2 7/11 Cross Hyb Matching Probes None
C09G4.4 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8511424-8513754(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mes-6
Ensembl C09G4.5
Entrez Gene 177657 Entrez gene
SwissProt Q9GYS1 EMBL-EBI
WormBase CE24796 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN84876 Maternal effect sterile protein 6 [Caenorhabditis elegans] ref|NP_001021320.1| Maternal Effect Sterile family member (mes-6) [Caenorhabditis elegans] sp|Q9GYS1|MES6_CAEEL Polycomb protein mes-6 (Maternal-effect sterile 6 protein) (ESC homolog) gb|AAC27121.1| WD-40 repeat protein [Caenorhabditis elegans] 0.0
blast T29827 hypothetical protein C09G4.5 - Caenorhabditis elegans 1.0E-158
blast CAE69384 Hypothetical protein CBG15499 [Caenorhabditis briggsae] 1.0E-95
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.022
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.4E-6
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.4
Sequence
Target Sequence 
>C. ELEGANS:187971_AT
aatgttctccgtccaaagtcgtgtacaacgaaatttcgtacaatgaatgttccgagtggt
tctgcatggtttatcaaattcgccgttgatccaagaagacgatggctggtgtgcggtgga
gctggtggttctgtgatgtttttcgatttgagaaataatgaagaaaccaatccgactcac
acttgttccgttggctcacgtacagttcggcaagcttctttctctacatgtggccgattc
ctagtactagtaactgacgaaggatttgtttgtcgatttgatcgtgtatctgcgtctgtc
gacgctaaggacctgttcctaatgtcatccccaacctcctctaaggattattcttcacct
aaacatagctttatgttgacagtcgacagcaacttacaaagtgacaaaaagaagacgttt
ttgaatgttcaatcatcttcctctgctgggtcaaccaagcactgtcaaattccaatgtca
aaggacgagtattttgaaacacgcgggaaactcgactctgttgatgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGTTCTCCGTCCAAAGTCGTGTA 414 163 1066 Antisense
GTTCCGAGTGGTTCTGCATGGTTTA 99 441 1114 Antisense
GAAGACGATGGCTGGTGTGCGGTGG 236 339 1160 Antisense
GGAGCTGGTGGTTCTGTGATGTTTT 551 521 1183 Antisense
GGCTCACGTACAGTTCGGCAAGCTT 65 537 1258 Antisense
TTTCTCTACATGTGGCCGATTCCTA 342 671 1284 Antisense
TTGATCGTGTATCTGCGTCTGTCGA 209 707 1343 Antisense
GTCTGTCGACGCTAAGGACCTGTTC 482 471 1359 Antisense
ATCCCCAACCTCCTCTAAGGATTAT 101 37 1392 Antisense
GGTCAACCAAGCACTGTCAAATTCC 285 503 1514 Antisense
GCGGGAAACTCGACTCTGTTGATGT 241 293 1568 Antisense
Sequence Source Affymetrix Proprietary Database