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[187962_at]

Description K02E2.4 /REP_DB=WormBase Gene ID /WP=CE18839 /GEN=ins-35 /TR=Q9XUV3 /GB=CAB04546.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 27      6grp_high1_10_10 > 62      4grp_highest_8_8 > 20      4grp_high_8_8 > 31      6grp_highest_nonorm > 34      6grp_total > 62      4grp_total > 32      6grp_highest_sub > 03_04      6grp_cgc > 93      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1550
1 2 3 4 range
A 1031.6
(-3)
P 		1677.5
(-0.5)
P 		2466.1
(2.6)
P 		2581.6
(3)
P 		1550
B 1180.5
(-3)
P 		1759.1
(-0.5)
P 		2567.6
(3)
P 		2291.5
(1.8)
P 		1387
C 1182.9
(-3)
P 		1578.2
(-1)
P 		2162.4
(1.9)
P 		2372
(3)
P 		1189
avr 1131.7
(-3) 		1671.6
(-0.5) 		2398.7
(2.9) 		2415
(3) 		1283

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4728
1 2 3 4 5 6 range
A 792.5
(-3)
P 		1462.9
(-2.1)
P 		5431.3
(3)
P 		3736.5
(0.8)
P 		1451.9
(-2.1)
P 		959.8
(-2.8)
P 		4639
B 750
(-3)
P 		1692.2
(-1.4)
P 		4345.2
(3)
P 		3659.1
(1.9)
P 		1520
(-1.7)
P 		985.5
(-2.6)
P 		3595
C 703.7
(-3)
P 		1546.4
(-1.6)
P 		3978.2
(2.6)
P 		4203.3
(3)
P 		1348.3
(-1.9)
P 		1076.4
(-2.4)
P 		3500
avr 748.7
(-3) 		1567.2
(-1.7) 		4584.9
(3) 		3866.3
(1.9) 		1440.1
(-1.9) 		1007.2
(-2.6) 		3836
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 390
1 2 3 4 range
A 375.6
(-2.8)
P 		364.1
(-3)
P 		644.7
(3)
P 		377.9
(-2.7)
P 		281
B 376.4
(-3)
P 		434.5
(-1.4)
P 		595
(3)
P 		488.3
(0.1)
P 		219
C 391.6
(-3)
P 		453.8
(-2)
P 		753.7
(3)
P 		400
(-2.9)
P 		362
avr 381.2
(-3) 		417.5
(-2.2) 		664.5
(3) 		422.1
(-2.1) 		283

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1037
1 2 3 4 5 6 range
A 146.1
(-3)
P 		520.3
(-0.8)
P 		1182.8
(3)
P 		947.7
(1.6)
P 		438.1
(-1.3)
P 		233.7
(-2.5)
P 		1037
B 261
(-3)
P 		563.3
(-0.7)
P 		1055.1
(3)
P 		969.2
(2.4)
P 		617.6
(-0.3)
P 		367.5
(-2.2)
P 		794
C 260.8
(-3)
P 		471.9
(-1.3)
P 		1012.5
(3)
P 		845.3
(1.7)
P 		436.3
(-1.6)
P 		360.3
(-2.2)
P 		752
avr 222.6
(-3) 		518.5
(-0.9) 		1083.5
(3) 		920.7
(1.9) 		497.3
(-1.1) 		320.5
(-2.3) 		861
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187962_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14257
Sequence Type Exemplar sequence
Representative Public ID K02E2.4 NCBI
Target Description K02E2.4 /REP_DB=WormBase Gene ID /WP=CE18839 /GEN=ins-35 /TR=Q9XUV3 /GB=CAB04546.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075525(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075525 NCBI Caenorhabditis elegans INSulin related family member (ins-35) (ins-35) mRNA, complete cds. 11/11 None
SNAP00000027376 ENSEMBL cdna:SNAP chromosome:CEL140:V:20373591:20374260:1 11/11 None
GENEFINDER00000027387 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20373591:20374260:1 11/11 None
K02E2.4 ENSEMBL cdna:known chromosome:CEL140:V:20373589:20374352:1 gene:K02E2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20373590-20374260(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ins-35
Ensembl K02E2.4
Entrez Gene 180334 Entrez gene
SwissProt Q9XUV3 EMBL-EBI
WormBase CE18839 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04546 Hypothetical protein K02E2.4 [Caenorhabditis elegans] ref|NP_507926.1| INSulin related family member (ins-35) [Caenorhabditis elegans] 6.0E-40
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507926.1 1 4-23
K02E2.4 1 4-23
SNAP00000027376 1 4-23
GENEFINDER00000027387 1 4-23
Sequence
Target Sequence 
>C. ELEGANS:187962_AT
aatattcttggtaatccttgctgcctgcctcctcgcaataatcttggcgtcgccaactgg
aaaacaccacaaaatggatgagaacgcgtttggaatcaataatcgtcactgccaacgcgc
actaaaggtctattcatttgccatctgtggagcaatatgtcagaactatgaaaagattct
catggaaggatgtggctcaacggtgatgctcaccatgcaacgcacaaaacttatctgctg
cccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATATTCTTGGTAATCCTTGCTGCC 574 173 21 Antisense
GCCTCCTCGCAATAATCTTGGCGTC 386 283 47 Antisense
GGCGTCGCCAACTGGAAAACACCAC 527 513 66 Antisense
ATGGATGAGAACGCGTTTGGAATCA 436 51 94 Antisense
TCAATAATCGTCACTGCCAACGCGC 570 633 116 Antisense
GCCAACGCGCACTAAAGGTCTATTC 8 279 131 Antisense
GGTCTATTCATTTGCCATCTGTGGA 27 503 147 Antisense
GATTCTCATGGAAGGATGTGGCTCA 37 433 195 Antisense
ATGTGGCTCAACGGTGATGCTCACC 82 51 210 Antisense
GATGCTCACCATGCAACGCACAAAA 604 413 225 Antisense
CGCACAAAACTTATCTGCTGCCCAG 139 255 241 Antisense
Sequence Source Affymetrix Proprietary Database