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[187955_at]

Description C13G3.2 /REP_DB=WormBase Gene ID /WP=CE05278 /GEN=hot-6 /TR=Q17946 /GB=CAA98420.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 53      4grp_highest_8_8 > 14      4grp_high_8_8 > 14      6grp_highest_nonorm > 71      6grp_total > 20      4grp_total > 09      6grp_cgc > 55      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 361
1 2 3 4 range
A 186.4
(-3)
P 		313.7
(-0.1)
P 		276.3
(-0.9)
P 		445.9
(3)
P 		260
B 225.2
(-2.9)
P 		218.6
(-3)
P 		242.9
(-2.6)
P 		547.3
(3)
P 		329
C 212.8
(-3)
P 		221.4
(-2.8)
P 		281.5
(-1.7)
P 		525.5
(3)
P 		313
avr 208.1
(-3) 		251.2
(-2.1) 		266.9
(-1.8) 		506.2
(3) 		298

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 200
1 2 3 4 5 6 range
A 329.9
(-2.3)
P 		306.9
(-3)
P 		497.1
(3)
P 		320.4
(-2.6)
P 		354
(-1.5)
P 		317.2
(-2.7)
P 		190
B 347.3
(-2)
P 		404.8
(1.9)
P 		420.5
(3)
P 		365.6
(-0.7)
P 		340.2
(-2.5)
P 		332.5
(-3)
P 		88
C 296.9
(-3)
P 		465.2
(3)
P 		299.1
(-2.9)
P 		347.3
(-1.2)
P 		326.2
(-2)
P 		395.3
(0.5)
P 		168
avr 324.7
(-3) 		392.3
(2) 		405.6
(3) 		344.4
(-1.5) 		340.1
(-1.9) 		348.3
(-1.2) 		81
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 67.9
(-0.7)
P 		68.1
(-0.6)
P 		72.2
(3)
P 		65.3
(-3)
P 		7
B 71.8
(-1.3)
P 		54
(-3)
P 		56.3
(-2.8)
P 		116.6
(3)
P 		63
C 70.4
(-1.8)
P 		63.7
(-3)
P 		98.1
(3)
P 		88.6
(1.3)
P 		34
avr 70
(-1.3) 		61.9
(-3) 		75.5
(-0.1) 		90.2
(3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 60.8
(-3)
P 		109.1
(3)
P 		108.3
(2.9)
P 		81.3
(-0.5)
P 		106.8
(2.7)
P 		77.2
(-1)
P 		48
B 120.9
(0.5)
P 		134.8
(2.5)
P 		102.1
(-2.2)
P 		96.8
(-3)
P 		138.2
(3)
P 		124
(0.9)
P 		41
C 110
(0.3)
P 		142
(3)
P 		76.1
(-2.5)
P 		69.8
(-3)
P 		105.6
(-0)
P 		132.3
(2.2)
P 		72
avr 97.2
(-1.1) 		128.6
(3) 		95.5
(-1.3) 		82.6
(-3) 		116.9
(1.5) 		111.2
(0.7) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187955_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13835
Sequence Type Exemplar sequence
Representative Public ID C13G3.2 NCBI
Target Description C13G3.2 /REP_DB=WormBase Gene ID /WP=CE05278 /GEN=hot-6 /TR=Q17946 /GB=CAA98420.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073405(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073405 NCBI Caenorhabditis elegans Homolog of Odr-2(Two) family member (hot-6) (hot-6) mRNA, complete cds. 11/11 None
GENEFINDER00000001346 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11023706:11025461:-1 11/11 None
C13G3.2 ENSEMBL cdna:known chromosome:CEL140:V:11023706:11025461:-1 gene:C13G3.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000001342 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11023705-11025461(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hot-6
Ensembl C13G3.2
Entrez Gene 179525 Entrez gene
SwissProt Q17946 EMBL-EBI
WormBase CE05278 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA98420 Hypothetical protein C13G3.2 [Caenorhabditis elegans] ref|NP_505806.1| Homolog of Odr-2(Two) family member (hot-6) [Caenorhabditis elegans] gb|AAK01419.1| Ly-6-related protein HOT-6 [Caenorhabditis elegans] 1.0E-120
blast CAE75144 Hypothetical protein CBG23075 [Caenorhabditis briggsae] 2.0E-54
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505806.1 1 186-208
C13G3.2 1 186-208
GENEFINDER00000001346 1 186-208
Sequence
Target Sequence 
>C. ELEGANS:187955_AT
gctctacgtatttctgtttcattattttgcttgatatcaaatatatttgctaccagttgc
tttcattgtgtttcacaagacaaacagtacaatgcaacagttcgtcgacaacttgcaact
caaactgatattttcttttatccaattggtgtgaaatcacactattgcagtgaatccata
gatcctgaagatccacatatcattgaaggccaaatttgctcaaaatactcgatttgcgtt
acactgtttcccaatcttccagattctacatttgttgttcggggttgttttgagaacatt
cttcggcatagtcggagagctgaagaacaacttcaacaggatggatgttacttacttcga
tctcttccaatgtactccaacacagtttcaatggattacgtagtgtgtacgtgtaatggt
gactactgtaacacgatggaaatgcctgaagttgtcccaaaaccttattctttcggaaaa
tccaacattttaaaattgtcttacactaatgaaggcaatgcacttcaactttcctcgtct
tctttcagaatttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCTACGTATTTCTGTTTCATTAT 240 301 31 Antisense
ATTTGCTACCAGTTGCTTTCATTGT 514 17 75 Antisense
CAGTACAATGCAACAGTTCGTCGAC 161 195 115 Antisense
GTTCGTCGACAACTTGCAACTCAAA 662 441 130 Antisense
GATCCTGAAGATCCACATATCATTG 262 417 211 Antisense
GATTTGCGTTACACTGTTTCCCAAT 477 427 261 Antisense
GAGAACATTCTTCGGCATAGTCGGA 91 393 322 Antisense
GGATGTTACTTACTTCGATCTCTTC 544 513 373 Antisense
TCTCTTCCAATGTACTCCAACACAG 397 613 391 Antisense
ATGCCTGAAGTTGTCCCAAAACCTT 425 1 472 Antisense
TTTCCTCGTCTTCTTTCAGAATTTC 647 667 560 Antisense
Sequence Source Affymetrix Proprietary Database