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[187965_s_at]

Description F12F3.3 /REP_DB=WormBase Gene ID /WP=CE27954 /TR=P91257 /GB=AAC25885.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=titin
Cluster 6grp_total > 02      4grp_total > 37      6grp_cgc > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 171
1 2 3 4 range
A 226.6
(3)
A 		157.2
(-0.3)
A 		100.9
(-3)
A 		206.4
(2)
A 		126
B 244
(3)
A 		170.6
(0.4)
A 		73.5
(-3)
A 		75.4
(-2.9)
A 		171
C 210.1
(3)
A 		189.1
(1.8)
A 		105.4
(-3)
A 		139.9
(-1)
A 		105
avr 226.9
(3) 		172.3
(0.5) 		93.3
(-3) 		140.6
(-0.9) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 104.7
(1.2)
A 		141.8
(3)
A 		18.9
(-3)
A 		64.9
(-0.8)
A 		71.5
(-0.4)
A 		44.1
(-1.8)
A 		123
B 75.4
(1.9)
A 		58.8
(0.4)
A 		19.2
(-3)
A 		88.6
(3)
A 		71.2
(1.5)
A 		43.6
(-0.9)
A 		69
C 79.3
(3)
A 		71.3
(2.1)
A 		75.9
(2.6)
A 		23.1
(-3)
A 		44.5
(-0.7)
A 		60.2
(1)
A 		56
avr 86.5
(2.5) 		90.6
(3) 		38
(-3) 		58.9
(-0.6) 		62.4
(-0.2) 		49.3
(-1.7) 		53
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 82.5
(3)
A 		34.1
(-2.2)
A 		26.4
(-3)
A 		30.2
(-2.6)
A 		56
B 77.8
(3)
A 		42.1
(-0.5)
A 		17
(-2.9)
A 		16.1
(-3)
A 		62
C 69.6
(3)
A 		54.4
(1)
A 		36.7
(-1.3)
A 		23.6
(-3)
A 		46
avr 76.6
(3) 		43.5
(-0.7) 		26.7
(-2.6) 		23.3
(-3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 19.3
(-1)
A 		50.4
(3)
A 		4.1
(-3)
A 		16.5
(-1.4)
A 		21.6
(-0.7)
A 		10.7
(-2.1)
A 		46
B 26.2
(2.3)
A 		19.6
(0.7)
A 		4.7
(-3)
A 		23.5
(1.7)
A 		28.9
(3)
A 		16.3
(-0.1)
A 		24
C 29.4
(3)
A 		21.8
(1.2)
A 		19.3
(0.6)
A 		4.6
(-3)
A 		14.4
(-0.6)
A 		20.2
(0.8)
A 		25
avr 25
(1.4) 		30.6
(3) 		9.4
(-3) 		14.9
(-1.4) 		21.6
(0.5) 		15.7
(-1.2) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187965_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12385
Sequence Type Exemplar sequence
Representative Public ID F12F3.3 NCBI
Target Description F12F3.3 /REP_DB=WormBase Gene ID /WP=CE27954 /TR=P91257 /GB=AAC25885.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=titin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029031(11), NM_001029033(11), NM_001029032(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029031 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-58) (tag-58) mRNA, complete cds. 11/11 None
NM_001029033 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-58) (tag-58) mRNA, complete cds. 11/11 None
NM_001029032 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-58) (tag-58) mRNA, complete cds. 11/11 None
W06H8.8e ENSEMBL cdna:known chromosome:CEL140:V:6123860:6205127:-1 gene:W06H8.8 11/11 A 
W06H8.8g ENSEMBL cdna:known chromosome:CEL140:V:6123881:6205127:-1 gene:W06H8.8 11/11 A 
W06H8.8f ENSEMBL cdna:known chromosome:CEL140:V:6124187:6205127:-1 gene:W06H8.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
266969 7 3 NM_001029029 183162_at (11)
NM_001029030 176443_s_at (10), 183162_at (11), 185778_at (11)
NM_001029031 174345_at (11), 176443_s_at (10), 183162_at (11), 185778_at (11), 187965_s_at (11), 187994_at (10)
NM_001029032 176443_s_at (10), 183162_at (11), 185778_at (11), 187965_s_at (11), 187994_at (10)
NM_001029033 174345_at (11), 176443_s_at (10), 183162_at (11), 185778_at (11), 187965_s_at (11), 187994_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6146879-6158581(-) 100.0 100.0
Public Domain and Genome References
Ensembl W06H8.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001024202 Temporarily Assigned Gene name family member (tag-58) [Caenorhabditis elegans] gb|AAN61517.1| 2MDa_1 protein [Caenorhabditis elegans] 0.0
blast NP_001024204 Temporarily Assigned Gene name family member (tag-58) [Caenorhabditis elegans] 0.0
blast NP_001024203 Temporarily Assigned Gene name family member (tag-58) [Caenorhabditis elegans] gb|AAN61518.1| 2MDa_2 protein [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-52
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-53
hanks 2.1.17 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; TWITCH 3.0E-85
Protein Domains
Database ID Description E-Value
scop a.2.7.Phenylalanyl-tRNA synthetase (PheRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Phenylalanyl-tRNA synthetase (PheRS) 4.30000019073486
scop a.2.7.Phenylalanyl-tRNA synthetase (PheRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Phenylalanyl-tRNA synthetase (PheRS) 4.19999980926514
Pfam IPR003961 EMBL-EBI Fibronectin, type III 9.3E-14
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.4E-17
Pfam IPR003961 EMBL-EBI Fibronectin, type III 4.3E-16
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.7E-11
Pfam IPR003961 EMBL-EBI Fibronectin, type III 7.4E-10
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.0E-14
Pfam IPR003961 EMBL-EBI Fibronectin, type III 7.9E-12
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.3E-6
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.4E-17
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.4E-12
Pfam IPR000719 EMBL-EBI Protein kinase 2.2E-83
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0015
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.017
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.5E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.093
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0041
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.7E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.077
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 5.3E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0026
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.2
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.89
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.48
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.15
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.1
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 9.2E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0025
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.7E-8
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.3E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 8.3E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 5.2E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0022
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.15
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.022
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.4E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.6E-8
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0017
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 8.4E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.1E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.71
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.8E-8
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.4E-10
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0037
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.4E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.0E-7
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.012
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0084
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-5
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.4E-12
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.022
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.4E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.6E-8
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0017
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 8.4E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.1E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.71
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.8E-8
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.4E-10
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0037
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.4E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 4.0E-7
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.012
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0084
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-5
Sequence
Target Sequence 
>C. ELEGANS:187965_S_AT
agccagaatatgtatcttccaccgcgacttcaattgcattgaaatggacatctgataacg
atgaagtcacatacaccgttcaaatgaaagaagccaattcaaagcgtccgtgggctgtag
tagccaaggaaatttcagaatgttctgctaccattgctcagctgaaggaaggaaccagct
acctgttccgagttatcgctcagaataaaactggtcaaactgtcacttccgagcaaagtg
aatcaattgaatgcaaagacactacagaatctaaaaagcctgcattcacaaatgctccaa
ccgatttgacagcagttaaaaacggaaagactaaaatcacagcagagttcaccggacatc
ctgcaccagaaattcattggtttaagaacaaaaaagaaattttctctggaaaacggcaat
ggattgaaaacatcgccggtgcaacatctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCCAGAATATGTATCTTCCACCGC 221 83 9923 Antisense
TTCCACCGCGACTTCAATTGCATTG 517 699 9939 Antisense
GAAGTCACATACACCGTTCAAATGA 2 337 9985 Antisense
AAAGCGTCCGTGGGCTGTAGTAGCC 435 119 10023 Antisense
TTCTGCTACCATTGCTCAGCTGAAG 187 693 10065 Antisense
CAGCTACCTGTTCCGAGTTATCGCT 393 203 10098 Antisense
AAACTGGTCAAACTGTCACTTCCGA 709 125 10130 Antisense
CATTCACAAATGCTCCAACCGATTT 38 217 10205 Antisense
AATCACAGCAGAGTTCACCGGACAT 134 171 10257 Antisense
ACCGGACATCCTGCACCAGAAATTC 264 89 10273 Antisense
GAAAACATCGCCGGTGCAACATCTT 462 355 10348 Antisense
Sequence Source Affymetrix Proprietary Database